Comprehensive analysis of mucin expression and immune infiltrates in human cancer.

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K22961
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0905:Surgery of the organs maintaining homeostasis and related fields
• Research Institution: Jichi Medical University
• Principal Investigator: Shiba Satomi 自治医科大学, 医学部, 助教 (70721603)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: ムチン / 腫瘍浸潤リンパ球 / 乳癌 / 多重免疫染色 / 固形腫瘍 / 腫瘍関連マクロファージ

Outline of Final Research Achievements

Using image cytometric analysis technology by multiple immunostaining, we investigated the expression pattern of various Mucin and tumor-infiltrating immune cells in resected specimens of 25 breast cancers without diabetes and 25 breast cancers without diabetes matched by probability score. The complications of diabetes did not result in significant differences in the mode of expression of each Mutin, but increased the proportion of infiltrating M2 macrophages and decreased the proportion of CD8-positive T lymphocytes, resulting in changes in the immunological microenvironment. It was suggested that diabetes may affect the pathophysiology of breast cancer and the prognosis of patients.

Free Research Field

腫瘍外科学

Academic Significance and Societal Importance of the Research Achievements

乳癌は年々罹患数が増加しており、女性癌罹患率の1位を占めている。糖尿病は乳癌発症のリスク因子であるとともに乳癌の予後不良因子であることが報告されている。本研究結果は、糖尿病ががん微小環境を腫瘍許容的環境に変化させることを示唆しており、糖尿病合併乳癌の治療を考える上で重要な知見が得られたと考えられる。この方法論を駆使し、より多くのサンプルを用いて乳癌の免疫学的微小環境の包括的解析を行い、新規バイオマーカーの探索や治療法の開発につなげたい。