Establishment of isolating methods for hair follicle stem cells derived from bovine: toward the implimentation of made-to-order hormone preparations

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K21371
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 42:Veterinary medical science, animal science, and related fields
• Research Institution: Tottori University
• Principal Investigator: HIGUCHI Masashi 鳥取大学, 農学部, 准教授 (70614791)
• Project Period (FY): 2020-07-30 – 2022-03-31
• Keywords: ウシ / 下垂体 / 毛包 / 組織幹細胞 / 細胞分離 / 分化

Outline of Final Research Achievements

We attempted to isolate tissue stem cells in the anterior lobe of the bovine pituitary gland and hair follicles using methods established in mice. As a result, we succeeded in isolating and culturing tissue stem cells from the anterior lobe of the bovine pituitary gland. However, it was found that they could not be induced to differentiate into gonadotroph under the same conditions as mice. In addition, the number of cells required for primary culture could not be obtained from beard hair follicles in bovine.

Free Research Field

生殖分泌学

Academic Significance and Societal Importance of the Research Achievements

本研究では、ウシ下垂体前葉に存在する組織幹細胞を分離することに初めて成功した。下垂体前葉組織幹細胞は下垂体前葉ホルモンを産生する5種類の細胞への分化機構を調べるために不可欠なツールであるが、これまでに齧歯類以外で組織幹細胞を分離する技術は存在していなかった。本研究の成果により、ウシ下垂体における細胞分化機構の解明が進むこと、そして、その先には下垂体前葉ホルモンを体外生産する技術を開発することが期待できる。