2021 Fiscal Year Final Research Report
Comprehensive analysis of genomic regions involved in gene expression regulation related to juvenile-adult phase transition
Project/Area Number |
20K22585
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund |
Review Section |
0602:Agricultural and environmental biology and related fields
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
Ogo Yuko 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, 主任研究員 (90572214)
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Project Period (FY) |
2020-09-11 – 2022-03-31
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Keywords | イネ / 出穂 |
Outline of Final Research Achievements |
For all crops, the timing of the juvenile-adult phase transition is important for yield and quality optimization. Rice, a model crop, is thought to enable a strict critical daylength response by integrating light and circadian clock signals. The purpose of this study is comprehensively analyzing the enhancer elements involved in gene expression control by light and circadian clock and clarify the mechanism of gene expression that controls juvenile-adult phase transition by combining a few NGS analyzes. ChIP-seq with phytochrome showed time-specific peaks upstream of some genes that are involved in light responses and circadian clock, although peaks of genes expressing in minor cells were not detected. In the future, more detailed analysis will be required, such as by single cell analysis.
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Free Research Field |
生物学
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Academic Significance and Societal Importance of the Research Achievements |
植物の相転換に関する分子機構は、イネやシロイヌナズナなどに詳しく解析されているものの、非コーディング領域に着目したものは少なく包括的な研究が求められていた。また、光と概日時計は代謝や細胞伸長など生命活動の根幹を担う多くの活動に作用するため、本研究のデータは相転換だけでなく様々な研究分野にイネのクロマチン状態に関する情報を提供できる。近年、エンハンサー領域を用いた育種が注目されており、予測した機能性エレメントにゲノム編集を用いて変異や欠失を起こすことにより、出穂や収量性に関わる育種リソースや作物そのものの開発に寄与できる。
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