Trace detection in equilibrium system using ultrasound standing wave

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K22545
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0502:Inorganic/coordination chemistry, analytical chemistry, inorganic materials chemistry, energy-related chemistry, and related fields
• Research Institution: University of Tsukuba
• Principal Investigator: Akihisa Miyagawa 筑波大学, 数理物質系, 助教 (80881599)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: 微量計測 / 超音波 / マイクロ粒子 / DNA

Outline of Final Research Achievements

Novel trace detection schemes using ultrasound standing wave were proposed. In the in situ measurement of target on a particle surface, entrapment of single microparticle in the flow cell was achieved. In the detection scheme using hollow particles, the dynamic change in levitation coordinate of the particle, which allows us to detect the target with high sensitivity, was observed through the binding of the hollow microparticles and gold nanoparticles. In the detection scheme based on the bond breaking between the microparticle and glass plate, several thousand molecules of DNA molecule were detectable. Moreover, the semi-quantification of binding constant for the complex between the microparticle and glass plate was demonstrated.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

本研究では、分析化学の分野では定性的にしか利用されてこなかった超音波を定量的な微量計測法に応用している。これにより、音響場を利用している研究者や分析化学者に学術的な影響を与えることができる。また、数千分子の微量計測はPCR法のような増幅を用いずに行うことが可能である。計測はシンプルな装置によって構成されており、コンパクトな計測装置として構築可能である。したがって、医療などの現場でも簡単に活躍できる手法となると考えている。