2020 Fiscal Year Research-status Report
Control of Laminar flow in open space for subcellular operation
Project/Area Number |
20K22555
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Research Institution | Tokyo Metropolitan University |
Principal Investigator |
毛 思鋒 東京都立大学, 都市環境学部, 助教 (40885315)
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Project Period (FY) |
2020-09-11 – 2022-03-31
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Keywords | 5-nozzle chemical pen / Laminar flow / Comsol simulation / Cell research / Cell staining |
Outline of Annual Research Achievements |
The 5-nozzle chemical pen has been sucessfully fabricated using heat puller. On the microscope, we holded the chemical pen using an XYZ stage. The position of the device could be accurately contolled. A 3D simulation carried out using Comsol Multiphysics 5.3b indicated that the 5-nozzled could generate a narrow flow layer of the solution injected from the central nozzle. By change the central nozzle a little away from the geometry center, we could obtained extremly narrow flow layer with a width less than 10 um.We have examed the formation of the laminar flow using solution with fluorescein in the central nozzle. the results were consistant with those from the simulations.Preliminary test on cell have been carried out.
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Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
All research plan in the application proposal have finished or have preliminary test. The simulation results were satisfactory. By a little change on the position of the central nozzle, we obtained extremely narrow flow layer. The size of the central flow layer was much narrow than than in the original design. In the fluorescein experiments, we obtained stable laminar flow with the central flow layer in the center. The stability of the laminar was checked with the time. By adjusting the experiment parameters, the width of the central flow layer could be less than 10 um. Moreover, the preliminary test on single cell has been sucessful.
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Strategy for Future Research Activity |
Based on the current results, we will carry out partial cell staining using molecular probe. And, by loading Calcium probe in the cell, we will apply a drug stimuli to open the calcium ionchannel for calcium ion transport. Additionally, we will carry out cell cutting by using lysis buff as the central layer.
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Causes of Carryover |
We will carry out cell studies of this proposal. We need buy cells from company, culture cells. Those will need much cost. Additionally, we will buy cell staining probes, live/dead kit and so on. Those cell staining probes will need the money. We plan to buy new syringe pumps to meeting the requirements of the experiments, because now using pumps are not stable enough.
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Research Products
(1 results)