A novel approach to establish temperature sensitive mutants through reverse genetics and the functional analysis

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K22572
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0601:Agricultural chemistry and related fields
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Takumi TOMOI 基礎生物学研究所, 生命熱動態研究室, 研究員 (70880996)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: 温度生物学 / 植物生理学

Outline of Final Research Achievements

Different two species may show different physiological responses at the same temperature, when they have been adapted to different temperature environments. In this study, physiological responses of the model plant of Arabidopsis thaliana possessing genes derived from plants in different temperature environments are comparatively analyzed under different temperatures. Around 10 plants from different temperature environments were obtained, then ready for comprehensive gene sequencing, and now further research is underway. In addition, we have established a method that combines microscopic imaging and mathematical analysis to properly evaluate the response to different temperatures using plants such as Arabidopsis thaliana.

Free Research Field

植物生理学

Academic Significance and Societal Importance of the Research Achievements

生物は様々な環境で進化してきた。そのため、生物がもつ遺伝子は、それぞれの環境で効率よく働けるように最適化されてきたと考えられる。本研究では、異なる温度環境で生育する植物の相同遺伝子を入れ替えることで、温度に対する応答を変化させる手法の確立を目指す。そのための遺伝的資源を確保し、温度に対する応答を的確に評価する顕微鏡イメージングと数理解析の手法を確立した。