2022 Fiscal Year Final Research Report
Identification of maturation-related pheromones in marine fish and the application of pheromone substances to aquaculture
| Project/Area Number |
20K21339
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
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| Research Institution | Nagasaki University |
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Principal Investigator |
Soyano Kiyoshi 長崎大学, 海洋未来イノベーション機構, 教授 (80260735)
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| Project Period (FY) |
2020-07-30 – 2023-03-31
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| Keywords | 成熟関連フェロモン / 成熟関連ホルモン / 成熟誘導 / 種苗生産技術 |
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| Outline of Final Research Achievements |
This study was conducted to identify and elucidate the role of maturation-related pheromones in marine fish.In this study, we detected proteins that appear during the maturation phase in the urine of male honeycomb grouper. As a result, it became clear that parvalbumin was present as a characteristic protein. The composition of free amino acids in urine was also analyzed, but no specific amino acids were found. Lipidomics analysis was performed to detect the most abundant lipid molecular species (LMS) in male urine. Among the LMS found in male urine, nine were classified as molecular species that were present at particularly high concentrations and were not detected in females. These were selected as pheromone candidates. Immunohistochemical examination of the response in the olfactory epithelium of females exposed to male urine revealed a positive response in crypt cells present in the olfactory epithelium of females. This indicates that females respond to odorants in male urine.
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| Free Research Field |
魚類生殖生理学
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| Academic Significance and Societal Importance of the Research Achievements |
魚類の成熟関連フェロモンは、いくつかの魚種で物質の同定が行われているに過ぎない。しかもその物質は構造的に多岐にわたる。本研究は魚類の繁殖において重要な役割を担うフェロモンの機能解明に向けた研究基盤構築としての意義が大きい。また、本研究は雄から放出される成熟関連フェロモンを「産卵を同調させる成熟誘導剤」として活用するというこれまでにない水産養殖技術の開発につながる画期的なアイデアを含んでいる。これが成功すればハタ類の種苗生産の作業効率の軽減と、より健全な種苗の生産に大いに貢献できる。本研究で得られ海産魚類のフェロモンに関する成果は、これらを実現する上で欠くことのできない知見である。
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