2020 Fiscal Year Research-status Report
Periodontal tissue regeneration using a complex cell sheet
| Project/Area Number |
20K23057
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| Research Institution | The University of Tokushima |
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Principal Investigator |
ラジュ レスミ 徳島大学, 大学院医歯薬学研究部(歯学域), 特任研究員 (40876702)
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| Project Period (FY) |
2020-09-11 – 2022-03-31
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| Keywords | Periodontal tissue / regeneration |
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| Outline of Annual Research Achievements |
PDL tissue regeneration has always been a challenge for researchers, as it requires regeneration of triphasic tissue-ligament interface containing both hard (cementum and alveolar bone) and soft (PDL ligament) tissues. The previous research showed that long term tissue responses to the non-living scaffold materials are still questionable and by it may not be possible for complete regeneration of large PDL defects using a single type of cell. The aim of our study was to regenerate a severe PDL defect using a novel type of complex cell sheet (CCS) containing two different types of cells. CCS was fabricated by layering PDL cells (to regenerate cementum and periodontal ligament) and osteogenic differentiated PDL cells (to regenerate bone). Cell sheets were transplanted ectopically (subrenal capsule) and orthotopically (intraoral periodontal injury model) into immunocompromised mice. Ectopic transplants were analyzed following 4 weeks. We could not observe complete regeneration in any groups. Hence, we decided to evaluate it after 8 weeks and found that there was complete regeneration of PDL tissue using CCS. We analyzed using micro-CT, histological staining and immunohistochemistry. Our results show that a CCS can regenerate the PDL tissue similar to is natural anatomy as a multitissue.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have successfully fabricated the CCS using rat PDL cells (to regenerate cementum and periodontal ligament) and osteogenic differentiated PDL cells (to regenerate bone). Furthermore we did ectopic transplantation in the subrenal capsule and analyzed using histology and micro-CT.
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| Strategy for Future Research Activity |
This year study plan is continue the research using orthotopic transplantation of CCS in periodontal tissue injury model mice and analyze the data.
In addition we are planning check the regeneration of tooth dentin-pulp complex (DPC) using the same technique we applied to regenerate the PDL tissue by CCS. Dental pulp stem cells (DPSCs) and Odontogenic differentiated DPSCs will be used to make a CCS. An ectopic (subrenal capsule) and orthotopic (intraoral pulpitis model) transplantation will be carried out in immunocompromised mice.
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| Causes of Carryover |
To buy the culture dishes, experimental animals and reagents necessary for the remaining study.
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Research Products
(1 results)
