Development of environment-friendly amine detection reagents that contribute to achieving SDGs and their application to peptide synthesis

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K22721
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0801:Pharmaceutical sciences and related fields
• Research Institution: Showa Pharmaceutical University
• Principal Investigator: Umeno Tomohiro 昭和薬科大学, 薬学部, 特任助教 (40879524)
• Project Period (FY): 2020-09-11 – 2023-03-31
• Keywords: アミン検出 / ペプチド / ドナー・アクセプター / 固相合成 / 酸塩基反応

Outline of Final Research Achievements

In this study, we developed a novel quantitative and non-destructive colorimetric amine detection method based on the acid-base reaction of the hydrochloride of electron donor-acceptor-type dyes with amines. In this method, a non-colored solution of HCl salt of dyes, whose pKBH+ value was carefully modified was deprotonated by amines on resin, and colored yellow. By measuring the absorption spectra of the colored solution, a good linear relationship (R2 = 0.998) with amine groups on the resin was confirmed.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

本研究により得られた成果は、近年注目されている中分子医薬の１つであるペプチドの効率的合成への貢献が期待される。また、従来法では検出できなかったアミノ酸の検出を定量的に行えるため、カップリング反応の試薬量の低減や時間の短縮、さらにアミン検出試薬の再利用など現代の科学研究に重要とされるSDGsに対応したペプチド合成を実現することが可能である。