Establishment of a method for restoring vision using light-gated ion channels and the promotion of optogenetics

2011 Fiscal Year Final Research Report

• Project/Area Number: 21200022
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology; Biomedical engineering/Biological material science
• Research Institution: Tohoku University
• Principal Investigator: TAMAI Makoto 東北大学, 大学院・医学系研究科, 名誉教授 (90004720)
• Co-Investigator(Kenkyū-buntansha): IMAMURA Kazuyuki 前橋工科大学, システム生体工学科, 教授 (30203326) ; SUGANO Eriko 東北大学, 国際高等融合領域研究所, 助教 (70375210) ; ISAGO Hitomi 東北大学, 国際高等融合領域研究所, 技術補佐員 (30400451)
• Co-Investigator(Renkei-kenkyūsha): TOMITA Hiroshi 東北大学, 国際高等融合領域研究所, 准教授 (40302088) ; WATANABE Masami 東北大学, 国際高等融合領域研究所, 非常勤講師 (10093486)
• Project Period (FY): 2009 – 2011
• Keywords: 光遺伝学 / 視覚再生 / 網膜

Research Abstract

We have newly developed a light gated cation channel gene by the modification of channelrhodpsin gene derived from the clamydomonas. The modified channelrhodopsin has a sensitivity covered over a visible light. We could regain the visual function of blind patients by using the modified channelrhodopsin. Indeed, we could demonstrate the recovery of visual function in genetically blind rats by transucing the gene.

Research Products

• [Journal Article] Age-dependent differences in recovered visual responses in Royal College of Surgeons rats transduced with the Channelrhodopsin-2 gene. (2012)
  • Author(s): Isago H, Sugano E, Wang Z, Murayama N, Tamai M, Tomita H.
  • Journal Title: J Mol Neurosci Volume: 46(2) Pages: 393-400
  • Peer Reviewed
• [Journal Article] Differentiation of neuronal cells from NIH/3T3 fibroblasts under defined conditions. (2011)
  • Author(s): Wang Z, Sugano E, Isago H, Hiroi T, Tamai M, Tomita H.
  • Journal Title: Development Growth and Differentiation Volume: 53(3) Pages: 357-65
  • Peer Reviewed
• [Journal Article] Immune responses to adeno-associated virus type 2 encoding channelrhodopsin-2 in a genetically blind rat model for gene therapy. (2011)
  • Author(s): Sugano E, Isago H, Wang Z, Murayama N, Tamai M, Tomita H.
  • Journal Title: Gene Therapy Volume: 18(3) Pages: 266-74
  • Peer Reviewed
• [Journal Article] Dissecting a Role for Melanopsin in Behavioural Light Aversion Reveals a Response Independent. (2010)
  • Author(s): Semo M, Gias C, Ahmado A, Sugano E, Allen A, Lawrence JM, Tomita H, Coffey PJ, Vugler A^*.
  • Journal Title: PLoS ONE Volume: 29; 5(11) Pages: e15009
  • Peer Reviewed
• [Journal Article] Channelrhodopsin-2 gene transduced into retinal ganglion cells restores functional vision in genetically blind rats. (2010)
  • Author(s): Tomita H, Sugano E, Isago H, Hiroi T, Wang Z, Ohta E, Tamai M.
  • Journal Title: Exp Eye Res Volume: 90 Pages: 426-436
  • Peer Reviewed
• [Journal Article] Involvement of endplasmic reticulum stress on neuronal cell death in the lateral geniculate nucleus in the monkey glaucoma model (2010)
  • Author(s): Ito Y, Shimazawa M, Inokuchi Y, Yamanaka H, Tsuruma K, Imamura K, Onoe H, Watanabe Y, Aihara M, Arae M, Hara H.
  • Volume: 33
  • Pages: 843-55
  • Peer Reviewed
• [Journal Article] Cortical activation during color discrimination task in macaques as revealed by positron emission tomography. (2010)
  • Author(s): Katsuyama N, Imamura K, Onoe H, Tanaka H.K, Onoe K, Tsukada H, Watanabe Y.
  • Journal Title: Neurosci Letter Volume: 484(3) Pages: 168-73
  • Peer Reviewed
• [Journal Article] Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the Thy-1.2 promoter. (2009)
  • Author(s): Tomita H, Sugano E, Fukazawa Y, Isago H, Sugiyama Y, Hiroi T, Ishizuka T, Mushiake H, Kato M, Hirabayashi M, Shigemoto R, Yawo H, Tamai M.
  • Journal Title: PLoS ONE Volume: 4(11) Pages: e7679
  • Peer Reviewed
• [Journal Article] Channelrhodopsins provide a breakthrough insight into strategies for curing blindness. (2009)
  • Author(s): Tomita H, Sugano E, Isago H, Tamai M.
  • Journal Title: J Genetics Volume: 88 Pages: 409-415
  • Peer Reviewed
• [Journal Article] Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. (2009)
  • Author(s): Wang H, Sugiyama Y, Hikima T, Sugano E, Tomita H, Takahashi T, Ishizuka T, Yawo H.
  • Journal Title: J Biol Chem Volume: 284 Pages: 5685-5696
  • Peer Reviewed
• [Journal Article] Molecular imaging reveals unique degenerative changes in experimental glaucoma. (2009)
  • Author(s): Imamura K, Onoe H, Shimazawa M, Nozaki S, Wada Y, Kato K, Nakajima H, Mizuma H, Onoe K, Taniguchi T, Sasaoka M, Hara H, Tanaka S, Araie M, Watanabe Y.
  • Journal Title: Neuroreport Volume: 20(2) Pages: 139-144
  • Peer Reviewed
• [Journal Article] A postnatal critical period for orientation plasticity in the cat visual cortex. (2009)
  • Author(s): Tanaka S, Tani T, Ribot J, O’hashi K, Imamura K.
  • Volume: 4(4)
  • Pages: e5380
  • Peer Reviewed
• [Presentation] Induction of neuronal cells from NIH/3T3 fibroblasts under defined conditions (2011)
  • Author(s): Wang Z, Sugano E, Tomita H
  • Organizer: The 5th China Medicinal Biotech Forum（CMBF2011)
  • Place of Presentation: 北京
  • Year and Date: 2011-11-08
• [Presentation] チャネルロドプシン-2を用いた視覚再生の可能性 (2011)
  • Author(s): 菅野江里子、砂金ひとみ、富田浩史
  • Organizer: 第6回網脈絡膜変性フォーラム
  • Place of Presentation: 盛岡
  • Year and Date: 2011-10-10
• [Presentation] ボルボックス由来チャネルロドプシン1遺伝子改変による感受波長の改善 (2011)
  • Author(s): 菅野江里子、砂金ひとみ、村山なみえ、玉井信、富田浩史
  • Organizer: 第82回日本動物学会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] 視細胞変性モデルの確立 (2011)
  • Author(s): 富田浩史、菅野江里子、砂金ひとみ、村山なみえ、玉井信
  • Organizer: 第82回日本動物学会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] チャネルロドプシン2を導入した週齢の異なるRCSラットの視覚反応の違い (2011)
  • Author(s): 砂金ひとみ、菅野江里子、村山なみえ、玉井信、富田浩史
  • Organizer: 第82回日本動物学会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] Gene therapy in opjtjalmology `Visual properties of genetically blind rats transduced with channelrhodopsin-2 gene." (2011)
  • Author(s): Tomita H, Sugano E, Isago H, Murayama N, Wang Z, Tamai M
  • Organizer: The Fourth Global Chinese Ophthalmic conference, The 16th Congress of chinese Ophthalmological Society
  • Place of Presentation: 広州（中国）
  • Year and Date: 2011-09-08
• [Presentation] チャネルロドプシン2による視覚再生治療における効果的時期の検討 (2011)
  • Author(s): 菅野江里子
  • Organizer: 「光感受性分子の遺伝子導入による新たな視覚系構築ち再生医工学研究領域の創成」研究班会議
  • Place of Presentation: 群馬
  • Year and Date: 2011-07-16
• [Presentation] 視機能再建のための遺伝子治療～実現に向けて (2011)
  • Author(s): 富田浩史、菅野江里子、砂金ひとみ
  • Organizer: JRPS三重県支部医療講演会
  • Place of Presentation: 松阪市
  • Year and Date: 2011-06-26
• [Presentation] 藻類チャネルロドプシン遺伝子を用いた視覚再生 (2011)
  • Author(s): 菅野江里子、砂金ひとみ、富田浩史
  • Organizer: JRPS大阪支部医療講演会
  • Place of Presentation: 大阪市
  • Year and Date: 2011-06-11
• [Presentation] 能再生に向けた遺伝子遺伝子治療研究の現状 (2011)
  • Author(s): 富田浩史、菅野江里子、砂金ひとみ
  • Organizer: 第12回網膜色素変性症患者のつどい
  • Place of Presentation: 福岡市
  • Year and Date: 2011-06-05
• [Presentation] チャネルロドプシン2を用いた視覚の再生について (2011)
  • Author(s): 菅野江里子、砂金ひとみ、玉井信、富田浩史
  • Organizer: JRPS（網膜色素変性症協会）新潟支部医療講演会
  • Place of Presentation: 新潟市
  • Year and Date: 2011-05-22
• [Presentation] 視覚再生研究の新たな展開 (2011)
  • Author(s): 富田浩史、菅野江里子、砂金ひとみ
  • Organizer: 日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-12
• [Presentation] Effective Time Point For Channelrhodopsin-2 mediated Gene Therapy After Photoreceptor Degeneration In Rcs Rats (2011)
  • Author(s): Sugano E, Isago H, Wang Z, Murayama N, Tamai M, Tomita H
  • Organizer: ARVO Annual meeting
  • Place of Presentation: Fortlauderdale, FL
  • Year and Date: 2011-05-01
• [Presentation] mprovement Of Wavelength Sensitivities By The Modification Of Volvox Channelrhodopsin-1 Gene (2011)
  • Author(s): Tomita H, Sugano E, Isago H, Murayama N, Wang Z, Tamai M
  • Organizer: ARVO Annual meeting
  • Place of Presentation: Fortlauderdale, FL
  • Year and Date: 2011-05-01
• [Presentation] Invovement of drebrin, an actin binding protein, in regulation of AMPA receptor recruitment in cultured hippocampal neurons (2011)
  • Author(s): Kato K, Imamura K, Sekino Y
  • Organizer: International radiation neurobiology
  • Place of Presentation: 群馬
  • Year and Date: 2011-01-29
• [Presentation] Ocular dominance plasticity in abnormally developed visual cortex (2011)
  • Author(s): Imamura K
  • Organizer: International radiation neurobiology
  • Place of Presentation: 群馬
  • Year and Date: 2011-01-29
• [Presentation] 遺伝子導入による視覚再建 (2011)
  • Author(s): 富田浩史, 菅野江里子, 砂金ひとみ, 王卓, 村山なみえ, 玉井信
  • Organizer: 日本視覚学会
  • Place of Presentation: 東京
  • Year and Date: 2011-01-20
• [Presentation] Retinal and systemic immune responses after the transfer of channelrhodopsin-2 into RCS rats (2010)
  • Author(s): Tomita, H., Sugano, E., Isago, H., Hiroi, T., Wang, Z. and Tamai, M
  • Organizer: XIVth International Symposium on Retinal Degeneration
  • Place of Presentation: Quebec, Canada
  • Year and Date: 20100714-16
• [Presentation] Optimization of volvox-derived channelrhodopsin-1 to expressexpression in mammalian cells (2010)
  • Author(s): Sugano, E., Tomita, H., Isago, H., Hiroi, T., Wang, Z. and Tamai, M
  • Organizer: XIVth International Symposium on Retinal Degeneration
  • Place of Presentation: Quebec, Canada
  • Year and Date: 20100714-16
• [Presentation] 藻類由来遺伝子を用いた視覚再生技術 (2010)
  • Author(s): 富田浩史, 菅野江里子, 砂金ひとみ, 王卓, 村山なみえ, 玉井信
  • Organizer: 日本人工臓器学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-11-20
• [Presentation] Visual Responses of Royal College of Surgeons Rats Transferred Modified Volvox Channelrhodopsin-2 Gene. (2010)
  • Author(s): Tomita, H., Sugano, E., Isago, H., Hiroi, T., Wang, Z. and Tamai, M
  • Organizer: nnual meeting Association for Research in Vision and Ophthalmology
  • Place of Presentation: Ft. Lauderdale, US
  • Year and Date: 2010-05-02
• [Presentation] Systematic and Local Responses of Channelrhodopsin-2 Gene Therapy (2010)
  • Author(s): Sugano, E., Tomita, H., Isago, H., Hiroi, T., Wang, Z. and Tamai, M
  • Organizer: Annual meeting Association for Research in Vision and Ophthalmology
  • Place of Presentation: Ft. Lauderdale, US
  • Year and Date: 2010-05-02
• [Presentation] カニクイザルを用いた視機能評価 (2009)
  • Author(s): 菅野江里子、富田浩史、姫志剛、砂金ひとみ、廣井照、松坂昌哉、虫明元、玉井信
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-19
• [Presentation] Channelrhodopsin-2トランスジェニックラットの視機能について (2009)
  • Author(s): 砂金ひとみ, 富田浩史, 菅野江里子, 廣井照, 王卓, 玉井信
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-19
• [Presentation] EMG and motor recovery (2009)
  • Author(s): Nishino K, Imamura K
  • Organizer: XIV World Congress of Neurological Surgery of the World Federation of Neurosurgical Societies
  • Place of Presentation: Boston, MA, US
  • Year and Date: 2009-09-02
• [Presentation] Central changes in Experimental Glaucoma (2009)
  • Author(s): Imamura K, Onoe H, Shimazawa M, Nozaki S, Mizuma H, Wada Y, Onoe K, Ishii K, Mayama C, Taniguchi T, Sasaoka M, Yamanaka H, Hara H, Tanaka S, Araie M, Watanabe Y
  • Organizer: 国際生理学会世界大会
  • Place of Presentation: 京都
  • Year and Date: 2009-08-01
• [Book] チャネルロドプシンを用いた視覚再生、日本の眼科、82：12 号 (2011)
  • Author(s): 富田浩史、菅野江里子
  • Total Pages: 1602-1607
  • Publisher: 社団法人日本眼科医会
• [Remarks]
  • URL: http://www.visual-neuroscience.med.tohoku.ac.jp/index.html
• [Patent(Industrial Property Rights)] 発現効率が改善された光受容チャネルロドプシン (2010)
  • Inventor(s): 富田浩史、菅野江里子
  • Industrial Property Rights Holder: 東北大学
  • Industrial Property Number: PCT/JP2010/063786
  • Filing Date: 2010-07-10
  • Overseas
• [Patent(Industrial Property Rights)] 発現効率が改善された光受容チャネルロドプシン (2009)
  • Inventor(s): 富田浩史、菅野江里子
  • Industrial Property Rights Holder: 東北大学
  • Industrial Property Number: 特願 2009-185455
  • Filing Date: 2009-08-10