Establishment of a novel tooth regeneration method by controlling extracellular microenvironment using honeycomb TCP

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K23079
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Okayama University
• Principal Investigator: Inada Yasunori 岡山大学, 大学病院, 医員 (50883427)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: ハニカムTCP / 人工生体材料 / 幾何学構造 / 象牙芽細胞株 / 歯牙再生

Outline of Final Research Achievements

In this application project, dentinoblast cell lines established from GFP rats and a novel biomaterial, honeycomb TCP, were used to regenerate teeth with polarity that functions physiologically in vivo. study indicates that 300TCP is an appropriate artificial biomaterial for dentin regeneration, because 300TCP induced TGC to differentiate into polar odontoblast-like hard tissue that is similar to a normal dentin-like structure. Therefore, 300TCP may serve as a new biological material for dentin regeneration and a 300TCP/TGC complex could be used as a model for further studies about dentin regeneration.

Free Research Field

口腔病理学

Academic Significance and Societal Importance of the Research Achievements

現在の歯胚を用いた器官原基法や歯肉から樹立したiPS細胞を用いる方法では歯の再生率が低く、細胞供給源に制約があるなどの問題があり、臨床応用可能な方策は示されておらず、更なる研究の発展や、簡便で確実な歯の再生法の開発が望まれている。本研究で使用した樹立細胞株と生体材料による象牙質再生が可能となれば、数千万人と考えらる患者の潜在的な需要から新規歯牙再生法の大きなニーズを新たに創出できると考えられ、新規歯牙再生法の産業界に対する貢献度は多大であると考えられる。