Quantitative characterization and molecular mechanism of endoplasmic reticulum dynamics

2011 Fiscal Year Final Research Report

• Project/Area Number: 21200065
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology; Cell biology
• Research Institution: Kyoto University
• Principal Investigator: UEDA Haruko 京都大学, 大学院・理学研究科, 特定准教授 (90402776)
• Co-Investigator(Kenkyū-buntansha): YOKOTA Etsuo 兵庫県立大学, 大学院・生命理学研究科, 助教 (80212299)
• Project Period (FY): 2009 – 2011
• Keywords: 原形質流動 / 小胞体 / アクチン / ミオシン / オルガネラ

Research Abstract

Plants exhibit an ultimate case of the intracellular motility involving rapid organelle trafficking and continuous streaming of the endoplasmic reticulum (ER). To comprehensively analyze the ER movement, we developed software to generate a detailed velocity-distribution map. Streaming pattern of cytosolic GFP was similar to that of the ER-localized GFP, suggesting that a correlation and a causative relationship between the streaming of ER and cytosol. Furthermore, we elucidated a part of the molecular mechanisms of the ER streaming by analyzing ER-associated proteins responsible for ER streaming.

Research Products

• [Journal Article] Myosin XI-dependent formation of tubular structures from endoplasmic reticulum isolated from tobacco cultured BY-2 cells (2011)
  • Author(s): Yokota, E., Ueda, H., Hashimoto, K., Orii, H., Shimada, T., Hara-Nishimura, I., and Shimmen, T.
  • Journal Title: Plant Physiol. Volume: 156 Pages: 129-143
  • DOI: DOI:10. 1104/ pp. 111. 175018
  • Peer Reviewed
• [Journal Article] The myosin ATPase inhibitor, 2,3-butanedione 2-monoxime, prevents protein secretion by the basidiomycete Coprinopsis cinerea (2011)
  • Author(s): Hashimoto, K., Yokota, E., Shimmen, T., and Yoshida, M.
  • Journal Title: Biotechnol. Lett. Volume: 33 Pages: 769-775
  • DOI: DOI:10.1007/s10529-010-0497-0
  • Peer Reviewed
• [Journal Article] Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development (2010)
  • Author(s): Shirakawa, M., Ueda, H., Shimada, T., Koumoto, Y., Shimada, T. L., Kondo, M., Takahashi, T., Okuyama, Y., Nishimura, M., and Hara-Nishimura, I.
  • Journal Title: Plant J. Volume: 64 Pages: 924-935
  • DOI: DOI:10.1111/j.1365-313X.2010.04394.x
  • Peer Reviewed
• [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells (2010)
  • Author(s): Ueda, H., Yokota, E., Kutsuna, N., Shimada, T., Tamura, K., Shimmen, T., Hasezawa, S., Dolja, V. V., and Hara-Nishimura, I.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Pages: 6894-6899
  • DOI: DOI:10.1073/pnas.0911482107
  • Peer Reviewed
• [Journal Article] Vacuolar SNAREs function in the formation of the leaf vascular network by regulating auxin distribution (2009)
  • Author(s): Shirakawa, M.*, Ueda, H.*, Shimada, T., Nishiyama, C., and Hara-Nishimura, I.
  • Journal Title: Plant Cell Physiol Volume: 50 Pages: 1319-1328
  • DOI: DOI:10.1093/pcp/pcp076
  • Peer Reviewed
• [Journal Article] GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 are required for maintenance of endoplasmic reticulum morphology in Arabidopsis thaliana (2009)
  • Author(s): Nakano, R. T., Matsushima, R., Ueda, H., Tamura, K., Shimada, T., Li, L., Hayashi, Y., Kondo, M., Nishimura, M., and Hara-Nishimura, I.
  • Journal Title: Plant Cell Volume: 21 Pages: 3672-3685
  • DOI: DOI:10.1105/tpc.109.068270
  • Peer Reviewed
• [Journal Article] The putative RNA-processing protein, THO2, is a microtubule-associated protein in tobacco (2009)
  • Author(s): Hamada, T., Igarashi, H., Taguchi, R., Fujiwara, M., Fukao, Y., Shimmen, T., Yokota, E., and Sonobe. S.
  • Journal Title: Plant Cell Physiol Volume: 50 Pages: 801-811
  • DOI: DOI:10.1093/pcp/pcp024
  • Peer Reviewed
• [Journal Article] An isoform of myosin XI is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells (2009)
  • Author(s): Yokota, E., Ueda, S., Tamura, K., Orii, H., Uchi, S., Sonobe, S., Hara-Nishimura, I., and Shimmen, T.
  • Journal Title: J. Exp. Bot. Volume: 60 Pages: 197-212
  • DOI: DOI:10.1093/jxp/ern280
  • Peer Reviewed
• [Journal Article] The preprophase band is a localized center of clathrin-mediated endocytosis in late prophase cells of the onion cotyledon epidermis (2009)
  • Author(s): Karahara, I., Suda, J., Tahara, H., Yokota, E., Shimmen, T., Misaki, K., Yonemura, S., Murata, T., Staehelin, L. A., and Mineyuki, Y.
  • Journal Title: Plant J. Volume: 57 Pages: 819-831
  • DOI: DOI:10.1111/j.1365-318X.2008.03725.x
  • Peer Reviewed
• [Presentation] 植物細胞における小胞体ダイナミクス (2012)
  • Author(s): 上田晴子・他
  • Organizer: 第14回オルガネラワークショップ
  • Place of Presentation: 京都大学
  • Year and Date: 2012-03-15
• [Presentation] 小胞体流動に異常を示す変異体の解析 (2011)
  • Author(s): 上田晴子・他
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 20110320-22
• [Presentation] 植物細胞におけるミオシン依存的な小胞体流動とアクチン繊維束の組織化 (2011)
  • Author(s): 上田晴子・他
  • Organizer: 第84回日本生化学会大会(シンポジウム : アクチンとミオシンの意外なしくみとはたらき)
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-21
• [Presentation] Myosin XI drives endoplasmic reticulum motility and organizes F-actin orientations (2011)
  • Author(s): Ueda, H. et al.
  • Organizer: 18^<th> International Botanical Congress (Symposium: Cellular Dynamics)
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2011-07-26
• [Presentation] ER motility and F-actin organization mediated by plant-specific myosins (2010)
  • Author(s): Ueda, H. et al.
  • Organizer: The 2010 Cold Spring Harbor Asia Conference
  • Place of Presentation: Suzhou, China
  • Year and Date: 20101025-29
• [Presentation] ER motility and F-actin organization mediated by plant-specific myosins (2010)
  • Author(s): Ueda, H. et al.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-10
• [Presentation] 植物細胞における小胞体流動メカニズムとその意義 (2010)
  • Author(s): 上田晴子・他
  • Organizer: 日本植物学会近畿支部大会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-11-20
• [Presentation] 植物細胞における小胞体流動メカニズムとその意義 (2010)
  • Author(s): 上田晴子・他
  • Organizer: 第74回日本植物学会大会(シンポジウム : 画像情報から植物を定量的に捉える)
  • Place of Presentation: 中部大学(春日井市)
  • Year and Date: 2010-09-09
• [Presentation] 植物細胞における小胞体流動～定量解析と生理学的意義～ (2010)
  • Author(s): 上田晴子・他
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Remarks]
  • URL: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2009/100323_1.htm