The establishment of effective oral care using in situ dental biofilm model

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K23104
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Niigata University (2021); Osaka University (2020)
• Principal Investigator: Sotozono Maki 新潟大学, 医歯学総合研究科, 助教 (00876675)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: デンタルバイオフィルム / 睡眠 / 細菌叢

Outline of Final Research Achievements

In this study, the effects of sleep on characteristics of dental biofilm were investigated using in situ dental biofilm model, which enabled us to form experimental dental biofilm in oral cavity, in order to explore effective method of oral care. The viable bacterial cell count, real-time PCR, 16S rRNA sequences analysis and confocal laser scanning microscopy observation were performed.
This study revealed that dental biofilms containing obligate anaerobes were formed and the structures of dental biofilms changed during sleep, whereas the numbers of biofilm-forming bacteria did not change during sleep. The findings of this study will aid in establishment of evidence-based methods for improved oral care.

Free Research Field

細菌学

Academic Significance and Societal Importance of the Research Achievements

う蝕や歯周病の原因となるデンタルバイオフィルム（プラーク）を構成する細菌は就寝中に急激に増加すると予想されてきた。しかし、本研究で就寝中のデンタルバイオフィルム構成細菌数の増加率は起床時と差は認められなかった。一方で、デンタルバイオフィルムを構成している細菌の種類の割合が変化していることが明らかとなった。この結果は、口腔ケアに対して新たな知見を与える可能性がある。