2021 Fiscal Year Final Research Report

Retrospective quantitative analysis of juvenile stress using dentin matrix

Research Project

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Project/Area Number	20K21668
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 57:Oral science and related fields
Research Institution	Tohoku University
Principal Investigator	YAMADA SATORU 東北大学, 歯学研究科, 教授 (40359849)
Co-Investigator(Kenkyū-buntansha)	鈴木茂樹東北大学, 大学病院, 講師 (30549762) 齋藤正寛東北大学, 歯学研究科, 教授 (40215562) 根本英二東北大学, 歯学研究科, 准教授 (40292221) 齋藤幹東北大学, 大学病院, 講師 (40380852) 土屋志津広島大学, 医系科学研究科(歯), 助教 (60610053)
Project Period (FY)	2020-07-30 – 2022-03-31
Keywords	幼若期ストレス / 象牙質
Outline of Final Research Achievements	Mice were intravenously administrated corticosterone at early childhood or juvenile stage and the amount of corticosterone in mineralized hard tissue of their teeth was quantified by ELISA method. The results revealed that corticosterone was detected in mineralized hard tissue of the teeth of corticosterone-injected group but not PBS-injected control group. This result suggested that systemically administrated corticosterone could be deposited into hard tissue of teeth. Corticosterone level in circulation of the mice received early life stress was significantly higher than that of control mice (p=0.0019). Corticosterone level in hard tissue of the teeth of adult mice that have experienced early life stress was 0.5343 pg/mg and that of control adult mice was 0.3547 mg/kg (p = 0.2046). These results indicated that corticosterone induced by early life stress at early childhood stage possibly accumulated in hard tissue of the teeth and corticosterone level was retained during the lifespan.
Free Research Field	歯科保存学
Academic Significance and Societal Importance of the Research Achievements	本研究では、幼若期循環ストレスホルモン量が増加するマウスモデルにおいて、成体から回収される象牙質を分析し、幼若期循環ストレスホルモンが歯に沈着している可能性を示した。将来的には、さらに高度な微量検出法の使用により、歯に沈着しているストレスホルモンを測定することで、幼若期のストレス負荷や生育環境による心理的負荷を時系列に沿って遡及的かつ定量的に解析できる可能性がある。歯は咀嚼と審美性という機能を持つが、非侵襲的な幼若期全身循環状態の記録媒体としての新たな価値創出の端緒となる知見が得られた。