Study on the expression regulation of platelet-type 12-lipoxygenase in the development of non-alcoholic steatohepatitis.

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K23293
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0909:Sports sciences, physical education, health sciences, and related fields
• Research Institution: Okayama Prefectural University
• Principal Investigator: Toda Keisuke 岡山県立大学, 保健福祉学部, 助教 (80881630)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: 12-リポキシゲナーゼ / 肝星細胞 / 非アルコール性脂肪性肝炎

Outline of Final Research Achievements

In this study, we investigated the regulatory mechanism of the platelet-type 12-lipoxygenase expression. The expression level of platelet-type 12-lipoxygenase on day 1 was significantly reduced compared with that on day 0 in mouse primary hepatic stellate cells. Therefore, it was necessary to find the culture conditions of the cells in which the expression of the enzyme was maintained. The mRNA level of the platelet-type 12-lipoxygenase as examined RT-PCR was not increased by the addition of cytokines associated with progression of liver fibrosis.

Free Research Field

脂質生化学

Academic Significance and Societal Importance of the Research Achievements

今後、血小板型12-リポキシゲナーゼ発現調節機構が明らかになり、NASHにおける肝線維化の機序が解明された場合、アルコール性肝炎やウイルス性肝炎など他の多くの慢性炎症性肝疾患の進展における肝線維化の理解にも応用できる可能性があると考えられ、本研究の学術的・社会的意義は大きい。