2022 Fiscal Year Final Research Report
Analysis of membrane protein function using extracellular vesicles and microhole devices
Project/Area Number |
20K21903
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 90:Biomedical engineering and related fields
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Research Institution | Kyushu Institute of Technology |
Principal Investigator |
YASUDA Takashi 九州工業大学, 大学院生命体工学研究科, 教授 (80270883)
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Project Period (FY) |
2020-07-30 – 2023-03-31
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Keywords | 細胞外ベシクル / 微小孔 / マイクロデバイス |
Outline of Final Research Achievements |
An array of microholes with a diameter of several μm was formed on a silicon nitride membrane with a thickness of approximately 1 μm. Human B lymphocyte cells were immobilized on the membrane. Sodium butyrate was applied to induce apoptosis of the cells, which permitted giant extracellular vesicles of more than 10 μm in diameter to be generated from the cells and protrude downward from the microholes. Next, two microhole array membranes were placed above and below each other with a gap of approximately 5 μm. Cells were immobilized on the top surface of the upper membrane, and giant vesicles were generated and sandwiched between the membranes. After the upper and lower membranes were separated, the vesicle membranes were transferred to the top surface of the lower membrane. In this way, we developed a technique to equip the microholes of a device with membrane proteins having normal structure and function while holding them on vesicle membranes.
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Free Research Field |
バイオマイクロデバイス
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Academic Significance and Societal Importance of the Research Achievements |
膜タンパク質は、細胞間情報伝達などで重要な役割を果たしていることから、有力な創薬ターゲットである。薬効と安全性が高い革新的な新薬を開発するには、膜タンパク質の機能をより詳細に解析する必要がある。しかしながら、構造が複雑で多様な膜タンパク質をその構造と機能を維持した状態でデバイス上に取得し、その機能を解析することは極めて困難であった。本研究の成果はこの課題を解決するものであり、創薬研究に新たな手法を提供し、新薬開発への大きな貢献を期待できる。
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