Elucidation of bone marrow formation ability of BMP-2 by photogene manipulation technology

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K23110
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Okayama University
• Principal Investigator: TANAKA YUKIE 岡山大学, 大学病院, 医員 (20884836)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: BMP-2 / 骨髄形成 / 細胞トラッキング技術 / オプトジェネティク

Outline of Final Research Achievements

Hematopoiesis is known to be a major role of bone. However, it is unclear whether BMP-2-induced bone has functions as a hematopoietic organ. In this study, we investigated the details of cellular composition and hematopoietic function of BMP-2-induced bone. Histological and flow cytometric analysis revealed that BMP-2-induced bone had a sinusoidal structure similar to that of the femur and contained not only osteoblasts and osteoclasts but also hematopoietic stem cells and various progenitor cells. In addition, hematopoietic stem cells in BMP-2-induced bone were confirmed to have hematopoietic function by bone marrow transplantation experiments. These results indicate that BMP-2 is a protein that can form bone marrow with physiological functions.

Free Research Field

骨再生

Academic Significance and Societal Importance of the Research Achievements

BMP-2の強力な骨形成能は周知の事実であるが，骨髄形成能という新たな機能を見出し，そのメカニズムを解明する研究は世界的にも皆無であり，本研究は学術的独自性が非常に高いと考える.また，骨髄ニッチ形成細胞の一つであるCAR細胞がMSCとしての機能を持ち合わせており，MSC移植が免疫疾患治療として臨床応用されていることから考えると，CAR細胞の動員・誘導のメカニズムを明らかにすることは，骨免疫学と骨髄生物学を融合した新たなCAR細胞生物学やそれに基づく治療法を創造することに貢献できる.