Control of Laminar flow in open space for subcellular operation

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K22555
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0502:Inorganic/coordination chemistry, analytical chemistry, inorganic materials chemistry, energy-related chemistry, and related fields
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: MAO SIFENG 東京都立大学, 都市環境科学研究科, 助教 (40885315)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: Microfluidics, / Microchemical Pen, / Laminar flow, / Single cell, / Lactate, / Subcellular

Outline of Final Research Achievements

Firstly, we developed a push-pull nozzle system for in situ single-cell stimulation and the real-time electrochemical monitoring of lactate
secreted from the cell. Glucose solution was continuously supplied to the cell and the lactate secreted from the cell was collected and transport to the aspiration channel for electrochemical detection. Rapid and sensitive detection of lactate changes from a single cell was achieved by amperometry. The results was pubilished on Analytical Chemistry.
Then, we developted a five-nozzle chemical pen to explore the communication between cells by precise manipulation of a part of the cell. By controlling the fluid velocity at each nozzle, a thin effective region was formed in the central region. The fluid in the effective region was stable and had high spatial resolutions enough to stimulate and operate a limited region of a single cell without affecting the other parts.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

We developed new technology for cell analysis. It will be a powerful tool for scientists to carry our single cell researches and cell molecular science.
And the achieved single cell analysis will benefit for disease diagnosis, especially for cancer diagnosis.