Establishment of a simple and rapid method for assessing amyloid polymorphism for pathophysiological diagnosis

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K21396
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
• Research Institution: Kobe University
• Principal Investigator: Chatani Eri 神戸大学, 理学研究科, 准教授 (00432493)
• Project Period (FY): 2020-07-30 – 2023-03-31
• Keywords: 蛋白質 / ミスフォールディング / アミロイド / 多形

Outline of Final Research Achievements

This study has been conducted aiming to construct a simple and rapid method for identifying amyloid polymorphism. Through the analysis of insulin amyloid fibrils, we have demonstrated that iodine staining is useful for distinguishing amyloid polymorphs. Furthermore, as an application example of this method, we tracked the preservation and changes of the structure of amyloid fibrils during seeding reactions. We also tried iodine staining of multiple types of amyloid fibrils and confirmed the applicability of iodine staining to a wide range of proteins.

Free Research Field

タンパク質科学、生物物理学

Academic Significance and Societal Importance of the Research Achievements

アミロイド多形は、異なる病態や感染性をもたらす構造的な要因であることが指摘されているものの、迅速かつ鋭敏に識別する方法は見出されていなかった。ヨウ素染色はアミロイド線維の構造多形を幅広く識別することが示されたことから、現状を打開し、アミロイド研究での活用だけでなく新たな病態診断法への展開が期待される。