Molecular Anatomy of Synaptic Structure

2013 Fiscal Year Final Research Report

• Project/Area Number: 21220008
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: The University of Tokyo
• Principal Investigator: OKABE Shigeo 東京大学, 医学(系)研究科(研究院), 教授 (60204012)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Shinji 東京大学, 大学院医学系研究科, 助教 (00529034) ; IWASAKI Hirohide 東京大学, 大学院医学系研究科, 講師 (30342752) ; KONDO Satoru 東京大学, 大学院医学系研究科, 講師 (20301757) ; NISHII Kiyomasa 東京大学, 大学院医学系研究科, 助教 (20264020)
• Project Period (FY): 2009-05-11 – 2014-03-31
• Keywords: 分子神経生物学 / シナプス後肥厚部 / 超解像顕微鏡 / 二光子顕微鏡 / シナプス可塑性

Research Abstract

PALM and STORM are superresolution imaging techniques based on detection of single fluorescent molecules. Although postsynaptic densities (PSDs) are disc-like microstructures with their diameters of less than 500 nm, PALM and STORM are able to resolve their internal structure. In this study, fluorescently labeled PSDs and spines were visualized by using PALM and the molecular distributions within these structures were detected. Furthermore, we could successfully detected distributional changes of PSD molecules during development of synapses and after induction of synaptic plasticity.

Research Products

• [Journal Article] Cbln1 down-regulates the formation and function of inhibitory synapses in mouse cerebellar Purkinje cells (2014)
  • Author(s): Ito-Ishida, A., Kakegawa, W., Kohda, K., Miura, E., Okabe, S. and M. Yuzaki
  • Journal Title: European Journal of Neuroscience Volume: 39 Pages: 1268-80
  • DOI: 10.1111/ejn.12487.
  • Peer Reviewed
• [Journal Article] Dlx1 transcription factor regulates dendritic growth and postsynaptic differentiation through inhibition of neuropilin-2 and PAK3 expression (2014)
  • Author(s): Dai X., Iwasaki H., Watanabe M. and S. Okabe
  • Journal Title: European Journal of Neuroscience Volume: 39 Pages: 531-47
  • DOI: 10.1111/ejn.12413.
  • Peer Reviewed
• [Journal Article] Regulation of mitochondrial dynamics and distribution by synapse position and neuronal activity in the axon (2013)
  • Author(s): Obashi, K. and S. Okabe
  • Journal Title: European Journal of Neuroscience Volume: 38 Pages: 2350-2363
  • DOI: 10.1111/ejn.12263.
  • Peer Reviewed
• [Journal Article] Doublecortin-like kinase enhances dendritic remodeling and negatively regulates synapse maturation (2013)
  • Author(s): Shin, E., Kashiwagi, Y., Kuriu, T., Iwasaki, H., Tanaka, T., Koizumi, H., Gleeson, J. G. and S. Okabe
  • Journal Title: Nature Communications Volume: 4 Pages: 1440
  • DOI: 10.1038/ncomms2443.
  • Peer Reviewed
• [Journal Article] Presynaptically released Cbln1 induces dynamic axonal structural changes by interacting with GluD2 during cerebellar synapse formation (2012)
  • Author(s): Ito-Ishida, A., Miyazaki, T., Miura, E., Matsuda, K., Watanabe, M., Yuzaki, M and S. Okabe
  • Journal Title: Neuron Volume: 76 Pages: 549-564
  • DOI: 10.1016/j.neuron.2012.07.027.
  • Peer Reviewed
• [Journal Article] LIS1-dependent retrograde translocation of excitatory synapses in developing interneuron dendrites (2012)
  • Author(s): Kawabata, I., Kashiwagi, Y., Obashi, K., Ohkura, M., Nakai, J., Wynshaw-Boris, A., Yanagawa, Y., Okabe, S
  • Journal Title: Nature Communications Volume: 3 Pages: 722
  • DOI: 10.1038/ncomms1736.
  • Peer Reviewed
• [Journal Article] RP58 controls neuron and astrocyte differentiation by downregulating the expression of Id1-4 genes in the developing cortex (2012)
  • Author(s): Hirai, S.,Miwa, A., Ohtaka-Maruyama, C., Kasa,i M., Okabe, S., Hata, Y, and H. Okado
  • Journal Title: EMBO Journal Volume: 31 Pages: 1190-1202
  • DOI: 10.1038/emboj.2011.486.
  • Peer Reviewed
• [Journal Article] Long-term changes of spine dynamics and microglia after transient peripheral immune response triggered by LPS in vivo (2011)
  • Author(s): Kondo, S. Kohsaka, S. Okabe, S
  • Journal Title: Molecular Brain Volume: 4 Pages: 27
  • DOI: 10.1186/1756-6606-4-27.
  • Peer Reviewed
• [Journal Article] Enhancement of sciatic nerve regeneration by adenovirus-mediated expression of dominant negative RhoA and Rac1 (2011)
  • Author(s): Kusano K, Enomoto M, Hirai T, Wakabayashi Y, Itoh S, Ichinose S, Okabe S, Shinomiya K, and A. Okawa
  • Journal Title: Neuroscience Letter Volume: 492 Pages: 64-69
  • DOI: 10.1016/j.neulet.2011.01.058.
  • Peer Reviewed
• [Journal Article] Critical involvement of Rho GTPase activity in the efficient transplantation of neural stem cells into the injured spinal cord (2009)
  • Author(s): Numano, F., Inoue, A., Enomoto, M., Shinomiya, K., Okawa, A., Okabe, S
  • Journal Title: Molecular Brain Volume: 2 Pages: 37
  • DOI: 10.1186/1756-6606-2-37.
  • Peer Reviewed
• [Journal Article] Generation of cortactin floxed mice and cellular analysis of motility in fibroblasts (2009)
  • Author(s): Tanaka, S., Kunii, M., Harada, A., Okabe, S
  • Journal Title: Genesis Volume: 47 Pages: 638-646
  • DOI: 10.1002/dvg.20544.
  • Peer Reviewed
• [Journal Article] Kinase-dead knock-in mouse reveals an essential role of CaMKIIα kinase activity in dendritic spine enlargement, LTP and learning (2009)
  • Author(s): Yamagata, Y., Kobayashi, S., Umeda, T., Inoue, A., Sakagami, H., Fukaya, M., Watanabe, M., Hatanaka, N., Totsuka, M., Yagi, T., Obata, K., Imoto, K., Yanagawa, Y., Manabe, T., Okabe, S
  • Journal Title: Journal of Neuroscience Volume: 29 Pages: 7607-7618
  • DOI: 10.1523/JNEUROSCI.0707-09.2009.
  • Peer Reviewed
• [Journal Article] Enhanced synapse remodeling as a common phenotype in mouse models of autism
  • Author(s): Isshiki, M., Tanaka, S., Kuriu, T., Tabuchi, K., Takumi, T. and S. Okabe
  • Journal Title: Nature Communications Volume: (in press)
  • Peer Reviewed
• [Presentation] Imaging dynamics of postsynaptic molecules and its application in the analysis of neurodevelopmental disorders (2014)
  • Author(s): 岡 部繁男
  • Organizer: In " Imaging synapse structure and function in the vertebrate brain "
  • Place of Presentation: Janelia Farm (Ashburn, VA)
  • Year and Date: 20140330-0402
• [Presentation] Imaging synapse dynamics in vitro and in vivo (2013)
  • Author(s): 岡部繁男
  • Organizer: Plenary Lecture in International Symposium on Morphological Sciences (XXIII ISMS) 2013
  • Place of Presentation: Niigata (朱鷺メッセ)
  • Year and Date: 20130910-13
• [Presentation] Imaging analysis of synapse formation in vitro and in vivo (2013)
  • Author(s): 岡 部繁男
  • Organizer: Current Trends and Future Directions of Synaptic Plasticity Research
  • Place of Presentation: Seattle
  • Year and Date: 20130718-20
• [Presentation] Assembly and remodeling of synaptic scaffolding molecules revealed by photoactivated localization microscopy (2013)
  • Author(s): 岡部繁男
  • Organizer: In International Symposium on Super-Resolution Imaging 2013 Hamamatsu
  • Place of Presentation: オークラアクトシティホテル
  • Year and Date: 2013-12-02
• [Presentation] 中枢神経シナプス動態の可視化 (2012)
  • Author(s): 岡部繁男
  • Organizer: 第34回日本分子生物学会年会フォーラム
  • Place of Presentation: 横浜 (パシフィコ横浜)
  • Year and Date: 20121213-16
• [Presentation] Imaging analysis of synapse dynamics in vivo" Symposium "Deep tissue two-photon microscopy" (2012)
  • Author(s): 岡 部繁男
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: 京都 (京都国際会館)
  • Year and Date: 20120826-29
• [Presentation] "Imaging synapse remodeling and interactions with glia" Symposium "Development of the synapse" in ISDN 2010 (2010)
  • Author(s): 岡部繁男
  • Organizer: 18th Biennial Meeting of the International Society for Developmental Neuroscience
  • Place of Presentation: Estoril, Portugal
  • Year and Date: 20100606-09
• [Remarks]
  • URL: http://synapse.m.u-tokyo.ac.jp/