2022 Fiscal Year Final Research Report
Construction of the production system of novel useful materials by thermo-adaptive evolved E. coli
| Project/Area Number |
20K21868
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 64:Environmental conservation measure and related fields
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大井 俊彦 北海道大学, 工学研究院, 准教授 (40223713)
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| Project Period (FY) |
2020-07-30 – 2023-03-31
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| Keywords | バイオプラスチック / 大腸菌 / 高温 |
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| Outline of Final Research Achievements |
High-temperature-adapted evolutionary E. coli up to 47.9°C-adapted E. coli was made possible for bioplastic synthesis culture, and bioplastic (Polyhydroxybutylate: PHB) production at 43°C, the highest temperature in the world, was successfully achieved using 46°C- and 47.4°C-adapted E. coli. We have constructed a high-temperature-adapted evolutionary chaperonin GroEL/ES expression system, which is important for functional expression of PHB synthase, in JM109, a general-purpose bioplastic production host, and constructed a system capable of studying PHB production at 37-40°C. We identified a novel bacterium of the genus Ralstonia capable of PHB synthesis at 43°C and determined its entire Genomes were determined and bioplastic synthase groups phaC, A, and B were identified.
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| Free Research Field |
分子細胞進化学
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| Academic Significance and Societal Importance of the Research Achievements |
一般的な大腸菌の致死温度を超える世界最高温度43℃での大腸菌を用いたバイオプラスチック生産が可能となった。これにより、より硬度の高いバイオプラスチック生産への応用が可能となる。
高温でのバイオプラスチック生産に重要なプロテオスタシス系変異が存在することが示され、この遺伝子を発現させることで、汎用宿主大腸菌を用いた高温での有用物質生産系の構築の検討が可能となった。
新規バイオプラスチック生産菌を同定し、43℃以上の高温で機能可能なバイオプラスチック生産酵素遺伝子phaC,A,Bが同定された。今後、大腸菌を用いてより高温でバイオプラスチック生産が可能となる可能性が開かれた。
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