2021 Fiscal Year Annual Research Report
Molecular dynamics of the fusion oncoprotein EML4-ALK in lung cancer as revealed by high-speed AFM
| Project/Area Number |
20K22837
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| Research Institution | Kanazawa University |
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Principal Investigator |
HAN XUJUN 金沢大学, ナノ生命科学研究所, 特任助教 (30872163)
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| Project Period (FY) |
2020-09-11 – 2022-03-31
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| Keywords | EML4-ALK / HS-AFM / IDR / monomer / dimer |
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| Outline of Annual Research Achievements |
As an intrinsically disordered region (IDR), the overall structure of EML4-ALK remains unclear. This study imaged the dynamic structures of variant EML4-ALK fusions using a high-speed atomic force microscope. Our data suggested that EML4-ALK displayed monomer, dimer, and trimer in solution conditions. Variant EML4-ALK displayed different monomeric profiles which guided structural differences in trimers, especially in the trimers of variant-3. Thus, this study might reveal a new mechanism of the specific chemoresistance based on the structural dynamics.
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