2021 Fiscal Year Final Research Report
Development of Bone Regeneration Methods for Segmental Bone Transport
Project/Area Number |
20K23008
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund |
Review Section |
0906:Surgery related to the biological and sensory functions and related fields
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Research Institution | Kitasato University |
Principal Investigator |
Tazawa Ryo 北里大学, 医学部, 助教 (30749883)
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Project Period (FY) |
2020-09-11 – 2022-03-31
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Keywords | 骨移動術 / 骨形成促進剤 / 成長因子徐放担体 |
Outline of Final Research Achievements |
This study aimed to investigate whether a single injection of BMP-2-loaded in situ-formed hyaluronan gel (IFHG) accelerates consolidation at the bone defect site and bone union at the docking site in a mouse segmental bone transport (SBT) model. A critical sized bone defect was created in the femur of mice and subsequently reconstructed using SBT with an external fixator. Mice were divided into PBS 1.0 (bone segments moved 1.0 mm/day for 2 days) and BMP-2/IFHG (BMP-2/IFHG injected into the bone defect and segments moved 1.0 mm/day for 2 days). In PBS group, maturation of regenerative bone at the bone defect site was poor, with the central area between the proximal and distal bone composed mainly of masses of fibrous and adipose tissue. BMP-2/IFHG groups had higher bone volume and bone mineral content compared to PBS, and all mice achieved bone union at the bone defect and docking sites. BMP-2/IFHG combined with SBT may be effective for enhancing bone healing in large bone defects.
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Free Research Field |
整形外科学
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Academic Significance and Societal Importance of the Research Achievements |
本研究成果は患者のQOL、ADL向上と外傷患者の早期離床、早期社会復帰の実現に大いに貢献できるものと考えられる。
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