2021 Fiscal Year Annual Research Report
Structural and functional studies of iron uptake ATP-binding cassette transporters (ABC transporters) in Gram-negative bacteria
| Project/Area Number |
20K22561
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| Research Institution | The University of Tokyo |
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Principal Investigator |
陸 鵬 東京大学, 大学院農学生命科学研究科(農学部), 助教 (20880339)
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| Project Period (FY) |
2020-09-11 – 2022-03-31
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| Keywords | VmFbpA / Membrane Protein / ABC transporter / Vibrio. species / Iron |
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| Outline of Annual Research Achievements |
Rosmarinic acid (RA) was identified as an iron uptake inhibitor of Vibrio bacteria. RA binds more tightly than Fe3+ to VmFbpA, the Vibrio ferric ion binding protein.RA competitively inhibits Fe3+ binding to VmFbpA, and thus inhibits Vibrio growth. Sodium citrate (SC) markedly enhances the RA’s effect to inhibit Vibrio growth. The RA/SC mixture effectively inhibits the growth of vibriosis causative pathogens but has little effect on E. coli. The crystal structures of VmFbpA complexed with RA and in the absence of RA were solved by X-Ray crystallography.
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