Characterization of novel microbial strains with high metabolic function of acetovanillone

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K22560
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0601:Agricultural chemistry and related fields
• Research Institution: Hirosaki University
• Principal Investigator: Higuchi Yudai 弘前大学, 農学生命科学部, 助教 (70880045)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: lignin / acetophenone / acetovanillone / RNA-Seq / efflux pomp

Outline of Final Research Achievements

Acetovanillone (AV) is a major aromatic monomer produced in oxidative lignin depolymerization, and acetophenones such as AV are known to exhibit antibacterial properties. In this study, we investigated the MHK4, which is a novel microbial strain with high metabolic function of AV. It was identified that AV was degraded by a novel enzymatic system in MHK4. In addition, RNA-Seq analysis revealed that genes, encoding the efflux pump, were highly induced during MHK4 growth with AV. This fact suggested that these efflux pump genes may contribute to high metabolic function of AV for MHK4.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

本研究により、バクテリアによる新規のアセトバニロン (AV) 変換酵素システムが明らかにされた。AVはリグニンの酸化分解により主要に生成するため、本研究で得られた成果はリグニンからの有用物質生産系の構築に貢献することが期待される。また本研究で見出されたEfllux pomp遺伝子はAVなどの芳香族化合物への耐性を高める因子として機能する可能性があるため、今後このEfllux pomp遺伝子の機能を明らかにすることで、微生物に芳香族化合物の耐性を付与するためのツールとして利用できる可能性がある。