化学物質による細胞内受容体―異物代謝酵素シグナル伝達系撹乱の感受性支配因子の解明

2010 Fiscal Year Annual Research Report

• Project/Area Number: 21221004
• Research Institution: Ehime University
• Principal Investigator: 岩田 久人 愛媛大学, 沿岸環境科学研究センター, 教授 (10271652)
• Co-Investigator(Kenkyū-buntansha): 能勢 眞人 愛媛大学, プロテオ医学研究センター, 教授 (70030913) ; 石橋 弘志 愛媛大学, 沿岸環境科学研究センター, グローバルCOE准教授 (90403857) ; 鈴木 賢一 愛媛大学, 沿岸環境科学研究センター, グローバルCOE助教 (90363043)
• Keywords: シトクロムP450 (cytochrome P450) / aryl hydrocarbon receptor (AHR) / constitutive androstane receptor (CAR) / pregnane X receptor (PXR) / peroxisome proliferator-activated receptor (PPAR) / 感受性 / 化学物質 / persistent organic pollutants (POPs)

Research Abstract

本年度得られた成果は、以下の(1)-(5)のように要約できる。
(1)CYP遺伝子プロモーター/エンハンサー領域の解析:魚類および両生類のCYP1ファミリーに属する遺伝子のプロモーター/エンハンサー領域の塩基配列を解析し、アリルハイドロカーボン受容体(AHR)が結合する特定配列が各遺伝子上流域に存在することがわかった。この配列の位置・数には種・遺伝子特異性が認められた。
(2)CYP遺伝子のクローニング:カワウの肝臓cDNAライブラリーから、4種類のCYP2分子種(CYP2AC1・CYP2AF1・CYP2C45・CYP2J25)を同定した。さらにカワウ肝臓の各CYP2 mRNA発現量と化学物質蓄積濃度との関係を解析し、CYP2C45・CYP2J25の発現は有機フッ素化合物暴露により抑制されることを示唆した。
(3)細胞内受容体と相互作用する化学物質の網羅的解析:水棲哺乳類のAHRおよびペルオキシソーム増殖剤応答性受容体(PPAR)を発現させたレポーター遺伝子アッセイ系を構築し、ダイオキシン類や有機フッ素系化合物による標的遺伝子転写活性化能について測定した。さらにこれら化学物質の立体構造と各物質の相対効力の関係を解析し、構造-活性相関を見出した。
(4)CYPによる化学物質代謝能の解明と代謝経路・産物の網羅的解析:水棲哺乳類CYP1分子種を対象に、酵母によるCYPタンパク質発現系を構築した。本発現系を利用して、アルコキシレゾルフィンやエストロジェンを基質とした各CYP1分子種の代謝パラメーターを代謝産物毎に測定した。
(5)細胞内受容体およびCYP以外の感受性規定因子の探索:マウス各系統の脾細胞をTCDD処理し、CYP1A1遺伝子発現量の用量依存性の系統差を調査した。その結果、AHRの遺伝子型は鈍感型であるにもかかわらず、CYP1A1発現誘導能が高いマウス系統が存在することを確認した。また、これらマウス系統のCYP1A1遺伝子の上流域には差が認められなかったことから、感受性差はAHRやCYP以外の要因が関与していると推察された。

Research Products

• [Journal Article] Evaluation of relative potencies for in vitro transactivation of the Baikal seal aryl hydrocarbon receptor by dioxin-like compounds. (2011)
  • Author(s): Kim, E.Y., Suda, T., Tanabe, S., Batoev, V.B., Petrov, E.A., Iwata, H.
  • Journal Title: Environmental Science & Technology Volume: 45 Pages: 1652-1658
  • Peer Reviewed
• [Journal Article] Molecular and functional characterization of aryl hydrocarbon receptor repressor from the chicken (Gallus gallus) : interspecies similarities and differences. (2011)
  • Author(s): Lee, J.S., Kim, E.Y., Nomaru, K., Iwata, H.
  • Journal Title: Toxicological Sciences Volume: 119 Pages: 319-334
  • Peer Reviewed
• [Journal Article] Intake and excretion of arsenicals in green (Chelonia mydas) and hawksbill turtles(Eretmochelys imbricata). (2011)
  • Author(s): Agusa, T., Takagi, K., Miller, T.W., Kubota, R., Anan, Y., Iwata, H., Tanabe, S.
  • Journal Title: Environmental Chemistry Volume: 8 Pages: 19-29
  • Peer Reviewed
• [Journal Article] 8-Hydroxy-2'-deoxyguanosine and arsenic compounds in urine and serum of a 4-year-old child suffering from acute promyelocytic leukemia during treatment with arsenic trioxide. (2011)
  • Author(s): Fujihara, J., Hasegawa, M., Kanai, R., Agusa, T., Iwata, H., Tanabe, S., Yasuda, T., Yamaguchi, S., Takeshita, H.
  • Journal Title: Forensic Toxicology Volume: 29 Pages: 65-68
  • Peer Reviewed
• [Journal Article] Dioxin-like and perfluorinated compounds in pigs in an Indian open waste dumping site : toxicokinetics and effects on hepatic cytochrome P450 and blood plasma hormones. (2010)
  • Author(s): Watanabe, M.X., Kunisue, T., Tao, L., Kannan, K., Subramanian, An., Tanabe, S., Iwata, H.
  • Journal Title: Environmental Toxicology and Chemistry Volume: 29 Pages: 1551-1560
  • Peer Reviewed
• [Journal Article] Hepatic CYP1A introduction by chlorinated dioxins and related compounds in the endangered black-footed albatross from the North Pacific. (2010)
  • Author(s): Kubota, A., Watanabe, M., Kunisue, T., Kim, E.Y., Tanabe, S., Iwata, H.
  • Journal Title: Environmental Science & Technology Volume: 44 Pages: 3559-3565
  • Peer Reviewed
• [Journal Article] Effects of in ovo exposure to 2,3,7,8-tetrachlorodibenzo-p-doxin on hepatic AHR/ARNT CYP1A signaling pathways in common cormorants (Phalacrocoraxcarbo). (2010)
  • Author(s): Iwata, H., Nagahama, N., Kim, E.Y., Watanabe, M.X., Sudo, A.
  • Journal Title: Comparative Biochemistry and Physiology, Part C Volume: 152 Pages: 224-231
  • Peer Reviewed
• [Journal Article] Exposure, metabolism, and health effects of arsenic in residents from arsenic-contaminated groundwater areas of Vietnam and Cambodia : A review. (2010)
  • Author(s): Agusa, T., Kunoto, T., Kubota, R., Inoue, S., Fujihara, J., Minh, T.B., Ha, N.N., Tu, N.P.C., Trang, P.T.K., Chamnan, C., Takeshita, H., Iwata, H., Tuyen, B.C., Viet, P.H., Tana, T.S., Tanabe, S.
  • Journal Title: Reviews on Environmental Health Volume: 25 Pages: 193-220
  • Peer Reviewed
• [Journal Article] A microarray data analysis method to evaluate the impact of contaminants on wild animals. (2010)
  • Author(s): Nakayama, K., Handoh, I.C., Kitamura, S., Kim, E.Y., Iwata, H.,Tanabe, S.
  • Journal Title: Science of the Total Environment Volume: 408 Pages: 5824-5827
  • Peer Reviewed
• [Journal Article] Evaluating the role of rheumatoid factors in the development of rheumatoidarthritis in a mouse model with a newly established ELISA system. (2010)
  • Author(s): Tanaka, Y., Komori, H., Mori, S., Soga, Y., Tsubaki, T., Terada, M., Miyazaki, T., Fujino, T., Nakamura, S., Kanno, H., Sawasaki, T., Endo, Y., Nose, M.
  • Journal Title: The Tohoku Journal of Experimental Medicine. Volume: 220 Pages: 199-206
  • Peer Reviewed
• [Journal Article] Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis. (2010)
  • Author(s): Matsuoka, K., Komori, H., Nose, M., Endo, Y., Sawasaki, T.
  • Journal Title: Journal of Proteome Research Volume: 9 Pages: 4264-4273
  • Peer Reviewed
• [Journal Article] Role of SLAM-associated protein in the pathogenesis of autoimmune diseases and immunological disorders. (2010)
  • Author(s): Furukawa, H., Tohma, S., Kitazawa, H., Komori, H., Nose, M., Ono, M.
  • Journal Title: Archivum Immunologiae et Therapia Experimentalis Volume: 58 Pages: 37-44
  • Peer Reviewed
• [Journal Article] Genetic heterogeneity in rheumatoid arthritis mouse models induced by extrinsic and intrinsic factors. (2010)
  • Author(s): Mizuki, S., Oishi, H., Zhang, M.C., Kamogawa, J., Miyazaki, T., Ono, M., Takahashi, S., Yamamoto, H., Nose, M.
  • Journal Title: Pathology International Volume: 60 Pages: 430-437
  • Peer Reviewed
• [Journal Article] Contamination of pharmaceutical and personal care products in sewage treatment plants and surface waters in South Korea and their removal during activated sludge treatment. (2010)
  • Author(s): Kim, J.W., Ishibashi, H., Hirano, M., Jang, H.S., Kim, J.G., Takao, Y., Ichikawa, N., Shinohara, R., Arizono, K.
  • Journal Title: Japan Journal of Environmental Chemistry Volume: 20 Pages: 127-136
  • Peer Reviewed
• [Journal Article] Decolorization and estrogenic activity of colored livestock watewater by electrolysis system. (2010)
  • Author(s): Kai, H., Ishibashi, Y., Mori, T., Ishibashi, H., Kawaguchi, T., Ohwaki, H., Arizono, K., Takemasa, T.
  • Journal Title: Material Recycle and Waste Management Research Volume: 12 Pages: 128-135
  • Peer Reviewed
• [Journal Article] Characterization of a novel type I keratin gene and generation of transgenic lines with fluorescent reporter genes driven by its promoter/enhancer in Xenopus laevis. (2010)
  • Author(s): Suzuki, K., Kashiwagi, K., Ujihara, M., Marukane, T., Tazaki, A., Watanabe, K., Mizuno, N., Ueda, Y., Kondoh, H., Kashiwagi, A., Mochii, M.
  • Journal Title: Developmental Dynamics Volume: 12 Pages: 3172-3181
  • Peer Reviewed
• [Journal Article] ベトナム人におけるAS3MT遺伝子多型とヒ素代謝能の関係 (2010)
  • Author(s): 阿草哲郎・藤原純子・竹下治男・田辺信介・岩田久人・國頭恭
  • Journal Title: DNA多型 Volume: 18 Pages: 242-245
  • Peer Reviewed
• [Journal Article] ダイエタリーサプリメント使用される大豆抽出物とエチニルエストラジオールの複合エストロゲン活性 (2010)
  • Author(s): 川添禎浩・那智和奈・石橋弘志・小原智未・有薗幸司・橋本香織・北条康司・鈴木隆
  • Journal Title: 食品衛生学雑誌 Volume: 51 Pages: 101-109
  • Peer Reviewed
• [Presentation] Molecular and functional characterization of aryl hydrocarbon receptor repressor (AHRR) from the chicken (Gallus gallus). (2011)
  • Author(s): Iwata, H., Lee, J.S., Kim, E.Y., Nomaru, K.
  • Organizer: Society of Toxicology 50^<th> anniversary annual meeting
  • Place of Presentation: Washington, D.C., USA.
  • Year and Date: 20110306-20110310
• [Presentation] Association of arsenic exposure and genetic polymorphisms with arsenic metabolism in a Vietnamese population. (2011)
  • Author(s): Agusa, T., Kunito, T., Fujiwara, J., Takeshita, T.B., Min, P.K., Viet, P.H., Tanabe, S., Iwata, H.
  • Organizer: Society of Toxicology 50^<th> anniversary annual meeting
  • Place of Presentation: Washington, D.C., USA.
  • Year and Date: 20110306-20110310
• [Presentation] 2,3,7,8-tetrachlorodibenzo-p-dioxin responsive transcripts in avian embryonic liver, identified by large-scale differential display system with vertebrate-common degenerate oligonucleotide primers. (2011)
  • Author(s): Teraoka, H., Ito, S., Ukai, N., Kim, E.Y., Iwata, H., Kitazawa, T., Hiraga, T., Endoh, D.
  • Organizer: Society of Toxicology 50^<th> anniversary annual meeting
  • Place of Presentation: Washington, D.C., USA.
  • Year and Date: 20110306-20110310
• [Presentation] ベトナム人におけるヒ素代謝能の個人差:ヒ素曝露レベルと代謝遺伝子多型の影響. (2011)
  • Author(s): 阿草哲郎・岩田久人・國頭恭・藤原純子・Minh,T.B.・Trang, P.T.K.・Viet, P.H.・竹下治男・田辺信介
  • Organizer: 第16回ヒ素シンポジウム
  • Place of Presentation: 旭川市、日本
  • Year and Date: 20110205-20110206
• [Presentation] 膠原病のポリジーンネットワーク (2011)
  • Author(s): 能勢眞人
  • Organizer: 愛媛大学プロテオ医学研究センター 第1回学術シンポジウム 難病のプロテオゲノミクス
  • Place of Presentation: 松山市、日本
  • Year and Date: 2011-02-06
• [Presentation] Transcriptional regulation of CYP1A genes in amphibians. (2010)
  • Author(s): Suzuki, T.K.,Iwata, H.
  • Organizer: 環境ホルモン学会(日本内分泌撹乱化学物質学会)第13回研究発表会
  • Place of Presentation: 東京、日本
  • Year and Date: 20101216-20101217
• [Presentation] Effects of endogenous and xenobiotic compounds on DNA binding and transactivation of the crustacean ecdysteroid receptor. (2010)
  • Author(s): Hirano, M., Ishibashi, H., Kim, E.Y., Arizono, K., Iwata, H.
  • Organizer: 環境ホルモン学会(日本内分泌撹乱化学物質学会)第13回研究発表会
  • Place of Presentation: 東京、日本
  • Year and Date: 20101216-20101217
• [Presentation] Transactivation potencies of the Baikal Seal (Pusa sibirica) peroxisome proliferator-activated receptor a by perfluoroalkyl carboxylates and sulfonates : Estimation of PFOA induction equivalency factors. (2010)
  • Author(s): Ishibashi, H., Kim, E.Y., Iwata, H.
  • Organizer: 環境ホルモン学会(日本内分泌撹乱化学物質学会)第13回研究発表会
  • Place of Presentation: 東京、日本
  • Year and Date: 20101216-20101217
• [Presentation] Characterization of the AHR/ARNT/AHRR-CYP1A signaling pathway in avian species. (2010)
  • Author(s): Iwata, H., Lee, J.S., Kim, E.Y.
  • Organizer: 環境ホルモン学会(日本内分泌撹乱化学物質学会)第13回研究発表会
  • Place of Presentation: 東京、日本
  • Year and Date: 20101216-20101217
• [Presentation] Transactivation potencies of the Baikal seal (Pusa sibirica) pregnane X receptor by persistent organic pollutants. (2010)
  • Author(s): Pham, T.T.L., Ishibashi, H., Kadota, M., Kim, E.Y., Iwata H.
  • Organizer: 環境ホルモン学会(日本内分泌撹乱化学物質学会)第13回研究発表会
  • Place of Presentation: 東京、日本
  • Year and Date: 20101216-20101217
• [Presentation] Screening of environmental chemicals using a surface plasmon resonance(SPR)biosensor in combination with constitutive androstane receptor(CAR). (2010)
  • Author(s): Pham, T.D., Ishibashi, H., Kim, E.Y., Iwata, H.
  • Organizer: 環境ホルモン学会(日本内分泌撹乱化学物質学会)第13回研究発表会
  • Place of Presentation: 東京、日本
  • Year and Date: 20101216-20101217
• [Presentation] Functional characterization of cytochrome P450 1A1, 1A2 and 1B1 from the Baikal seal(Pusa sibirica). (2010)
  • Author(s): Yamaguchi, K., Kim, E.Y., Kubota, A.,Iwata, H.
  • Organizer: 韓国環境分析学会秋季学術大会& The 2^<nd> Korea-Japan Symposium on Environmental Chemistry
  • Place of Presentation: Seoul, Korea
  • Year and Date: 20101103-20101105
• [Presentation] Chemical impacts on aquatic birds and mammals : xenobiotic receptor-based risk assessment. (2010)
  • Author(s): Iwata, H., Kim, E.Y.
  • Organizer: The 4^<th> bilateral seminar Italy-Japan, Physical and chemical impacts on marine organisms-for conservation of biodiversity and sustainability
  • Place of Presentation: Nagakute, Japan
  • Year and Date: 20101024-20101027
• [Presentation] Regulation and catalytic function of avian CYP1A: Interspecies similarities and differences. (2010)
  • Author(s): Iwata, H., Kim, E.Y., Lee, J.S., Kubota, A.
  • Organizer: 10^<th> International symposium on cytochrome P450 biodiversity and biotechnology
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 20101003-20101007
• [Presentation] Functional analyses of the mysid ecdysteroid receptor : molecular cloning, DNA binding ability and ligand-induced transactivation. (2010)
  • Author(s): Hirano, M., Ishibashi, H., Kim, E., Arizono, K., Iwata, H.
  • Organizer: 10^<th> International symposium on cytochrome P450 biodiversity and biotechnology
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 20101003-20101007
• [Presentation] Transactivation potencies of Baikal seal (Pusa sibirica) peroxisome proliferator-activated receptor a by perfluoroalkyl carboxylates and perfluoroalkyl sulfonates. (2010)
  • Author(s): Ishibashi, H., Kim, E.Y., Iwata, H.
  • Organizer: 10^<th> International symposium on cytochrome P450 biodiversity and biotechnology
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 20101003-20101007
• [Presentation] Cytochrome P450 CYP2 genes in the common cormorant : evolutionary relationships with diapsid CYP2 clan sequences and chemical effects on their expression. (2010)
  • Author(s): Kubota, A., Stegeman, J., Goldstone, J., Nelson, D., Kim, E.Y., Tanabe, S.,Iwata, H.
  • Organizer: 10^<th> International symposium on cytochrome P450 biodiversity and biotechnology
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 20101003-20101007
• [Presentation] Characterization of CYP1 genes in Xenopus laevis and Xenopus tropicalis. (2010)
  • Author(s): Suzuki, K., Iwata, H.
  • Organizer: 10^<th> International symposium on cytochrome P450 biodiversity and biotechnology
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 20101003-20101007
• [Presentation] Transcriptional activities of Baikal seal (Pusa sibirica) peroxisome proliferator-activated receptor a by perfluoroalkyl carboxylates and perfluoroalkyl sulfonates. (2010)
  • Author(s): Ishibashi, H., Kim, E.Y., Iwata, H.
  • Organizer: International symposium on modeling and analysis of marine environmental problems (MAMEP 2010)
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20100922-20100923
• [Presentation] Identification and characterization of CYP1 genes in Xenopus laevis and Xenopus tropicalis. (2010)
  • Author(s): Suzuki, K.T., Iwata, H.
  • Organizer: International symposium on modeling and analysis of marine environmental problems (MAMEP 2010)
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20100922-20100923
• [Presentation] Effects of endogenous and xenobiotic ligands on DNA binding and transactivation of the mysid ecdysteroid receptor. (2010)
  • Author(s): Hirano, M., Ishibashi, H., Kim, E.Y., Arizono, K., Iwata, H.
  • Organizer: International symposium on modeling and analysis of marine environmental problems (MAMEP 2010)
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20100922-20100923
• [Presentation] Risk assessment of dioxins and related compounds in the black-footed albatross : Application of an in vitro approach. (2010)
  • Author(s): Thuruthippallil L.M., Kim, E.Y.,Iwata, H.
  • Organizer: International symposium on modeling and analysis of marine environmental problems (MAMEP 2010)
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20100922-20100923
• [Presentation] 芳香族炭化水素受容体(AhR)の分子生物学的解析による鳥類のダイオキシン類感受性評価 (2010)
  • Author(s): 藤澤希望・池中良徳・山本秀明・金恩英・李鎮善・岩田久人・石塚真由美
  • Organizer: 第61回北海道獣医師大会・平成22年度北海道地区三学会
  • Place of Presentation: 函館市、日本
  • Year and Date: 20100909-20100910
• [Presentation] ふっ素テロマーアルコールがヒメダカ再生産に及ぼす影響 (2010)
  • Author(s): 山内良子・石橋弘志・内田雅也・孫〓富・平野将司・金俊佑・冨永伸明・有薗幸司
  • Organizer: 第16回バイオアッセイ研究会・日本環境毒性学会
  • Place of Presentation: つくば市、日本
  • Year and Date: 20100902-20100903
• [Presentation] Common and different gene loci susceptible to sialoadenitis and dacryoadenitis in a sjogren's syndrome mouse model. (2010)
  • Author(s): Kamao, T., Miyazaki, T., Soga, Y., Komori, H., Terada, M., Nose, M.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20100823-20100827
• [Presentation] A novel mouse model for primary focal segmental glomerulosclerosis developing associated with insertional mutagenesis by transgene. (2010)
  • Author(s): Tanaka, Y., Fujino, T., Terada, M., Ito, M., Shudo, M., Okabe, M., Nose, M.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20100823-20100827
• [Presentation] Allelic polymorphism of murine osteopontin implicates functional differences in antigen presentation by dendritic cells. (2010)
  • Author(s): Miyazaki, T., Tanaka, Y., Kurata, M., Ono, M., Nose, M.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20100823-20100827
• [Presentation] ベトナムのヒ素汚染地域住民におけるヒ素代謝に関与する要因 (2010)
  • Author(s): 阿草哲郎・岩田久人・藤原純子・國頭恭・竹下治男・Minh, T.B.・Trang P.T.K.・Viet, P.H.・田辺信介
  • Organizer: 第21回日本微量元素学会
  • Place of Presentation: 京都市、日本
  • Year and Date: 20100703-20100704
• [Presentation] ベトナム人のヒ素代謝プロファイルとGST遺伝子多型の関係:ヒ素暴露レベルの影響 (2010)
  • Author(s): 阿草哲郎・岩田久人・藤原純子・國頭恭・Minh, T.B.・Trang, P.T.K.・Vet, P.H.・竹下治男・田辺信介
  • Organizer: 第19回環境化学討論会
  • Place of Presentation: 春日井市、日本
  • Year and Date: 20100621-20100623
• [Presentation] Accumulation of trace elements in harp seals (Phoca groenlandica) from Pangnirtung in Baffin Island, Canada. (2010)
  • Author(s): Agusa, T., Nomura, K., Kunito, T., Anan, Y., Iwata, H., Tanabe, S.
  • Organizer: 6^<th> International Conference on Marine Pollution and Ecotoxicology
  • Place of Presentation: Hong Kong, China
  • Year and Date: 20100531-20100603
• [Presentation] Methylation of inorganic arsenic and genetic polymorphisms in arsenic (+III oxidation state) methyltransferase in a Vietnamese population. (2010)
  • Author(s): Agusa, T., Fujihara, J., Takeshita, H., Tanabe, S., Iwata, H., Kunito, T., Minh, T.B., Trang, P.T.K., Viet, P.H.
  • Organizer: The 3^<rd> International Congress on Arsenic in the Environment
  • Place of Presentation: Tainan, Taiwan
  • Year and Date: 20100517-20100521
• [Presentation] Studies on cytochrome P450 expression and function in wildlife. (2010)
  • Author(s): Iwata, H.
  • Organizer: NUTMEG 2010-28^<th> annual meeting
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 2010-10-08
• [Book] 環境毒性学3.6バイオマーカー (2011)
  • Author(s): 岩田久人
  • Total Pages: 11
  • Publisher: 朝倉書店
• [Book] 環境毒性学 7 環境毒性学の未来 (2011)
  • Author(s): 岩田久人
  • Total Pages: 5
  • Publisher: 朝倉書店
• [Book] Molecular characterization of peroxisome proliferator-activated receptor isoforms from the Baikal seal (Pusa sibirica), Interdisciplinary Studies on Environmental Chemistry Vol.4, Environmental Specimen Bank : Exploring Possibility of Setting-up ESBs in Developing Countries (2010)
  • Author(s): Ishibashi, H, Kim, E.Y., Tanabe, S., Iwata, H.
  • Total Pages: 7
  • Publisher: TERRAPUB
• [Book] Development of an in vitro reporter gene assay for screening the ecdysone receptor agonists in mysid crustacean, Interdisciplinary Studies on Environmental Chemistry Vol.4, Environmental Specimen Bank : Exploring Possibility of Setting-up ESBs in Developing Countries (2010)
  • Author(s): Hirano, M., Ishibashi, H., Kim, E.Y., Arizono, K., Iwata, H.
  • Total Pages: 7
  • Publisher: TERRAPUB
• [Book] Toxic effects of 2,3,7,8-tetrachlorodibenzo p-dioxin (TCDD) on peripheral nervous system of developing red seabream (Pagrus major) embryos, Interdisciplinary Studies on Environmental Chemistry Vol.4, Environmental Specimen Bank : Exploring Possibility of Setting-up ESBs in Developing Countries (2010)
  • Author(s): Iida, M., Kim, E.Y., Murakami, Y., Shima, Y., Iwata, H.
  • Total Pages: 6
  • Publisher: TERRAPUB
• [Book] Cytochrome P450 family 1 genes in Xenopus tropicalis, Interdisciplinary Studies on Environmental Chemistry Vol.4, Environmental Specimen Bank : Exploring Possibility of Setting-up ESBs in Developing Countries (2010)
  • Author(s): Suzuki, K.T., Iwata, H.
  • Total Pages: 6
  • Publisher: TERRAPUB
• [Book] Interindividual variation in arsenic metabolism in a Vietnamese population : association with 17 single nucleotide polymorphisms in AS3MT, Interdisciplinary Studies on Environmental Chemistry vol.3, Bioloical Responses to Contaminants : from Molecular to Community Level (2010)
  • Author(s): Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T.B., Trang, P.T.K., Viet, P.H., Tanabe, S.
  • Total Pages: 7
  • Publisher: TERRAPUB
• [Book] Risk assessment of dioxins in wild birds by the combination of contamination level and species-specific response of aryl hydrocarbon receptor, Interdisciplinary Studies on Environmental Chemistry vol.3, Biological Responses to Contaminants : from Molecular to Community Level (2010)
  • Author(s): Iwata, H., Kim, E.Y.
  • Total Pages: 8
  • Publisher: TERRAPUB
• [Book] Methylation of inorganic arsenic and genetic polymorphisms in arsenic (+III oxidation state)methyltransferase in a Vietnamese population, Arsenic in Geosphere and Human Diseases (2010)
  • Author(s): Agusa, T., Fujihara, J., Takeshita, H., Tanabe, S., Iwata, H., Kunito, T., Minh, T.B., Trang, P.T.K., Viet, P.H.
  • Total Pages: 3
  • Publisher: CRC Press
• [Book] リウマチ病学テキスト (2010)
  • Author(s): 能勢眞人
  • Total Pages: 8
  • Publisher: 診断と治癒社
• [Remarks]
  • URL: http://ecotoxiwata.jp/