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2020 Fiscal Year Research-status Report

Development of the microscale stable isotope analytical technique of carbonate in fish hardparts for temperature reconstruction

Research Project

Project/Area Number 20K21343
Research InstitutionUniversity of Tsukuba

Principal Investigator

西田 梢  筑波大学, 生命環境系, 特任助教 (10708374)

Co-Investigator(Kenkyū-buntansha) 石村 豊穂  京都大学, 人間・環境学研究科, 准教授 (80422012)
白井 厚太朗  東京大学, 大気海洋研究所, 准教授 (70463908)
Project Period (FY) 2020-07-30 – 2022-03-31
Keywords安定同位体比 / 質量分析計 / 魚類 / 炭酸塩 / 温度復元 / 古環境復元 / 骨
Outline of Annual Research Achievements

脊椎動物の骨や歯の硬組織はリン酸塩を主成分とし、5-10%の炭酸基を含んでいる。この微量な炭酸塩の酸素安定同位体比は、変温動物であれば生息環境の温度や環境水を、恒温動物であれば体温や環境水を記録するので、その積極的な活用が可能になれば新たな生態情報の抽出が実現できる。しかしながら、魚類硬組織の多くは分析可能重量を満たせないために、これまでは同位体温度計の検証の術がなく、安定同位体比を活用したアプローチが実現できていなかった。本研究では、微量炭酸塩同位体比分析システム(世界で最も微量で同位体比分析が可能)の改良を行い、魚類硬組織(骨・歯など)から炭素・酸素同位体比情報を抽出し、環境・生態復元への貢献を目指す。

2020年度は、骨試料を活用してリン酸塩中炭酸塩の前処理方法や分析プロトコルの検証を行い、炭酸塩の安定同位体比を得る手法を確立することができた。また、骨試料の前処理による同位体比への影響評価を行い、有機物除去を目的とした骨試料への薬品処理では、薬品によっては骨中炭酸塩の同位体比に影響を与えることを明らかにした。骨試料を化学分析に用いる際には、薬品による有機物除去による分析値への影響を十分に検証する必要があることを示した。生物飼育実験試料として、温度飼育実験を実施した魚類個体や温度一定の条件下で飼育した海洋酸性化飼育実験の魚類個体を収集し、分析のための試料の剖出作業や飼育海水の同位体比分析を実施した。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

2020年度は、試料の前処理方法や分析プロトコルの検証を行い、炭酸塩の同位体比を得る手法を確立することができた。また温度情報が既知の魚類個体を収集し、分析のための試料の剖出作業や飼育海水の同位体比分析を実施した。研究は問題なく進展している。

Strategy for Future Research Activity

2021年度は、2020年度に確立した試料の前処理方法や同位体分析手法を基盤として、飼育温度がモニタリングされた2種の魚類飼育個体(温度飼育実験個体、温度一定の海洋酸性化飼育実験個体)を用いて、同位体温度計としての検証を行う。魚類の脊椎骨等の硬組織からデンタルドリルにより粉末試料の採取を行い、微量炭酸塩分析システムを用いて炭素・酸素安定同位体比分析を実施する。得られた分析データを基に、魚類硬組織中に含有する微量炭酸塩の温度依存性や同位体分別の種間差、代謝影響などについて議論を行う予定である。

Causes of Carryover

2020年度はCOVID-19の感染拡大に伴い、在宅勤務指示や緊急事態宣言による移動の制限等により、計画していたよりもフィールド調査や分析を目的とした出張、分析用消耗品の使用などが少なくなったため。次年度は、関係機関で協力しながら、早めの時期に分析作業を実施することでしっかりと分析・議論を行いたい。

  • Research Products

    (27 results)

All 2021 2020 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results,  Open Access: 6 results) Presentation (6 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Remarks (3 results)

  • [Journal Article] 耳石の元素・同位体比を自然指標として用いたまぐろ類の回遊生態研究2021

    • Author(s)
      天野洋典・横内一樹・石村豊穂・白井厚太朗
    • Journal Title

      水産海洋研究

      Volume: 85 Pages: 1~13

    • Peer Reviewed
  • [Journal Article] Identification of timing of scallop morphological deformity and mortality from shell oxygen isotope records2021

    • Author(s)
      Zhao Liqiang、Higuchi Tomihiko、Kanamori Makoto、Natsuike Masafumi、Misaka Naoyuki、Murakami-Sugihara Naoko、Tanaka Kentaro、Shirai Kotaro
    • Journal Title

      Marine Environmental Research

      Volume: 163 Pages: 105149~105149

    • DOI

      10.1016/j.marenvres.2020.105149

    • Peer Reviewed
  • [Journal Article] Divalent metal transporter-related protein restricts animals to marine habitats2021

    • Author(s)
      Sassa Mieko、Takagi Toshiyuki、Kinjo Azusa、Yoshioka Yuki、Zayasu Yuna、Shinzato Chuya、Kanda Shinji、Murakami-Sugihara Naoko、Shirai Kotaro、Inoue Koji
    • Journal Title

      Communications Biology

      Volume: 4 Pages: 1-9

    • DOI

      10.1038/s42003-021-01984-8

    • Peer Reviewed / Open Access
  • [Journal Article] Seasonal variation of total mercury transfer through a tropical mangrove food web, Setiu Wetlands2021

    • Author(s)
      Siau Yin Fui、Le Dung Quang、Suratman Suhaimi、Jaaman Saifullah Arifin、Tanaka Kentaro、Kotaro Shirai
    • Journal Title

      Marine Pollution Bulletin

      Volume: 162 Pages: 111878~111878

    • DOI

      10.1016/j.marpolbul.2020.111878

    • Peer Reviewed
  • [Journal Article] Reconstruction of temperature experienced by Pacific bluefin tuna Thunnus orientalis larvae using SIMS and microvolume CF-IRMS otolith oxygen isotope analyses2020

    • Author(s)
      Hane Y、Kimura S、Yokoyama Y、Miyairi Y、Ushikubo T、Ishimura T、Ogawa N、Aono T、Nishida K
    • Journal Title

      Marine Ecology Progress Series

      Volume: 649 Pages: 175~188

    • DOI

      10.3354/meps13451

    • Peer Reviewed / Open Access
  • [Journal Article] Microscale stable carbon and oxygen isotope measurement of individual otoliths of larvae and juveniles of Japanese anchovy and sardine2020

    • Author(s)
      Nishida Kozue、Yasu Ayaka、Nanjo Nobuaki、Takahashi Motomitsu、Kitajima Satoshi、Ishimura Toyoho
    • Journal Title

      Estuarine, Coastal and Shelf Science

      Volume: 245 Pages: 106946~106946

    • DOI

      10.1016/j.ecss.2020.106946

    • Peer Reviewed
  • [Journal Article] Novel reverse radioisotope labelling experiment reveals carbon assimilation of marine calcifiers under ocean acidification conditions2020

    • Author(s)
      Nishida Kozue、Chew Yue Chin、Miyairi Yosuke、Hirabayashi Shoko、Suzuki Atsushi、Hayashi Masahiro、Yamamoto Yuzo、Sato Mizuho、Nojiri Yukihiro、Yokoyama Yusuke
    • Journal Title

      Methods in Ecology and Evolution

      Volume: 11 Pages: 739~750

    • DOI

      10.1111/2041-210X.13396

    • Peer Reviewed / Open Access
  • [Journal Article] Otolith δ18O and microstructure analyses provide further evidence of population structure in sardine Sardinops sagax around South Africa2020

    • Author(s)
      Sakamoto Tatsuya、van der Lingen Carl D、Shirai Kotaro、Ishimura Toyoho、Geja Yonela、Peterson James、Komatsu Kosei
    • Journal Title

      ICES Journal of Marine Science

      Volume: 77 Pages: 2669~2680

    • DOI

      10.1093/icesjms/fsaa130

    • Peer Reviewed / Open Access
  • [Journal Article] Groundwater Anomaly Related to CCS-CO2 Injection and the 2018 Hokkaido Eastern Iburi Earthquake in Japan2020

    • Author(s)
      Sano Yuji、Kagoshima Takanori、Takahata Naoto、Shirai Kotaro、Park Jin-Oh、Snyder Glen T.、Shibata Tomo、Yamamoto Junji、Nishio Yoshiro、Chen Ai-Ti、Xu Sheng、Zhao Dapeng、Pinti Daniele L.
    • Journal Title

      Frontiers in Earth Science

      Volume: 8 Pages: 611010~611010

    • DOI

      10.3389/feart.2020.611010

    • Peer Reviewed / Open Access
  • [Journal Article] Does trace fossil size correspond with burrower population density? An example from the modern counterpart of the trace fossil Bichordites2020

    • Author(s)
      Seike Koji、Shirai Kotaro、Kubota Kaoru、Ota Yuki、Sassa Shinji
    • Journal Title

      Palaeogeography, Palaeoclimatology, Palaeoecology

      Volume: 557 Pages: 109946~109946

    • DOI

      10.1016/j.palaeo.2020.109946

    • Peer Reviewed
  • [Journal Article] Fundamental questions and applications of sclerochronology: Community-defined research priorities2020

    • Author(s)
      Trofimova Tamara、Alexandroff S. J.、Mette M. J.、Tray E.、Butler P.G.、Campana S.E.、Harper E.M.、Johnson A. L.A.、Morrongiello J.R.、Peharda M.、Schone B. R.、Andersson C.、Andrus C. F. T.、Black B. A.、Burchell M.、Carroll M.L.、DeLong K.L.,,,,,,Shirai K.、Thebault J.、Trueman C.、de Winter N.
    • Journal Title

      Estuarine, Coastal and Shelf Science

      Volume: 245 Pages: 106977~106977

    • DOI

      10.1016/j.ecss.2020.106977

    • Peer Reviewed
  • [Journal Article] Diverse downstream migration patterns of the anadromous Japanese grenadier anchovy Coilia nasus in the Chikugo River estuary and Ariake Sea, Japan2020

    • Author(s)
      Itakura Hikaru、Yokouchi Kazuki、Kanazawa Takahiro、Matsumoto Masahiro、Matoba Tatsuhito、Wakiya Ryoshiro、Shirai Kotaro、Ishimatsu Atsushi
    • Journal Title

      Regional Studies in Marine Science

      Volume: 39 Pages: 101436~101436

    • DOI

      10.1016/j.rsma.2020.101436

    • Peer Reviewed
  • [Journal Article] Sea-entry conditions of juvenile chum salmon Oncorhynchus keta that improve post-sea-entry survival: a case study of the 2012 brood-year stock released from the Kushiro River, eastern Hokkaido, Japan2020

    • Author(s)
      Honda Kentaro、Shirai Kotaro、Komatsu Shinji、Saito Toshihiko
    • Journal Title

      Fisheries Science

      Volume: 86 Pages: 783~792

    • DOI

      10.1007/s12562-020-01442-0

    • Peer Reviewed
  • [Journal Article] A record of Tetraroge nigra (Tetrarogidae) from Iriomote Island, southern Japan, with notes on its ecological aspects2020

    • Author(s)
      Sato Mao、Kobayashi Hirozumi、Iida Midori、Shirai Kotaro、Sasaki Kunio
    • Journal Title

      Ichthyological Research

      Volume: 68 Pages: 207~213

    • DOI

      10.1007/s10228-020-00763-5

    • Peer Reviewed
  • [Journal Article] Feeding habitats of juvenile reef fishes in a tropical mangrove?seagrass continuum along a Malaysian shallow-water coastal lagoon2020

    • Author(s)
      Quang Le Dung、Yin Fui Siau、Tanaka Kentaro、Suratman Suhaimi、Sano Yuji、Shirai Kotaro
    • Journal Title

      Bulletin of Marine Science

      Volume: 96 Pages: 469~486

    • DOI

      10.5343/bms.2018.0093

    • Peer Reviewed / Open Access
  • [Journal Article] Structural-morphological and sedimentary features of forearc slope off Miyagi, NE Japan: implications for development of forearc basins and plumbing systems2020

    • Author(s)
      Chang Jih-Hsin、Park Jin-Oh、Chen Tzu-Ting、Yamaguchi Asuka、Tsuru Tetsuro、Sano Yuji、Hsu Ho-Han、Shirai Kotaro、Kagoshima Takanori、Tanaka Kentaro、Tamura Chiori
    • Journal Title

      Geo-Marine Letters

      Volume: 40 Pages: 309~324

    • DOI

      10.1007/s00367-020-00636-w

    • Peer Reviewed
  • [Journal Article] Tracking long‐distance migration of marine fishes using compound‐specific stable isotope analysis of amino acids2020

    • Author(s)
      Matsubayashi Jun、Osada Yutaka、Tadokoro Kazuaki、Abe Yoshiyuki、Yamaguchi Atsushi、Shirai Kotaro、Honda Kentaro、Yoshikawa Chisato、Ogawa Nanako O.、Ohkouchi Naohiko、Ishikawa Naoto F.、Nagata Toshi、Miyamoto Hiroomi、Nishino Shigeto、Tayasu Ichiro
    • Journal Title

      Ecology Letters

      Volume: 23 Pages: 881~890

    • DOI

      10.1111/ele.13496

    • Peer Reviewed
  • [Journal Article] A review of transgenerational effects of ocean acidification on marine bivalves and their implications for sclerochronology2020

    • Author(s)
      Zhao Liqiang、Shirai Kotaro、Tanaka Kentaro、Milano Stefania、Higuchi Tomihiko、Murakami-Sugihara Naoko、Walliser Eric O.、Yang Feng、Deng Yuewen、Schone Bernd R.
    • Journal Title

      Estuarine, Coastal and Shelf Science

      Volume: 235 Pages: 106620~106620

    • DOI

      10.1016/j.ecss.2020.106620

    • Peer Reviewed
  • [Presentation] 生物源炭酸塩の同位体地球科学・実験生物学の高度化による環境生態指標の評価研究2020

    • Author(s)
      西田 梢
    • Organizer
      日本地球化学会 受賞講演会
    • Invited
  • [Presentation] 生物源炭酸塩の安定同位体比分析技術の進展―微量化と絶対水温指標の登場2020

    • Author(s)
      西田 梢,石村 豊穂
    • Organizer
      第15回バイオミネラリゼーションワークショップ
    • Invited
  • [Presentation] Links between shell chemistry and microstructure-A case study using Arctica islandica2020

    • Author(s)
      Ellen Schnabel,Kotaro Shirai,Naoko Murakami-Sugihara,Klaus Peter Jochum,Nils Hoche,Kozue Nishida,Bernd R Schone
    • Organizer
      European Geosciences Union (EGU) General Assembly Conference 2020
    • Int'l Joint Research
  • [Presentation] Unraveling the Secrets Recorded in the Chemistry of Bivalve Shells2020

    • Author(s)
      Bernd R. Schone,Ellen Schnabel,Nils Hoche,Naoko Murakami-Sugihara,Klaus Peter Jochum,Kozue Nishida,Melita Peharda,Kresimir Markulin,Hana Unvanovic,Kotaro Shirai
    • Organizer
      Goldschmidt 2020
    • Int'l Joint Research
  • [Presentation] Measurement of stable carbon and oxygen isotope ratios of individual otoliths of early developmental stage Japanese anchovy and sardine with the MICAL3c microscale stable isotope analytical system2020

    • Author(s)
      Kozue Nishida,Ayaka Yasu,Nobuaki Nanjyo,Motomitsu Takahashi,Satoshi Kitajima,Toyoho Ishimura
    • Organizer
      Goldschmidt 2020
    • Int'l Joint Research
  • [Presentation] Groundwater anomaly related to the 2018 Hokkaido Eastern Iburi earthquake in Northern Japan2020

    • Author(s)
      Yuji Sano, Takanori Kagoshima, Naoto Takahata, Kotaro Shirai, Jin-Oh Park, Tomo Shibata, Junji Yamamoto, Yoshiro Nishio, Sheng Xu, Ai-Ti Chen, Pinti Daniele
    • Organizer
      European Geosciences Union (EGU) General Assembly Conference 2020
    • Int'l Joint Research
  • [Remarks] Researchmap

    • URL

      https://researchmap.jp/koz_n/

  • [Remarks] Google scholar

    • URL

      https://scholar.google.co.jp/citations?user=fYAFfFoAAAAJ&hl=ja&oi=ao

  • [Remarks] Researchgate

    • URL

      https://www.researchgate.net/profile/Kozue_Nishida

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Published: 2021-12-27  

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