Genetic studies on unique phenotypes in MSM/Ms mouse strain

2013 Fiscal Year Final Research Report

• Project/Area Number: 21220010
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Kumamoto University
• Principal Investigator: YAMAMURA Ken-ichi 熊本大学, 生命資源研究・支援センター, 教授 (90115197)
• Co-Investigator(Kenkyū-buntansha): WAKANA Shigeharu 独立行政法人理化学研究所, バイオリソースセンター, チームリーダー (90192434) ; YAMAGATA Kazuya 熊本大学, 生命科学研究部, 教授 (70324770) ; YOSHINOBU Kumiko 熊本大学, 生命資源研究・支援センター, 助教 (20274730)
• Co-Investigator(Renkei-kenkyūsha): ARAKI Kimi 熊本大学, 生命資源研究・支援センター, 准教授 (90211705) ; TAKEDA Naoki 熊本大学, 生命資源研究・支援センター, 助教 (90304998) ; ARAKI Masatake 熊本大学, 生命資源研究・支援センター, 准教授 (80271609) ; LI Zhenghua 熊本大学, 生命資源研究・支援センター, 助教 (80398239)
• Project Period (FY): 2009-05-11 – 2014-03-31
• Keywords: 野生マウス / 膵炎 / 糖尿病 / 行動パターン / ヒト化マウス

Research Abstract

In MSM/Ms mouse, we showed that coordinate function of thalamus, midbrain, pons, and medulla was essential for its agility. In addition, high induction of Spink3 was involved in resistance to cerulein-induced pancreatitis. Furthermore, we succeeded to create knock out and knock-in mice in MODY associated genes including HNF1alpha, HNF1beta, HNF4alpha, Pdx1, NeuroD, Gck, that become valuable resources in research community.

Research Products

• [Journal Article] Behavioral and neuromorphological characterization of a novel Tuba1 mutant mouse (2012)
  • Author(s): Furuse T., Yamada I., Kushida T., Masuya H., Ikuo Miura., Kaneda H., Kobayashi K., Wada Y.,Yuasa S., Wakana S
  • Journal Title: Behavioral Brain Research Volume: 227 Pages: 167-174
  • DOI: 10.1016/j.bbr.2011.11.002
  • Peer Reviewed
• [Journal Article] Identification of hepatocyte growth factor activator (Hgfac) gene as a target of HNF1α in mouse β-cells (2012)
  • Author(s): Ohki T, Sato Y, Yoshizawa T, Yamamura K, Yamada K, Yamagata K
  • Journal Title: Biochem Biophys Res Commun Volume: 425 Pages: 619-624
  • DOI: 10.1016/j.bbrc.2012.07.134
  • Peer Reviewed
• [Journal Article] Role of intrapancreatic SPINK1/Spink3 expression in the development of pancreatitis (2012)
  • Author(s): Ohmuraya, M., Sugano, A., Hirota, M., Takaoka, Y. and Yamamura, K
  • Journal Title: Frontiers in Physiol Volume: 3 Pages: article 126
  • DOI: 10.3389/fphys.2012.00126
  • Peer Reviewed
• [Journal Article] Relationship of strain-dependent susceptibility to experimentally induced acute pancreatitis with regulation of Prss1 and Spink3 expression (2010)
  • Author(s): Wang, J., Ohmuraya, M., Suyama, K., Hirota, M., Ozaki, N., Baba, H., Nakagata, N., Araki, K. and Yamamura, K
  • Journal Title: Lab. Invest Volume: 90 Pages: 654-64
  • DOI: 10.1038/labinvest.2010.44
  • Peer Reviewed
• [Journal Article] Phenotypic characterization of anew Grin1 mutant mouse generated by ENU mutagenesis (2010)
  • Author(s): Furuse T, Wada Y., Hattori K., Yamada I., Kushida T., Shibukawa Y., Masuya H., Kaneda H., Miura I.,, Seno N., Kanda K., Hirose R.,, Toki S., Nakanishi K., Kobayashi K., Sezutsu H., Gondo Y., Noda T., Yuasa S., Wakana S
  • Journal Title: Eur. J. Neurosci Volume: 31 Pages: 1281-91
  • DOI: 10.1111/j.1460-9568.2010.07164.x
  • Peer Reviewed
• [Journal Article] Transgenic expression of pancreatic secretory trypsin inhibitor-1 rescues SPINK3 deficient mice and restores a normal pancreatic phenotype (2010)
  • Author(s): Romac, J., M. Ohmuraya, M., C. Bittner, C., Majeed, M. F., Vigna, S. R., Que, J., Fee, B. E., Wartmann, T., Yamamura, K. and Liddle, R. A
  • Journal Title: Amer. J. Physiol Volume: 298 Pages: G518-G524
  • DOI: 10.1152/ajpgi.00431.2009
  • Peer Reviewed
• [Journal Article] Establishment of germline-competent embryonic stem cell lines from the MSM/Ms strain (2009)
  • Author(s): Araki, K., Takeda, N., Yoshiki, A., Obata, Y., Nakagata, N., Shiroishi, T., Moriwaki, K. and Yamamura, K
  • Journal Title: Mamm Genome Volume: 20 Pages: 14-20
  • DOI: 10.1007/s00335-008-9160-7
  • Peer Reviewed
• [Journal Article] Effect of low-molecular-weight trypsin inhibitor, nafamostat mesilate, on trypsin activity using the pancreatic acinar cells (2009)
  • Author(s): Hashimoto, D., Ohmuraya, M., Hirota, M., Wang, J., Baba, H. and Yamamura, K
  • Journal Title: Pancreas Volume: 38 Pages: 595-597
  • DOI: 10.1097/MPA.0b013e31819156de
  • Peer Reviewed
• [Journal Article] Low serum level of high-sensitivity C-reactive protein in a Japanese subject with MODY3
  • Author(s): Ohki T, Utsu Y, Morita S, Karim M.F., Sato Y, Yoshizawa T, Yamamura K, Yamada K, Kasayama S, Yamagata K
  • Journal Title: J. Diabetes Invest Volume: (in press)
  • DOI: 10.1016/B978-0-12-800174-5.00016-8.
  • Peer Reviewed
• [Presentation] ヒト疾患モデルを用いた病因・病態解析と治療法の検証 (2014)
  • Author(s): 山村研一
  • Organizer: 第61回日本実験動物学会総会
  • Place of Presentation: 札幌(札幌コンベンションセンター)
  • Year and Date: 20140515-17
• [Presentation] オートファジー不全がもたらす慢性膵炎発症メカニズムの解析 (2013)
  • Author(s): 大村谷 昌樹,荒木喜美,山村研一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 兵庫(神戸ポートアイランド)
  • Year and Date: 20131203-06
• [Presentation] The molecular mechanisms of impaired insulin secretion in diabetes mellitus (2013)
  • Author(s): Kazuya Yamagata
  • Organizer: The 2^<nd> Shandong University-Kumamoto University Medical Symposium
  • Place of Presentation: China (Shandong Univ)
  • Year and Date: 2013-12-16
• [Presentation] Analysis of a novel HNF4a target gene Anks4b in pancreatic b-cells (2012)
  • Author(s): Kazuya Yamagata, Yoshifumi Sato, Md. Fazlul Karim, Tatsuya Yoshizawa
  • Organizer: 72^<nd> American Diabetes Association scientific sessions L
  • Place of Presentation: Hunter Limbaugh, USA (Philadelphia/Pennsylvania)
  • Year and Date: 20120608-12
• [Presentation] Spink3 is involved in pancreatitis through the regulation of autophagy (2011)
  • Author(s): Yamamura, K., Araki, K., Ohmuraya, M
  • Organizer: Mouse Genetics 2011
  • Place of Presentation: Washington DC USA (Omni Shoreham Hotel)
  • Year and Date: 20110622-25
• [Presentation] 理研BRC日本マウスクリニックII : 日本産野生系統MSMの行動解析 (2010)
  • Author(s): 山田郁子、古瀬民生、串田知子、鈴木智広、桝屋啓志、金田秀貴、小林喜美男、三浦郁生、森脇和郎、山村研一、若菜茂晴
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都(京都テルサ)
  • Year and Date: 20100512-14
• [Presentation] 膵炎におけるオートファジーの役割(シンポジウム3オートファジーと病態) (2010)
  • Author(s): 山村研一
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京(京王プラザホテル)
  • Year and Date: 20100427-29
• [Presentation] Regulation of insulin secretion by HNF-4 transcription factor (2010)
  • Author(s): Kazuya Yamagata
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会
  • Place of Presentation: 神戸ポートピアホテル(兵庫)
  • Year and Date: 2010-12-07
• [Presentation] Relationship of strain dependent susceptibility to experimentally induced acute pancreatitis with regulation of Prss1 and Spink3 expression (2009)
  • Author(s): Wang, J., Ohmuraya, M., Suyama, K., Hirota, M., Ozaki, N., Baba, H., Nakagata, N., Araki, K., Yamamura, K
  • Organizer: Fourth meeting on Asian Mouse Mutagenesis and Resource Association
  • Place of Presentation: Kumamoto
  • Year and Date: 20091217-18
• [Book] 疾患モデルマウス表現型解析指南 (2011)
  • Author(s): 山村研一、若菜茂晴, 編
  • Total Pages: 477
  • Publisher: 中山書店
• [Book] Genetic manipulations using Cre and mutant loxP sites. (ed. Alexei Morozov), in Controlled genetic manipulations (2009)
  • Author(s): Araki, K. and Yamamura, K
  • Total Pages: 29-45 (65)
  • Publisher: Neuromethods
• [Remarks]
  • URL: http://irda-genetics.kuma-u.jp/
• [Remarks]
  • URL: http://srv02.medic.kumamoto-u.ac.jp/dept/biochem2/biochem2.html
• [Remarks]
  • URL: http://www.riken.go.jp/r-world/research/lab/brc/index.html