2022 Fiscal Year Final Research Report

Immune-pathological diagnostic artificial intelligence development research for pulmonary fibrosis using fibrotic foci-specific enhanced micro-CT

Research Project

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Project/Area Number	20K21599
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 53:Organ-based internal medicine and related fields
Research Institution	Fujita Health University (2022) Nagoya University (2020-2021)
Principal Investigator	Hashimoto Naozumi 藤田医科大学, 医学部, 教授 (30378020)
Co-Investigator(Kenkyū-buntansha)	若原恵子名古屋大学, 医学部附属病院, 講師 (00631433) 阪本考司名古屋大学, 医学部附属病院, 病院講師 (00635633) 中村彰太名古屋大学, 医学部附属病院, 講師 (20612849) 古川大記名古屋大学, 医学部附属病院, 特任助教 (30837654)
Project Period (FY)	2020-07-30 – 2023-03-31
Keywords	肺線維症 / 線維芽細胞 / 金ナノ粒子 / マイクロCT / 血管内皮細胞
Outline of Final Research Achievements	Although evaluation by conventional contrast-enhanced CT with X-ray contrast agents provides useful diagnostic information in clinical setting, the aim of this study is to identify lung disease-specific molecules using high-resolution and high-resolution radiographic images derived from contrast-enhanced micro-CT scans with gold nanoparticles as X-ray contrast agents. In this study, we were able to set micro-CT imaging to detect gold nanoparticle-bound lung-specific antibodies in the lung tissue by producing gold nanoparticle-bound lung-specific antibodies and intravenously administering them to living mice. In the future, administration of adjusted dose of gold nanoparticle-bound lung-specific antibody and adequate detection setting of gold nanoparticles bound to lung tissue by micro-CT might give micro-CT immunopathological diagnostic ability.
Free Research Field	呼吸器内科学
Academic Significance and Societal Importance of the Research Achievements	マイクロCTスキャンの臨床応用が期待される中、X線造影物質である金ナノ粒子標識抗原特異的抗体により組織内での標的抗原検出を可能にすることはマイクロCTスキャンに免疫病理学的診断能を付与することができ、侵襲的な検査を最小限にするという患者のアンメットニーズニーズの解消につながることが期待される。5~10年後の実臨床への応用を見据えた患者のunmetニーズを解消する挑戦的研究であり、本研究が得た研究基盤は、対象疾患に疾患特異的病理所見・疾患特異的抗原・その特異的抗体がある場合に応用でき、既存の学術の体系や方向を大きく変革・転換させる潜在性を有する。