分泌性PLA2による新しいエクソソームバイオロジーの構築

2020 Fiscal Year Research-status Report

• Project/Area Number: 20K21550
• Research Institution: Tokai University
• Principal Investigator: 幸谷 愛 東海大学, 医学部, 教授 (00517477)
• Co-Investigator(Kenkyū-buntansha): 村上 誠 東京大学, 大学院医学系研究科(医学部), 教授 (60276607)
• Project Period (FY): 2020-07-30 – 2022-03-31
• Keywords: エクソソーム / sPLA2

Outline of Annual Research Achievements

COVID-19感染症の影響で、リピドミクス解析のみ進行したが、本年度は研究成果には結実しなかった。

Current Status of Research Progress

4: Progress in research has been delayed.

Reason

自粛期間中の研究の遅れ、更に飼育マウスの感染事故により、マウスのクリーニングが必要となったため、予定通りの研究の進捗が得られなかった。

Strategy for Future Research Activity

本年度の遅れを取り戻すために、人員を増やして、研究の推進に努め、エクソソームの全く新しい作用機序について分子生物学的に明らかにすると共に、その生物学的意義をマウスモデルを用いて、生体内で明らかにする。

Causes of Carryover

COVID-19パンデミック化によって本年度の研究計画はほぼ推進できなかったため、東海大学における本年度の使用予定研究費をそのまま、来年度に同じ用途で使用する予定である。

Research Products

• [Journal Article] Proteomic and phospholipidomic characterization of extracellular vesicles inducing tumor microenvironment in Epstein-Barr virus-associated lymphomas. (2021)
  • Author(s): Ito M, Kudo K, Higuchi H, Otsuka H, Tanaka M, Fukunishi N, Araki T, Takamatsu M, Ino Y, Kimura Y, Kotani A.
  • Journal Title: FASEB J. Volume: 35 Pages: e21505,
  • DOI: 10.1096/fj.202002730R.
  • Peer Reviewed
• [Journal Article] Expression of ERV3-1 in leukocytes of acute myelogenous leukemia patients. (2021)
  • Author(s): So Nakagawa, Masaharu Kawashima, Yuji Miyatake, Kai Kudo, Ryutaro Kotaki, Kiyoshi Ando, Ai Kotani.
  • Journal Title: Gene Volume: 773 Pages: 145363
  • DOI: 10.1016/j.gene.2020.145363.
  • Peer Reviewed
• [Journal Article] Extracellular vesicles secreted by HBV-infected cells modulate HBV persistence in hydrodynamic HBV transfection mouse model (2020)
  • Author(s): Masatoshi Kakizaki, Yuichiro Yamamoto, Motoyuki Otsuka, Kouichi Kitamura, Masatoshi Ito, Hideki Derek Kawai, Masamichi Muramatsu, Tatehiro Kagawa, Ai Kotani.
  • Journal Title: The Journal of biological chemistry Volume: 295(35) Pages: 12449 - 12460
  • DOI: 10.1074/jbc.RA120.014317.
  • Peer Reviewed
• [Journal Article] Overexpression of miR0669m inhibits erythroblast differentiation (2020)
  • Author(s): Ryutaro Kotaki, Masaharu Kawashima, Asuka Yamaguchi, Naoto Suzuki, Ryo Koyama-Nasu, Daisuke Ogiya, Kazuki Okuyama, Yuichiro Yamamoto, Masako Takamatsu, Natsumi Kurosaki, Kiyoshi Ando, Akihiko Murata, Masato Ohtsuka, So Nakagawa, Koko Katagiri, Ai Kotani.
  • Journal Title: Scientific reports Volume: 10(1) Pages: 13554
  • DOI: 10.1038/s41598-020-70442-y
  • Peer Reviewed
• [Journal Article] PD-L1/L2 protein levels rapidly increase on monocytes via trogocytosis from tumor cells in classical Hodgkin lymphoma. (2020)
  • Author(s): Masaharu Kawashima, Joaquim Carreras, Ai Kotani. et al.
  • Journal Title: Leukemia Volume: 34(9) Pages: 2405-2417
  • DOI: 10.1038/s41375-020-0737-9
  • Peer Reviewed
• [Journal Article] Interferon-γ induced PD-L1 expression and soluble PD-L1 production in gastric cancer. (2020)
  • Author(s): Yushi Imai, Tetsuhiro Chiba, Takayuki Kondo, Hiroaki Kanzaki, Kengo Kanayama, Junjie Ao, Ryuta Kojima, Yuko Kusakabe, Masato Nakamura, Tomoko Saito, Ryo Nakagawa, Eiichiro Suzuki, Shingo Nakamoto, Ryosuke Muroyama, Akinobu Tawada, Tomoaki Matsumura, Tomoo Nakagawa, Jun Kato, Ai Kotani, Hisahiro Matsubara, Naoya Kato.
  • Journal Title: Oncology letters Volume: 20(3) Pages: 2161-2168
  • DOI: 10.3892/ol.2020.11757
  • Peer Reviewed
• [Journal Article] Heat-Killed Fusobacterium nucleatum Triggers Varying Heme-Related Inflammatory and Stress Responses Depending on Primary Human Respiratory Epithelial Cell Type. (2020)
  • Author(s): Ryo Koike, Marni E Cueno, Keiko Nodomi, Muneaki Tamura, Noriaki Kamio, Hajime Tanaka, Ai Kotani, Kenichi Imai.
  • Journal Title: Molecules Volume: 25(17) Pages: 3839
  • DOI: 10.3390/molecules25173839
  • Peer Reviewed
• [Journal Article] PD-L1 is induced on hepatocyte surface via CKLF-like MARVEL transmembrane domain-containing protein 6 upregulation by the anti-HBV drug Entecavir. (2020)
  • Author(s): Yuichiro Yamamoto, Masatoshi Kakizaki, Ai Kotani. et al.
  • Journal Title: International immunology. Volume: 32(8) Pages: 519-531
  • DOI: 10.1093/intimm/dxaa018
  • Peer Reviewed
• [Journal Article] Dasatinib exacerbates splenomegaly of mice inoculated with Epstein-Barr virus-infected lymphoblastoid cell lines. (2020)
  • Author(s): Ryutaro Kotaki, Masaharu Kawashima, Yuichiro Yamamoto, Hiroshi Higuchi, Etsuko Nagashima, Natsumi Kurosaki, Masako Takamatsu, Yara Yukie Kikuti, Ken-Ichi Imadome, Naoya Nakamura, Ai Kotani.
  • Journal Title: Scientific reports Volume: 10(1) Pages: 4355
  • DOI: 10.1038/s41598-020-61300-y
  • Peer Reviewed