Analysis of propagation manners of paramyxoviruses in neural cells.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21200036
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Virology; Nanomaterials/Nanobioscience
• Research Institution: The University of Tokyo
• Principal Investigator: YONEDA Misako 東京大学, 医科学研究所, 准教授 (40361620)
• Co-Investigator(Kenkyū-buntansha): FUJIYUKI Tomoko 東京大学, 医科学研究所, 特任研究員 (50610630) ; OMI Mio 東京大学, 医科学研究所, 特任研究員 (00526767)
• Co-Investigator(Renkei-kenkyūsha): YASUDA Kenji 東京医科歯科大学, 生体材料工学研究所, 教授 (20313158)
• Project Period (FY): 2009 – 2011
• Keywords: 細胞

Research Abstract

Both Measles and Nipah virus causes severe acute or late-onset encephalitis in human. In this study, we aimed to investigate the mechanisms of measles and nipah virus spread between neural cells. We established the AMCA (on-chip agarose microchamber array) system for primary neural cells, and elucidated the propagation manner of MV to neighboring cells. By using fluorescent labeled- viral protein, a new domain, which was important for interaction of NiV N and P protein, was identified. Further, we investigated NiV spread in African green monkey by using EGFP-expressing recombinant NiV. The results indicated that lethal infection to CNS occurred after systemic infection.

Research Products

• [Journal Article] The nucleocapsid protein of measles virus blocks host interferon response (2012)
  • Author(s): Takayama, I., Sato, H., Watanabe, A., Omi-Furutani, M., Kanki, K., Yoneda, M. and Kai, C
  • Journal Title: Virology Volume: 424 Pages: 45-55
• [Journal Article] Measles virus selectively blind to signaling lymphocyte activation molecule as a novel oncolytic virus for breast cancer treatment (2012)
  • Author(s): Sugiyama, T., Yoneda, M., Kuraishi, T., Hattori, S., Inoue, Y., Sato, H. and Kai, C
  • Journal Title: Gene Therapy Pages: 1-10
• [Journal Article] Phophorylation of measles virus phosphoprotein at S86 and/or S151 downregulates viral transcriptional activity (2012)
  • Author(s): Sugai, A., Sato, H., Yoneda, M. and Kai, C
  • Journal Title: FEBS Letters Volume: 586(21) Pages: 3900-7
• [Journal Article] Determination of phosphorylation site in Nipah virus nucleoprotein and its involvement in viral transcription (2011)
  • Author(s): Huang M., Sato H., Hagiwara K., Watanabe A., Sugai A., Ikeda F., Kozuka-Hata H., Oyama M., Yoneda, M. and Kai, C
  • Journal Title: J. Gen. Virol Volume: 92(Pt9) Pages: 2133-2141
• [Journal Article] Comparative and mutational analyses of promoter regions of rinderpest virus (2010)
  • Author(s): Imai, C., Fujita, K., Shimizu, F., Sugai, A., 1Yoneda, M. and Kai, C
  • Journal Title: Virology Volume: 396 Pages: 169-177
• [Journal Article] CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells (2010)
  • Author(s): Watanabe, A., Yoneda, M., Ikeda, F., Terao-Muto, Y., Sato, H., Kai, C
  • Journal Title: J. Virol Volume: 84 (9) Pages: 4183-4193
• [Journal Article] The nonstructural proteins of Nipah virus play a key role in pathogenicity in vivo (2010)
  • Author(s): Yoneda, M., Guillaume, V., Sato, H., Fujita, K., Georges-Courbot, M-C., Ikeda, F., Omi, M., Muto-Terao, Y., Wild, F. and Kai, C
  • Journal Title: PLoS ONE Volume: 5(9) Pages: e12709(1-8)
• [Journal Article] Peroxiredoxin 1 is Required for the efficient transcription and replication of measles virus (2010)
  • Author(s): Watanabe, A., Yoneda, M., Ikeda, F., Sugai, A., Sato, H. and Kai, C
  • Journal Title: J. Virol Volume: 85(5) Pages: 2247-2253
• [Journal Article] Novel phosphoprotein-interacting region in Nipah virus nucleocapsid protein and its involvement in viral replication
  • Author(s): Omi-Furutani, M., Yoneda, M., Fujita, K., Ikeda, F. and Kai, C
  • Journal Title: J. Virol Volume: 84(19) Pages: 9793-9799
• [Presentation] Safe and highly effective vaccine for pig Nipah virus infection using recombinant pseudorabies virus (2011)
  • Author(s): Yoneda, M., Mogi, R., Soda, D., Ikeda, F., Ishii, M., Kawaguchi, Y., Sato, H. and Kai, C
  • Organizer: XVth Int. Cong. Virol
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20110911-16
• [Presentation] The role of Nipah virus accessory proteins in it's pathogenicity in vivo (2010)
  • Author(s): Yoneda, M., Guillaume, V., Sato, H., Fujita, K., Omi, M., Geroges-Courbot, M-C. Ikeda, F., Wild, F. and Kai, C
  • Organizer: XIVth Int. Conf. on Negative Strand Viruses
  • Place of Presentation: Bruges
  • Year and Date: 20100621-25
• [Presentation] Identification of novel N-P interacting domain in Nipah virus nucleocapsid protein (2010)
  • Author(s): Omi, M., Yoneda, M., Fujita, K., Ikeda, F. and Kai, C
  • Organizer: XIVth Int. Conf. on Negative Strand Viruses
  • Place of Presentation: Bruges
  • Year and Date: 20100621-25
• [Presentation] (2010)
  • Author(s): Huang, M., Sato, H. Hagiwara, K., Watanabe, A., Ikeda, F., Oyama, M., Yoneda, M. and Kai, C
  • Organizer: Analysis of phosphorylation residues on Nipah virus nucleoprotein role of the phosphorylation
  • Place of Presentation: Florida, USA
  • Year and Date: 20100309-12
• [Presentation] 蛍光融合蛋白を用いたニパウイルスN蛋白のP蛋白相互作用領域の解明 (2009)
  • Author(s): 小見-古谷美央、米田美佐子、池田房子、甲斐知惠子
  • Organizer: 第56回日本ウイルス学会
  • Place of Presentation: 岡山
  • Year and Date: 20091025-27
• [Presentation] ニパウイルスアクセサリー蛋白の病原性への関与 (2009)
  • Author(s): 米田美佐子、佐藤宏樹、藤田賢太郎、寺尾由里、池田房子、小見美央、甲斐知恵子
  • Organizer: 第56回日本ウイルス学会
  • Place of Presentation: 東京
  • Year and Date: 20091000
• [Book] Journal of Disaster Reseach (2011)
  • Author(s): Kai, C. and Yoneda, M
  • Total Pages: 6, 390-397
  • Publisher: Henipavirus infections - An expanding zoonosis from fruit bats
• [Book] ニパウイルス,ヘンドラウイルス(特集:種の壁を越える感染症-EpidemiologyとEpizootiology-)、臨床と微生物 (2010)
  • Author(s): 米田美佐子、甲斐知恵子
  • Total Pages: 近代出版 37(2):133-138
  • Publisher: 近代出版