Molecular mechanisms that determine kinetochore orientation acting at the heart of genome transition

2013 Fiscal Year Final Research Report

• Project/Area Number: 21000010
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: WATANABE Yoshinori 東京大学, 分子細胞生物学研究所, 教授 (20212326)
• Project Period (FY): 2009 – 2012
• Keywords: 染色体 / セントロメア / 接着因子コヒーシン / シュゴシン / コンデンシン

Research Abstract

We have proven that cohesion at the core centromere defines mono-orientation of kinetochores. We show that chiasmata between homologous chromosomes ensure mono-orientation. We identified the kinase antagonizing shugoshin-PP2A. We further identified the inner centromere-shugoshin (ICS) network that is critical for chromosome segregation and the conserved regulatory mechanism of shaping chromosomes.

Research Products

• [Journal Article] Mps1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components (2012)
  • Author(s): Yamagishi, Y., Yang, CH., Tanno, Y., & Watanabe, Y
  • Journal Title: Nature Cell Biol Volume: 14 Pages: 746-752
  • DOI: doi:10.1038/ncb2515
  • Peer Reviewed
• [Journal Article] Geometry and force behind kinetochore orientation : lessons from meiosis (review article) (2012)
  • Author(s): Watanabe, Y
  • Journal Title: Nature Rev. Mol. Cell Biol Volume: 13 Pages: 370-382
  • Peer Reviewed
• [Journal Article] A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis (2012)
  • Author(s): Morimoto, A., Shibuya, H., Zhu, X., Kim, J., Ishiguro, K., Han M, Watanabe, Y
  • Journal Title: J. Cell Biol Volume: 198 Pages: 165-72
  • DOI: doi:10.1083/jcb.201204085
  • Peer Reviewed
• [Journal Article] Repositioning of Aurora B promoted by chiasmata ensures sister chromatid mono-orientation at meiosis I (2011)
  • Author(s): Sakuno, T., Tanaka, K., Hauf, S., & Watanabe, Y
  • Journal Title: Dev. Cell Volume: 21 Pages: 534-545
  • DOI: doi:10.1016/j.devcel.2011.08.012
  • Peer Reviewed
• [Journal Article] Condensin association with histone H2A shapes mitotic chromosomes (2011)
  • Author(s): Tada, K., Susumu, H., Sakuno, T., & Watanabe, Y
  • Journal Title: Nature (article) Volume: 474 Pages: 477-483
  • DOI: doi:10.1038/nature10179
  • Peer Reviewed
• [Journal Article] Two histone marks establish the inner centromere and chromosome bi-orientation (2010)
  • Author(s): Yamagishi, Y., Honda, T., Tanno, Y., & Watanabe, Y
  • Journal Title: Science Volume: 330 Pages: 239-243
  • DOI: doi:10.1126/science.1194498
  • Peer Reviewed
• [Journal Article] Phosphorylation of the CPC by Cdk1 promotes chromosome bi-orientation (2010)
  • Author(s): Tsukahara, T., Tanno, Y., & Watanabe, Y
  • Journal Title: Nature Volume: 467 Pages: 719-723
  • DOI: doi:10.1038/nature09390
  • Peer Reviewed
• [Journal Article] Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres (2010)
  • Author(s): Tanno Y., Kitajima T.S., Honda, T., Ando Y., Ishiguro K., & Watanabe, Y
  • Journal Title: Genes Dev Volume: 24 Pages: 2169-2179
  • DOI: doi:10.1101/gad.1945310
  • Peer Reviewed
• [Journal Article] Shugoshin-PP2A counteracts Casein Kinase 1-dependent cleavage of Rec8 by separase (2010)
  • Author(s): Ishiguro, T., Tanaka, K., Sakuno, T., & Watanabe, Y
  • Journal Title: Nature Cell Biol Volume: 12 Pages: 500-506
  • DOI: doi:10.1038/ncb2052
  • Peer Reviewed
• [Journal Article] Phosphorylation of H2A by Bub1 prevents chromosomal instability through localizing shugoshin (2010)
  • Author(s): Kawashima, S.A., Yamagishi, Y., Honda, T., Ishiguro, K., & Watanabe, Y
  • Journal Title: Science(article) Volume: 327 Pages: 172-177
  • DOI: doi:10.1126/science.1180189
  • Peer Reviewed
• [Journal Article] CENP-C functions as a scaffold for effectors with essential kinetochore functions in mitosis and meiosis (2009)
  • Author(s): Tanaka, K., Chang, H.L., Kagami, A., & Watanabe, Y
  • Journal Title: Dev. Cell Volume: 17 Pages: 334-343
  • DOI: doi:10.1016/j.devcel.2009.08.004
  • Peer Reviewed
• [Journal Article] Kinetochore geometry defined by cohesion within the centromere (2009)
  • Author(s): Sakuno, T., Tada, K., & Watanabe, Y
  • Journal Title: Nature(article) Volume: 458 Pages: 852-858
  • DOI: doi:10.1038/nature07876
  • Peer Reviewed
• [Presentation] The spindle assembly checkpoint (SAC) network links directly to chromosome alignment (2013)
  • Author(s): Watanabe, Y
  • Organizer: Cell Biology of Yeasts
  • Place of Presentation: New York, USA
  • Year and Date: 2013-11-08
• [Presentation] Cohesin Rad21L mediates DSB-independent homolog pairing in mouse spermatocytes (2013)
  • Author(s): Watanabe, Y
  • Organizer: EMBO Workshop on : Meiosis
  • Place of Presentation: Dresden, Germany
  • Year and Date: 2013-10-18
• [Presentation] The major mechanism of chromosomal instability in cancer cells (2013)
  • Author(s): Watanabe, Y
  • Organizer: Wenner-Gren Foundations International Symposium, Meiosis and chromosome segregation - a mammalian perspective
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2013-08-30
• [Presentation] Tension across centromeres refines centromeric protection by shugoshin (2012)
  • Author(s): Watanabe, Y
  • Organizer: EMBO Workshop on : Structure, Function & Regulation of Centromeres and Kinetochores
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2012-10-03
• [Presentation] Tension across centromeres changes centromeric protection by shugoshin (2012)
  • Author(s): Watanabe, Y
  • Organizer: FASEB Mitosis : Spindle Assembly and Function
  • Place of Presentation: Colorado, USA
  • Year and Date: 2012-08-08
• [Presentation] Chromosome segregation in meiosis (2012)
  • Author(s): Watanabe, Y
  • Organizer: FASEB Yeast Chromosome Structure, Replication & Segregation
  • Place of Presentation: Colorado, USA
  • Year and Date: 2012-07-18
• [Presentation] Pairing might be promoted by identical cohesin distribution patterns between homologs (2012)
  • Author(s): Watanabe, Y
  • Organizer: Gordon Research Seminar : The 2012 Meiosis Gordon Research Conference
  • Place of Presentation: New London, USA
  • Year and Date: 2012-06-06
• [Presentation] Histone marks for shugoshin-CPC localization prevent chromosomal instability (2012)
  • Author(s): Watanabe, Y
  • Organizer: Epigenetics meeting
  • Place of Presentation: Adelaide, AUS
  • Year and Date: 2012-05-08
• [Presentation] Aurora B-dependent condensin association with histone H2A shapes mitotic chromosomes (2011)
  • Author(s): Watanabe, Y
  • Organizer: Sixth international Fission Yeast Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2011-06-27
• [Presentation] Spatiotemporal regulation of chromosome architecture by Aurora B (2011)
  • Author(s): Watanabe, Y
  • Organizer: The 6th UK-Japan Cell Cycle Workshop
  • Place of Presentation: Windermere, U. K.
  • Year and Date: 2011-04-12
• [Presentation] Shugoshin impacting on eukaryotic chromosome segregation (2010)
  • Author(s): Watanabe, Y
  • Organizer: Integrated aspects of molecular cell biology
  • Place of Presentation: Lion, France
  • Year and Date: 2010-11-09
• [Presentation] Interplay of kinetochore geometry and tension determines chromosome orientation during mitosis and meiosis (2010)
  • Author(s): Watanabe, Y
  • Organizer: FASEB Summer Research Conference
  • Place of Presentation: Arizona, USA
  • Year and Date: 2010-08-10
• [Presentation] Interplay of kinetochore geometry and tension determines chromosome orientation during meiosis (2010)
  • Author(s): Watanabe, Y., Tanaka, K., Sakuno, T
  • Organizer: Gordon Research Conference on Meiosis
  • Place of Presentation: New Hampshire, USA
  • Year and Date: 2010-06-10
• [Presentation] Phosphorylation of H3 and H2A establishes the inner centromere and chromosome bi-orientation (2010)
  • Author(s): Watanabe, Y
  • Organizer: The LXXV Cold Spring Harbor Symposium on Quantitative Biology Nuclear Organization & Function
  • Place of Presentation: New York, USA
  • Year and Date: 2010-06-07
• [Presentation] Regulation of centromeric localization of shugoshin (2009)
  • Author(s): Watanabe, Y
  • Organizer: The EMBO conference on meiosis (1^<st>)
  • Place of Presentation: Sorgue, France
  • Year and Date: 2009-09-22
• [Presentation] Identification of the primary Bub1 substrate impacting on chromosome segregation (2009)
  • Author(s): Watanabe, Y
  • Organizer: Late summer meeting (Chromosome Dynamics)
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2009-09-10
• [Presentation] Aurora B mediated phosphorylation of hSgo2 is crucial for chromosome congression and centromeric protection (2009)
  • Author(s): Watanabe, Y
  • Organizer: International Workshop on Chromosome Segregation Machinery
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-06-05
• [Presentation] Kinetochore orientation is elaborately regulated by cohesion within the centromere (2009)
  • Author(s): Watanabe, Y
  • Organizer: Gordon Research Conference on Chromosome Dynamics
  • Place of Presentation: Il Ciocco Hotel and Resort Barga, Italy
  • Year and Date: 2009-05-27
• [Remarks] 1. 生殖細胞への関与物質特定(日経産業新聞 2014.3.11)
• [Remarks] 2. 染色体分配の基本原理の解明(読売新聞 2013.3.21)
• [Remarks] 3. 染色体分配の基本原理の解明(朝日新聞 2013.3.21)
• [Remarks] 4. 染色体の分配制御仕組みを発見(毎日新聞 2009.12.1)
• [Remarks]
  • URL: http://www.iam.u-tokyo.ac.jp/watanabe-lab/watanabe_lab/Home.html