2021 Fiscal Year Annual Research Report

Periodontal tissue regeneration using a complex cell sheet

Research Project

Project/Area Number	20K23057
Research Institution	National Center for Geriatrics and Gerontology
Principal Investigator	ラジュレスミ国立研究開発法人国立長寿医療研究センター, ジェロサイエンス研究センター, 研究員 (40876702)
Project Period (FY)	2020-09-11 – 2022-03-31
Keywords	regeneration / tissue engineering / periodontal tissue / tooth regeneration
Outline of Annual Research Achievements	Complex cell sheet, CCS was made using rat PDL cells and osteogenic differentiated PDL cells. Transplants from ectopic transplantation analysis using histology and micro-CT showed the formation of periodontal ligament like bone like tissue. Furthermore, we have successfully created an orthotropic injury mice model for the transplantation of cell sheets. In a continuation study, we have evaluated the regenerative capacity of tooth dentin-pulp tissue by a CCS. Dental pulp stem cells, DPSCs and odontogenic differentiated DPSCs were utilized to fabricate a CCS. Ectopic transplants after 8 weeks of transplantation revealed the formation of dentin-pulp like combined hard and soft tissue formation.