2020 Fiscal Year Research-status Report
tRNA epitranscriptome in regulating glioma therapeutic resistance
| Project/Area Number |
20KK0338
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| Research Institution | Tohoku University |
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Principal Investigator |
Rashad Sherif 東北大学, 医学系研究科, 助教 (00824088)
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| Project Period (FY) |
2020 – 2022
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| Keywords | Glioma / tRNA / CRISPR / Chemotherapy / Epigenetics |
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| Outline of Annual Research Achievements |
Studying Glioma epitranscriptome is a novel field of research. Here, I will study the dynamic tRNA epitranscriptome involved in Glioma chemotherapeutic responses to evaluate tRNA dynamics that influence this process and to develop future therapies for Glio ma. Glioma cell lines will be exposed to various chemotherapeutic agents and studied for changes in tRNA levels and modification s using mass spectrometry and sequencing. Moreover, CRISPR screens will be used to identify potential targets for therapy that w ill be explored via genetic approaches.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Experiments are progressing smoothly and initial results shall be available within few months. CRISPR screening is progressing smoothly. Sequencing experiments are also progressing smoothly.
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| Strategy for Future Research Activity |
The next stage will focus on analysis of CRISPR screening results as well as RNA sequencing results to discover novel regulators for ferroptosis and the role that tRNA Biology plays in that process in glioma.
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