化学物質による細胞内受容体―異物代謝酵素シグナル伝達系撹乱の感受性支配因子の解明

2009 Fiscal Year Annual Research Report

• Project/Area Number: 21221004
• Research Institution: Ehime University
• Principal Investigator: 岩田 久人 Ehime University, 沿岸環境科学研究センター, 教授 (10271652)
• Co-Investigator(Kenkyū-buntansha): 石橋 弘志 愛媛大学, 沿岸環境科学研究センター, グローバルCOE准教授 (90403857) ; 能勢 眞人 愛媛大学, プロテオ医学研究センター, 教授 (70030913)
• Keywords: シトクロムP450 (cytochrome P450) / aryl hydrocarbon receptor(AHR) / constitutive androstane receptor(CAR) / pregnane X receptor(PXR) / Peroxisome proliferator-activated receptor(PPAR) / 感受性 / 化学物質 / persistent organic pollutants(POPs)

Research Abstract

本年度得られた成果は、以下の(1)-(4)のように要約できる。
(1)細胞内受容体およびCYPのcDNAクローニング:無脊椎動物・魚類・両生類・鳥類・ほ乳類に属する生物種の細胞内受容体(またはそのホモログ)、およびCYP1-4ファミリーに属する分子種の全長cDNAのクローン化に成功した。また、これまでに報告されてこなかった新規の細胞内受容体・CYP分子種も新たに発見した。(2)CYP遺伝子プロモーター/エンハンサー領域のクローニング:魚類・両生類・鳥類・ほ乳類に属する生物種のCYP遺伝子プロモーター/エンハンサー領域のクローニングに成功した。in silico系およびin vitro系での解析により、細胞内受容体特異的な反応部位を同定することができた。これらの結果により、細胞内受容体-CYPシグナル伝達系の進化および生物種特異性の一端が明らかになった。(3)CYPによる化学物質代謝能の解明と代謝経路・産物の網羅的解析:鳥類・ほ乳類のCYP1A分子種の全長cDNAのクローンを用いて、これら遺伝子のin vitro系による蛋白質の発現に成功した。またエトキシレゾルフィンやPCBなどの化学物質(基質)とこれら蛋白質を反応させることにより、代謝産物を同定することができた。得られた代謝産物の種類・量から、進化学的近縁種であってもCYP1の代謝能は種間で大きく異なることがわかった。(4)細胞内受容体およびCYP以外の感受性規定因子の探索:組換え近交系マウス各系統の脾細胞を2,3,7,8-四塩素化ダイオキシンで処理し、CYP1A1遺伝子発現量の用量依存性を解析したところ、マウス各系統群間でのEC_<50>等の用量一応答パラメーターに明確な差が認められた。これら感受性の差はマウス各系統のAHRアミノ酸配列のみでは説明できなかった。したがって、この差はAHR以外の要因が関与していると推察された。

Research Products

• [Journal Article] Dioxin-like and perfluorinated compounds in pigs in an Indian open waste dumping site : toxicokinetics and effects on hepatic cytochrome P450 and blood plasma hormones (2010)
  • Author(s): Watanabe, M.X., Kunisue, T., Tao, L., Kannan, K., Subramanian, An., Tanabe, S., Iwata, H.
  • Journal Title: Environmental Toxicology and Chemistry (in press)
  • Peer Reviewed
• [Journal Article] Decolorization and estrogenic activity of colored livestock wastewater by electrolysis system (2010)
  • Author(s): Kai, H., Ishibashi, Y., Mori, T., Ishibashi, H., Kawaguchi, T., Ohwaki, H., Arizono, K., Takemasa, T.
  • Journal Title: Material Recycle and Waste Management Research (in press)
  • Peer Reviewed
• [Journal Article] Relationship of urinary arsenic metabolites to intake estimates in residents of the Red river delta, Vietnam (2009)
  • Author(s): Agusa, T., Kunito, T., Minh, T.B., Kim Trang, P.T., Iwata, H., Viet, P.H., Tanabe, S.
  • Journal Title: Environmental Pollution 157 Pages: 396-403
  • Peer Reviewed
• [Journal Article] Accumulation features and temporal trends of PCDDs, PCDFs and PCBs in Baikal seals(Pusa sibirica) (2009)
  • Author(s): Imaeda, D., Kunisue, T., Ochi, Y., Iwata, H., Tsydenova, O., Takahashi, S., Amano, M., Petrov, E.A., Batoev, V.B., Tanabe, S.
  • Journal Title: Environmental Pollution 157 Pages: 737-747
  • Peer Reviewed
• [Journal Article] Effects of nonylphenol on growth and 20-hydroxyecdysone levels in mysid crustacean, Americamysis bahia (2009)
  • Author(s): Hirano, M., Ishibashi, H., Kim, J.W., Matsumura, N., Arizono, K.
  • Journal Title: Comparative Biochemistry and Physiology Part C : Toxicology and Pharmacology 149 Pages: 369-373
  • Peer Reviewed
• [Journal Article] Alkoxyresorufin(methoxy-, ethoxy-, pentoxy- and benzyloxyresorufin)O-dealkylase activities by in vitro-expressed cytochrome P450 1A4 and 1A5 from common cormorant(Phalacrocorax carbo) (2009)
  • Author(s): Kubota, A., Kim, E.Y., Iwata, H.
  • Journal Title: Comparative Biochemistry and Physiology, Part C Toxicology and Pharmacology 149 Pages: 544-551
  • Peer Reviewed
• [Journal Article] Effects of co-exposure to 2,3,7,8-tetrachlorodihenzo-p-dioxin and perfluorooctane sulfonate or perfluorooctanoic acid on expression of cytochrome P450 isoforms in chicken(Gallus gallus)embryo hepatocyte cultures (2009)
  • Author(s): Watanabe, M.X., Jones, S.P., Iwata, H., Kim, E.Y., Kennedy, S.W.
  • Journal Title: Comparative Biochemistry and Physiology, Part C Toxicology and Pharmacology 149 Pages: 605-612
  • Peer Reviewed
• [Journal Article] 8-hydroxy-2'-deoxyguanosine(8-OHdG)as a possible marker of arsenic poisoning : A clinical case study on the relationship between concentrations of 8-OHdG and each arsenic compound in urine of an acute promyelocytic leukemia patient being treated with arsenic trioxide (2009)
  • Author(s): Fujihara, J., Agusa, T., Tanaka, J., Fujii, Y., Moritani, T., Hasegawa, M., Iwata, H., Tanabe, S., Takeshita, H.
  • Journal Title: Forensic Toxicology 27 Pages: 41-44
  • Peer Reviewed
• [Journal Article] Transactivation potencies of Baikal seal constitutive active/androstane receptor by persistent organic pollutants and brominated flame retardants (2009)
  • Author(s): Sakai, H., Kim, E.Y., Petrov, E.A., Tanabe, S., Iwata, H.
  • Journal Title: Environmental Science and Technology 43 Pages: 6391-6397
  • Peer Reviewed
• [Journal Article] Dioxin activation of CYP1A5 promoter/enhancer regions from two avian species, common cormorant(Phalacrocorax carbo)and chicken(Gallus gallus): Association with aryl hydrocarbon receptor 1 and 2 isoforms (2009)
  • Author(s): Lee, J.S., Kim, E.Y., Iwata, H.
  • Journal Title: Toxicology and Applied Pharmacology 234 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Genetic polymorphisms in AS3MT and arsenic metabolism in residents of the red river delta, Vietnam (2009)
  • Author(s): Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T.B., Trang, P.T., Viet, P.H., Tanabe, S.
  • Journal Title: Toxicology and Applied Pharmacology 236 Pages: 131-141
  • Peer Reviewed
• [Journal Article] Genetic polymorphisms in glutathione S-transferase(GST)superfamily and arsenic metabolism in residents of the Red River Delta, Vietnam (2009)
  • Author(s): Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T.B., Trnag, P.T., Viet, P.H., Tanabe, S.
  • Journal Title: Toxicology and Applied Pharmacology 242 Pages: 352-362
  • Peer Reviewed
• [Journal Article] Human exposure to arsenic from groundwater in the red river and Mekong river deltas in Vietnam (2009)
  • Author(s): Agusa, T., Inoue, S., Kunito, T., Minh, T.B., Ha, N.N., Tu, N.P.C., Kim Trang, P.T., Iwata, H., Viet, P.H., Tuyen, B.C., Tanabe, S.
  • Journal Title: International Journal of Environmental Studies 66 Pages: 49-57
  • Peer Reviewed
• [Journal Article] Acute toxicity of pharmaceutical and personal care products on freshwater crustacean(Thamnocephalus platyurus)and fish(Oryzias latipes) (2009)
  • Author(s): Kim, J.W., Ishibashi, H., Yamauchi, R., Ichikawa, N., Takao, Y., Hirano, M., Koga, M., Arizono, K.
  • Journal Title: The Journal of Toxicological Sciences 34 Pages: 227-232
  • Peer Reviewed
• [Journal Article] Occurrence of pharmaceutical and personal care products(PPCPs)in surface water from Mankyung River, South Korea (2009)
  • Author(s): Kim, J.W., Jang, H.S., Kim, J.G., Ishibashi, H., Hirano, M., Nasu, K., Ichikawa, N., Takao, Y., Shinohara, R., Arizono, K.
  • Journal Title: Journal of Health Science 55 Pages: 249-258
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• [Journal Article] Expression analysis of estrogen-responsive genes vitellogenin 1 and 2 in liver of male medaka(Oryzias latipes)exposed to selective ligands of estrogen receptor subtypes (2009)
  • Author(s): Yamaguchi, A., Ishibashi, H., Kohra, S., Arizono, K., Kato, K., Nakahama, T., Kanno, Y., Inouye, Y., Tominaga, N.
  • Journal Title: Journal of Health Science 55 Pages: 930-938
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• [Journal Article] Aneurysms of the renal arteries associated with segmental arterial mediolysis in a case of polyarteritis nodosa (2009)
  • Author(s): Soga, Y., Nose, M., Arita, N., Komor, H., Miyazaki, T., Maeda, T., Furuya, K.
  • Journal Title: Pathology Int. 59 Pages: 197-200
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• [Journal Article] Charactrization of lymphangiogenesis in various stages of idiopathic diffuse alveolar damage (2009)
  • Author(s): Yamashita, M., Iwama, N., Date, F., Chiba, R., Ebina, M., Miki, H., Yamauchi, K., Sawai, T., Nose, M., Sato, S., Takahashi, T., Ono, M.
  • Journal Title: Human pathology. 40 Pages: 542-551
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• [Journal Article] Toll-Like receptor 3 signaling induces chronic pancreatitis through the Fas/Fas ligand-mediated cytotoxicity (2009)
  • Author(s): Soga, Y., Komori, H., Miyazaki, T., Arita, N., Terada, M., Kamada, K., Tanaka, Y., Fujino, T., Hiasa, Y., Matsuura, B., Onji, M., Nose, M.
  • Journal Title: Tohoku J.Exp.Med. 217 Pages: 175-184
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• [Journal Article] Expression of sphingosine kinase 2 in synovial fibroblasts of rheumatoid arthritis contributing to apoptosis by a shingosine analogue, FTY720 (2009)
  • Author(s): Kamada, K., Arita, N., Tsubaki, T., Takubo, N., Fujino, T., Soga, Y., Miyazaki, T., Yamamoto, H., Nose, M.
  • Journal Title: Pathology Int. 59 Pages: 382-389
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• [Journal Article] Genetic dissociation of dacryoadenitis and sialadenitis in a sjogren's syndrome mouse model with common and different susceptibility gene loci (2009)
  • Author(s): Kamao, T., Miyazaki, T., Soga, Y., Komori, H., Terada, M., Ohashi, Y., Nose, M.
  • Journal Title: Invest Ophthalmol Vis Sci. 50 Pages: 3257-3265
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• [Journal Article] Hexabromocyclododecanes in human adipose tissue from Japan (2009)
  • Author(s): Isobe, T., Oda, H., Takayanagi, N., Kunisue, T., Komori, H., Arita, N., Ueda, N., Nos, M., Yamada, T., Takahashi, S., Tanabe, S.
  • Journal Title: Environ.Chem. 6 Pages: 328-333
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• [Journal Article] Evaluating the role of rheumatoid factors in the development of rheumatoidarthritis in a mouse model with a newly established ELISA system (2009)
  • Author(s): Tanaka, Y., Komori, H., Mori, S., Soga, Y., Tsubaki, T., Terada, M., Miyazaki, T., Fujino, T., Nakamura, S., Kanno, H., Sawasaki, T., Endo, Y., Nose, M.
  • Journal Title: Tohoku J Exp Med. 220 Pages: 199-206
  • Peer Reviewed
• [Journal Article] Role of SLAM-associated protein in the pathogenesis of autoimmune diseases and immunological disorders (2009)
  • Author(s): Furukawa, H., Tohma, S., Kitazawa, H., Komori, H., Nose, M., Ono, M.
  • Journal Title: Arch Immunol Ther Exp. 58 Pages: 37-44
  • Peer Reviewed
• [Journal Article] 関節リウマチの滑膜増殖制御の新展開-マイクロアレーによる制御遺伝子の探索- (2009)
  • Author(s): 鎌田一億・有田典正,・椿崇仁・宮崎龍彦・藤野貴広・能勢眞人
  • Journal Title: 臨床と研究 86 Pages: 686-692
• [Journal Article] モデルマウスから学ぶ血管炎のゲノム的仕組み (2009)
  • Author(s): 能勢眞人,・小森浩章
  • Journal Title: J Jpn Coll Angiol. 49 Pages: 11-16
• [Journal Article] 血管炎発症のポリジーンネットワークモデル (2009)
  • Author(s): 能勢眞人・小森浩章
  • Journal Title: リウマチ科 42 Pages: 337-343
• [Presentation] Arsenic, manganese and barium contamination in groundwater and human in Vietnam (2010)
  • Author(s): Agusa, T., Inoue, S., Kunito, T., Minh, T.B., Tu, N.P.C., Ha, N.N., Kim Trang, P.T., Iwata, H., Tuyen, B.C., Viet, P.H., Tanabe, S.
  • Organizer: The first international conference on environmental pollution, restoration and management [SETAC A/P Joint Conference]
  • Place of Presentation: Ho Chi Mink City, Vietnam
  • Year and Date: 20100301-20100305
• [Presentation] 半網羅的ディファレンシャル・ディスプレイ法システムの構築 (2009)
  • Author(s): 寺岡宏樹・池田晴喜・伊藤知子・鵜飼典佳・白石順也・金恩英・岩田久人・遠藤大二・平賀武夫
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜、日本
  • Year and Date: 20091209-20091212
• [Presentation] CYP1A transactivation potencies of the black-footed albatross AhR1 and and AhR2 by dioxins and risk assessment in the North Pacific population (2009)
  • Author(s): Thuruthippallil, L.M., Kim, E.Y., Iwata, H.
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] Functional characterization of cytochrome P450 1A1, 1A2 and 1B1 from the Baikal seal(Pusa sibirica)heterologously expressed in yeast cells (2009)
  • Author(s): Yamaguchi, K., Kim, E.Y., Kubota, A., Iwata, H.
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] Cellular localization of the AHR isoforms(AHR1 and AHR2)from avian species (2009)
  • Author(s): Nomaru, K., Kim, E.Y., Inoue, N., Yasui, T., Iwata, H.
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] ニワトリ新規AHR1アイソフォーム(2^<nd> AHR1)の機能解析 (2009)
  • Author(s): 岩渕康平・金恩英・李鎮善・安井知子・岩田久人
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] Effects of TCDD on the AHR-CYP1A signaling cascade in avian developing embryos (2009)
  • Author(s): Iwata, H., Nagahama, N., Watanabe, X.M., Kim, E.Y.
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] Molecular characterization of peroxisome proliferators-activated receptor β/σ from the Baikal seal(Pusa sibirica) (2009)
  • Author(s): Ishibashi, H., Kim, E.Y., Tanabe, S., Iwata, H.
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] Development of an in vitro reporter gene assay for screening the ecdysone receptor agonists in mysid crustacean, Americamysis bahia (2009)
  • Author(s): Hirano, M., Ishibashi, H., Kim, E.Y., Arizono, K., Iwata, H.
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] Morphological changes in peripheral nervous system by TCDD in developing red seabream(Pagrus major)embryos (2009)
  • Author(s): Iida, M., Kim, E.Y., Murakami, Y., Shima, Y., Iwata, H.
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] Identification and expression profile of CYP1-4 genes in Xenopus laevis (2009)
  • Author(s): Suzuki, K., Iwata, H.
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] 環境化学物質に曝露したメダカにおけるDNAマイクロアレイを用いた遺伝子発現解析 -第1報- (2009)
  • Author(s): 竹元望・石橋弘志・平野将司・金俊佑・山内良子・孫〓富・内田雅也・有菌幸司
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] メダカ繁殖試験によるフッ素テロマーアルコールの影響評価 (2009)
  • Author(s): 山内良子・石橋弘志・内田雅也・孫〓富・竹元望・竹本直道・金俊佑・平野将司・有薗幸司
  • Organizer: 第12回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091207-20091208
• [Presentation] Risk Assessment of dioxins in the North Pacific black-footed albatross based on CYP1A transactivation potencies of the aryl hydrocarbon receptor 1 and 2(AhR1 and AhR2) (2009)
  • Author(s): Thuruthippallil L.M., Kim, E.Y., Iwata, H.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] Association of inorganic arsenic metabolism with genetic polymorphisms of GST isoforms in North Vietnamese populations : Utilization of stored samples in the environmental specimen bank of Ehime University (2009)
  • Author(s): Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T.B., Kim Trang, P.T., Viet, P.H., Tanabe, S.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] Functional characterization of in vitro-expressed cytochrome P450 1A1, 1A2, and 1B1 from Baikal seal(Pusa sibirica) (2009)
  • Author(s): Yamaguchi, K., Kim, E.Y., Iwata H.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] Identification and characterization of cytochrome P450 and xenobiotics receptor genes in amphibians, Xenopus laevis and Xenopus tropicalis (2009)
  • Author(s): Suzuki, K., Iwata, H.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] A preliminary study on chemical ligand screening of the constitutive androstane receptor(CAR)using a surface plasmon resonance array system (2009)
  • Author(s): Dau, P.T., Ishibashi, H., Kim, E.Y., Iwata H.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] Quantitative assessment of the AHR-CYP1A signaling cascase in TCDD-treated avian embryos (2009)
  • Author(s): Iwata, H., Nagahama, N., Watanabe, M., Kim, E.Y.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on peripheral nervous system in developing red seabream(Pugrus major)embryos (2009)
  • Author(s): Iida, M., Kim, E.Y., Murakami, Y., Shima, Y., Iwata, H.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] Moleculaz cloning of the ecdysone receptor from a mysid crustacean(Americamysis bahia)and development of an in vitro reporter gene assay to detect the receptor-mediated responses by chemicals (2009)
  • Author(s): Hirano, M., Ishibashi, H., Kim, E.Y., Arizono, K., Iwata, H.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] Effects of dioxin-like and perfluorinated compounds on hepatic cytochrome P450 and plasma hormones in pigs from an indian open waste dumping site (2009)
  • Author(s): Watanabe, X.M., Kunisue, T., Tao, L., Kannan, K., Subramanian, A., Tanabe, S., Iwata, H.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] Intracellular localization of the AHR2 isoform from avian species (2009)
  • Author(s): Nomaru, K., Kim, E.Y., Inoue, N., Yasui, T., Iwata, H.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] Molecular characterization of peroxisome proliferator-activated receptor isoforms from the Baikal seal(Pusa sibirica) (2009)
  • Author(s): Ishibashi, H., Kim, E.Y., Tanabe, S., Iwata, H.
  • Organizer: International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20091203-20091205
• [Presentation] ベトナムのヒ素汚染地域住民におけるAS3MT遺伝子多型とヒ素代謝の関係 (2009)
  • Author(s): 阿草哲郎・岩田久人・藤原純子・國頭恭・Minh, T.B.・Kim Trang, P.T.・Viet, P.H.・竹下治男・田辺信介
  • Organizer: 第15回ヒ素シンポジウム
  • Place of Presentation: 大阪、日本
  • Year and Date: 20091128-20091129
• [Presentation] 環境汚染物質による野生生物のリスクを評価するための科学的方法 (2009)
  • Author(s): 岩田久人・金恩英
  • Organizer: 日本環境変異原学会第38回大会
  • Place of Presentation: 静岡、日本
  • Year and Date: 20091126-20091127
• [Presentation] ベトナムにおけるAS3MT遺伝子多型とヒ素代謝能の関係 (2009)
  • Author(s): 阿草哲郎・岩田久人・藤原純子・國頭恭・Minh, T.B.・Kim Trang, P.T.・Viet, P.H.・竹下治男・田辺信介
  • Organizer: 日本DNA多型学会第18回学術集会
  • Place of Presentation: 久留米市、日本
  • Year and Date: 20091118-20091120
• [Presentation] 膠原病のポリジーンネットワーク(シンポジウム) (2009)
  • Author(s): 能勢眞人
  • Organizer: 第37回日本臨床免疫学会総会
  • Place of Presentation: 東京(東京ステーションコンファレンス)
  • Year and Date: 20091113-20091115
• [Presentation] 線虫 C. elegans を用いた食品中機能性成分の抗酸化作用評価 (2009)
  • Author(s): 山元涼子・井口綾子・内匠正太・上田清佳・石橋弘志・冨永伸明・有薗幸司
  • Organizer: フォーラム2009衛生薬学・環境トキシコロジー
  • Place of Presentation: 沖縄、日本
  • Year and Date: 20091105-20091106
• [Presentation] ERα/ERβ選択性リガンドを用いたメダカvtg1/2の発現誘導解析 (2009)
  • Author(s): 山口明美・石橋弘志・高良真也・有園幸司・加藤恵介・中浜隆之・菅野雄一朗・井上義雄・冨永伸明
  • Organizer: フォーラム2009衛生薬学・環境トキシコロジー
  • Place of Presentation: 沖縄、日本
  • Year and Date: 20091105-20091106
• [Presentation] 血管炎に関する基礎研究の進歩(シンポジウム) (2009)
  • Author(s): 能勢眞人
  • Organizer: 第50回日本脈管学会総会
  • Place of Presentation: 東京(ハイアットリージェンシー東京)
  • Year and Date: 20091029-20091031
• [Presentation] Functional characterization of AHR isoforms, AHR1, AHR2 and AHRR in avian species (2009)
  • Author(s): Kim, E.Y., Lee, J.S., Inoue, N., Yasui, T., Iwata, H.
  • Organizer: 21^<st> Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20091026-20091027
• [Presentation] Quantitative analyses of AHR-CYP1A signaling in avian developing embryos (2009)
  • Author(s): Iwata, H., Nagahama, N., Watanabe, M., Kim, E.Y.
  • Organizer: 21^<st> Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20091026-20091027
• [Presentation] Characterization of ecdysone receptor and ultraspiracle mRNA through molting period in mysid crustacean (2009)
  • Author(s): Hirano, M., Ishibashi, H., Yamauchi, R., Kim, J.W., Arizono, K.
  • Organizer: 21^<st> Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20091026-20091027
• [Presentation] Transcriptional modulation of estrogen-responsive genes in male medaka(Oryzias latipes)after exposure to synthetic polycyclic musks (2009)
  • Author(s): Ishibashi, H., Yamauchi, R., Kim, J.W., Hirano, M., Mori, T., Tominaga, N., Arizono, K.
  • Organizer: 21^<st> Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20091026-20091027
• [Presentation] Characterization of aryl hydrocarbon receptor in avian species (2009)
  • Author(s): Fujisawa, N., Tanaka, K., Yamamoto, H., Ikenaka, Y., Kim, E.Y., Lee, J.S., Iwata, H., Ishizuka, M.
  • Organizer: 21^<st> Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20091026-20091027
• [Presentation] メダカDNAマイクロアレイを用いたエストロゲン様物質により変動する遺伝子群の網羅的解析 (2009)
  • Author(s): 内田雅也・石橋弘志・平野将司・金俊佑・冨永伸明・山内良子・有薗幸司
  • Organizer: 第15回日本環境毒性学会・バイオアッセイ研究会合同研究発表会
  • Place of Presentation: 東京、日本
  • Year and Date: 20091003-20091004
• [Presentation] Disruption of aryl hydrocarbon receptor-cytochrome P450 1A signaling pathway by dioxin-like chemicals in wildlife (2009)
  • Author(s): Iwata, H, Kim, E.Y.
  • Organizer: BIOLOGICAL RESPONSES TO CHEM-CAL CONTAMINANTS : from molecular to community level, Interdisciplinary Symposium for Young Investigators
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 20090902-20090904
• [Presentation] Interindividual variation in arsenic metabolism in a Vietnamese population : association with single nucleotide polymorphisms in AS3MT (2009)
  • Author(s): Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T.B., Kim, P.T., Viet, P.H., Tanabe, S.
  • Organizer: BIOLOGICAL RESPONSES TO CHEMICAL CONTAMINANTS : from molecular to community level Interdisciplinary Symposium for Young Investigators
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 20090902-20090904
• [Presentation] 有機フッ素化合物によるヒメダカ肝臓中CYP3As発現誘導 (2009)
  • Author(s): 山内良子・石橋弘志・平野将司・金俊佑・高尾雄二・有薗幸司.
  • Organizer: 第36回日本トキシコロジー学会学術年会
  • Place of Presentation: 岩手、日本
  • Year and Date: 20090706-20090708
• [Presentation] 線虫DNAマイクロアレイによるリチウムの影響評価 (2009)
  • Author(s): 井口綾子・内匠正太・山元涼子・石橋弘志・冨永伸明・有薗幸司.
  • Organizer: 第36回日本トキシコロジー学会学術年会
  • Place of Presentation: 岩手、日本
  • Year and Date: 20090706-20090708
• [Presentation] ベトナム人におけるGSTスーパーファミリーの遺伝子多型と尿中ヒ素化合物プロファイルの関係 (2009)
  • Author(s): 阿草哲郎・岩田久人・藤原純子・國頭恭・竹下治男・Minh, T.B.・Kim Trang, P.T.・Viet, P.H.
  • Organizer: 第20回日本微量元素学会学術集会
  • Place of Presentation: 東京、日本
  • Year and Date: 20090702-20090703
• [Presentation] ベトナム人のヒ素曝露とその代謝遺伝子多型 (2009)
  • Author(s): 阿草哲郎・岩田久人・藤原純子・國頭恭・Minh, T.B.・Kim Trang, P.T.・Vie, P.H.・竹下治男・田辺信介
  • Organizer: 第18回環境化学討論会
  • Place of Presentation: つくば市、日本
  • Year and Date: 20090609-20090611
• [Presentation] Functional analysis of the aryl hydrocarbon receptor repressor from chicken (2009)
  • Author(s): Lee, J.S., Kim, E.Y., Iwata, H.
  • Organizer: 15th International Symposium on Pollutant Responses in Marine Organisms
  • Place of Presentation: Bordeaux, France
  • Year and Date: 20090317-20090320
• [Presentation] CYP1A transactivation potencies of aryl hydrocarbon receptor 1 and 2(AHR1 and AHR2)from black-footed albatross by dioxin-like congeners (2009)
  • Author(s): Thuruthippallil, L.M., Kim, E.Y., Iwata, H.
  • Organizer: 15th International Symposium on Pollutant Responses in Marine Organisms
  • Place of Presentation: Bordeaux, France
  • Year and Date: 20090317-20090320
• [Presentation] Catalytic function of in vitro-expressed Baikal seal CYP1A1 (2009)
  • Author(s): Yamaguchi, K., Kim, E.Y., Iwata, H.
  • Organizer: 15th International Symposium on Pollutant Responses in Marine Organisms
  • Place of Presentation: Bordeaux, France
  • Year and Date: 20090317-20090320
• [Presentation] Characterization of aryl hydrocarbon receptor-cytochrome P450 1A signaling pathway in wildlife (2009)
  • Author(s): Iwata, H, Kim, E.Y.
  • Organizer: International Symposium on the Biomonitoring and Biomarker of Endocrine Disrupting chemicals(EDCs)in Coastal and Marine Environment
  • Place of Presentation: Busan, Korea
  • Year and Date: 2009-09-17
• [Book] Risk assessment of dioxins in wild birds by the combination of contamination level and species-specific response of aryl hydrocarbon receptor, Proceeding Book "Interdisciplinary Studies on Environmental Chemistry, Vol. 3", (2010)
  • Author(s): 岩田久人, 金恩英
  • Publisher: Terra Scientific Publishing Company(in press)
• [Book] 分子でよむ環境汚染(鈴木聡編)第5章 生物反応を利用して化学物質汚染の危険性を検知する (2009)
  • Author(s): 岩田久人・金恩英
  • Total Pages: 35
  • Publisher: 東海大学出版会
• [Remarks]
  • URL: http://ecotoxiwata.jp/sr-s.html