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2013 Fiscal Year Final Research Report

Development of ionic liquids for bioscience

Research Project

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Project/Area Number 21225007
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Functional materials/Devices
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

OHNO Hiroyuki  東京農工大学, 工学(系)研究科(研究院), 教授 (00176968)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Nobuhumi  東京農工大学, 大学院工学研究院, 教授 (60313293)
MORISHIMA Keisuke  大阪大学大, 学院工学研究科, 教授 (60359114)
MIZUMO Tomonobu  ㈱メドレックス, 研究員 (90436676)
Project Period (FY) 2009-05-11 – 2014-03-31
Keywordsイオン液体 / タンパク質 / バイオマス / エネルギー変換 / 細胞 / 機能制御
Research Abstract

Many novel ionic liquids have been synthesized to support several different fields of bio-science. Following four subjects have been carried out in this project. (1) We have succeeded to synthesize novel ionic liquids (ILs) to dissolve biomass and extract useful materials such as cellulose. (2) We have proposed new methods to detect material conversion of such a cellulose into other chemicals in ILs as well as changes in molecular weight and molecular weight distribution of the extracted polysacchar ides in the ILs. Then (3) ethanol, one of products from biomass, was used to generate electricity with the aid of enzymes in suitably-designed ILs. (4) Design of ILs as a protection fluid for cells has also been carried out to keep bio-activity of cells under air. Long term stability is a future task.

  • Research Products

    (49 results)

All 2014 2013 2012 2011 2010 2009 Other

All Journal Article (25 results) (of which Peer Reviewed: 25 results) Presentation (23 results) (of which Invited: 5 results) Remarks (1 results)

  • [Journal Article] 1H NMR analysis of cellulose dissolved in non-deuterated ionic liquids2014

    • Author(s)
      K. Kuroda, H. Kunimura, Y. Fukaya, and H. Ohno
    • Journal Title

      Cellulose

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Effect of amines as activators on the alcohol-oxidizing activity of pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase2014

    • Author(s)
      K. Takeda, T. Ishida, K. Igarashi, M. Samejima, N. Nakamura, H. Ohno
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Dissolution of wet wood biomass without heating2014

    • Author(s)
      M. Abe, T. Yamada, and H. Ohno
    • Journal Title

      RSC Advances

      Volume: 4 Pages: 17136-17140

    • DOI

      10.1039/C4RA01038H

    • Peer Reviewed
  • [Journal Article] The two-step electrochemical oxidation of alcohols using a novel recombinant PQQ alcohol dehydrogenase as a catalyst for a bioanode2013

    • Author(s)
      K. Takeda, H. Matsumura, T. Ishida, M. Samejima, K. Igarashi, N. Nakamura, and H. Ohno
    • Journal Title

      Bioelectrochem

      Volume: 94 Pages: 75-78

    • DOI

      10.1016/j.bioelechem.2013.08.001

    • Peer Reviewed
  • [Journal Article] Direct HPILC analysis on cellulose depolymerisation in ionic liquids2013

    • Author(s)
      K. Kuroda, Y. Fukaya and H. Ohno
    • Journal Title

      Analytical Methods

      Volume: 5 Pages: 3172-3176

    • DOI

      10.1039/c3ay40182k

    • Peer Reviewed
  • [Journal Article] Phosphonium phosphonate-type zwitterion/ water mixture showing variable hydrogen bonding ability as a function of temperature2013

    • Author(s)
      Y. Fukaya and H. Ohno
    • Journal Title

      PhysChemChemPhys

      Volume: 15 Pages: 14941-14944

    • DOI

      10.1039/c3cp52178h

    • Peer Reviewed
  • [Journal Article] A paradoxical method for NAD+/NADH accumulation on an electrode surface using a hydrophobic ionic liquid2013

    • Author(s)
      M. Masuda, Y. Motoyama, J. Kuwahara, N. Nakamura, and H. Ohno
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 39 Pages: 334-337

    • DOI

      10.1016/j.bios.2012.07.051

    • Peer Reviewed
  • [Journal Article] Electrocatalytic reduction of oxygen by bilirubin oxidase in hydrophobic ionic liquids containing a small quantity of water2013

    • Author(s)
      J. Kuwahara, R. Ikari, K. Murata, N. Nakamura, and H. Ohno
    • Journal Title

      Catalysis Today

      Volume: 200 Pages: 49-53

    • DOI

      10.1016/j.cattod.2012.06.009

    • Peer Reviewed
  • [Journal Article] Detection of small differences in the hydrophilicity of ions using LCST-type phase transition of an ionic liquid-water mixture2013

    • Author(s)
      S. Saita, Y. Kohno, and H. Ohno
    • Journal Title

      Chem. Commun

      Volume: 49 Pages: 93-95

    • DOI

      10.1039/c2cc37006a

    • Peer Reviewed
  • [Journal Article] Hydrophobic and polar ionic liquids2013

    • Author(s)
      Y. Fukaya and H. Ohno
    • Journal Title

      PhysChemChemPhys

      Volume: 15 Pages: 4066-4072

    • DOI

      10.1039/c3cp44214d

    • Peer Reviewed
  • [Journal Article] Cold crystallisation behaviour of water molecules in ionic liquids as a screening method to evaluate biocompatibility of the hydrated ionic liquids2013

    • Author(s)
      K. Fujita, Y. Nikawa, and H. Ohno
    • Journal Title

      Chem. Comm

      Volume: 49 Pages: 3257-3259

    • DOI

      10.1039/c3cc39033k

    • Peer Reviewed
  • [Journal Article] Ionic liquid/water mixtures : From hostility to conciliation2012

    • Author(s)
      Y. Kohno and H. Ohno
    • Journal Title

      Chem. Commun

      Volume: 48 Pages: 7119-7130

    • DOI

      10.1039/c2cc31638b

    • Peer Reviewed
  • [Journal Article] Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid2012

    • Author(s)
      T. Hoshino, K. Fujita, A. Higashi, K. Sakiyama, H. Ohno, and K. Morishima
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 427 Pages: 379-384

    • DOI

      10.1016/j.bbrc.2012.09.068

    • Peer Reviewed
  • [Journal Article] Selective transport of water-soluble proteins from aqueous to ionic liquid phase via a temperature-sensitive phase change of these mixtures2012

    • Author(s)
      Y. Kohno, N. Nakamura, and H. Ohno
    • Journal Title

      Australian J. Chem

      Volume: 65 Pages: 1548-1553

    • DOI

      10.1071/CH12282

    • Peer Reviewed
  • [Journal Article] Insect biofuel cells using trehalose included in insect hemolymph leading to an insect-mountable biofuel cell2012

    • Author(s)
      K. Shoji, Y. Akiyama, M. Suzuki, T. Hoshino, N. Nakamura, H. Ohno, and K. Morishima
    • Journal Title

      Biomed. Microdevices

      Volume: 14 Pages: 1063-1068

    • DOI

      10.1007/s10544-012-9706-z

    • Peer Reviewed
  • [Journal Article] Fast and facile dissolution of cellulose with tetrabutylphosphonium hydroxide containing 40 wt% water2012

    • Author(s)
      M. Abe, Y. Fukaya, and H. Ohno
    • Journal Title

      Chem. Commun

      Volume: 48 Pages: 1808-1810

    • DOI

      10.1039/c2cc16203b

    • Peer Reviewed
  • [Journal Article] "Cytochrome c dissolved in 1-allyl-3-methylimidazolium chloride type ionic liquids undergoes a quasi-reversible redox reaction up to 140 ºC2012

    • Author(s)
      K. Tamura, N. Nakamura, and H. Ohno
    • Journal Title

      Biotech. and Bioeng

      Volume: 109 Pages: 729-735

    • DOI

      10.1002/bit.24357

    • Peer Reviewed
  • [Journal Article] Temperature-responsive ionic liquid/water interfaces : relation between hydrophilicity of ions and dynamic phase change2012

    • Author(s)
      Y. Kohno and H. Ohno
    • Journal Title

      PCCP

      Volume: 14 Pages: 5063-5070

    • DOI

      10.1039/c2cp24026b

    • Peer Reviewed
  • [Journal Article] Electrochemical analysis of electrodeimmobilized dehydrogenases in hydrated choline dihydrogen phosphate-type ionic liquid2011

    • Author(s)
      K. Fujita, N. Nakamura, K. Murata, K. Igarashi, M. Samejima, and H. Ohno
    • Journal Title

      Electrochim. Acta

      Volume: 56 Pages: 7224-7227

    • DOI

      10.1016/j.electacta.2011.03.107

    • Peer Reviewed
  • [Journal Article] High performance "ionic liquid"chromatography2011

    • Author(s)
      Y. Fukaya, A. Tsukamoto, K. Kuroda, and H. Ohno
    • Journal Title

      Chem. Commun

      Volume: 47 Pages: 1994-1996

    • DOI

      10.1039/c0cc05307d

    • Peer Reviewed
  • [Journal Article] Extraction of proteins with temperature sensitive and reversible phase change of ionic liquid/water mixture2011

    • Author(s)
      Y. Kohno, S. Saita, K. Murata, N. Nakamura, and H. Ohno
    • Journal Title

      Polym. Chem

      Volume: 2 Pages: 862-867

    • DOI

      10.1039/c0py00364f

    • Peer Reviewed
  • [Journal Article] Extraction of polysaccharides from bran with phosphonate- or phosphinate-derived ionic liquids under short mixing time and low temperature2010

    • Author(s)
      M. Abe, Y. Fukaya, and H. Ohno
    • Journal Title

      Green Chem

      Volume: 12 Pages: 1274-1280

    • DOI

      10.1039/c003976d

    • Peer Reviewed
  • [Journal Article] Task specific ionic liquids for cellulose technology2009

    • Author(s)
      H. Ohno and Y. Fukaya
    • Journal Title

      Chem. Lett

      Volume: 38 Pages: 2-7

    • DOI

      10.1246/cl.2009.2

    • Peer Reviewed
  • [Journal Article] Biocatalytic oxidation of cellobiose in hydrated ionic liquid2009

    • Author(s)
      K. Fujita, N. Nakamura, K. Igarashi, M. Samejima and H. Ohno
    • Journal Title

      Green Chem

      Volume: 11 Pages: 351-354

    • DOI

      10.1039/b813529k

    • Peer Reviewed
  • [Journal Article] Direct electrochemistry of bilirubin oxidase on three-dimensional gold nanoparticle electrodes and its application in a biofuel cell2009

    • Author(s)
      K. Murata, K. Kajiya, N. Nakamura, and H. Ohno
    • Journal Title

      Energy Environ. Sci

      Volume: 2 Pages: 1280-1285

    • DOI

      10.1039/B912915D

    • Peer Reviewed
  • [Presentation] イオン液体中のセルロースの分解反応のメカニズム解析2013

    • Author(s)
      黒田浩介、深谷幸信、大野弘幸
    • Organizer
      第4回イオン液体討論会
    • Place of Presentation
      慶應義塾大学 神奈川県
    • Year and Date
      2013-11-21
  • [Presentation] Phase behaviour of ionic liquid/molecular liquid mixture (PL01)2013

    • Author(s)
      H. Ohno and Y. Kohno
    • Organizer
      33rd International Conference on Solution Chemistry
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2013-07-08
  • [Presentation] Progress of Ionic Liquids as Important Partners for Biopolymers (PL-4)2013

    • Author(s)
      H. Ohno
    • Organizer
      Congress of European Polymer Federation
    • Place of Presentation
      Pisa, Italy
    • Year and Date
      2013-06-18
  • [Presentation] An enzymatic biofuel cell using hydrophobic ionic liquids as electrolytes (P330)2013

    • Author(s)
      M. Masuda, N. Nakamura and H. Ohno
    • Organizer
      COIL5
    • Place of Presentation
      Vilamoura, Algarve, Portugal
    • Year and Date
      2013-04-23
  • [Presentation] Ionic Liquids : Potential Partner For Biorefinery2013

    • Author(s)
      H. Ohno
    • Organizer
      POLYCHAR 21 World Forum on Advanced Materials
    • Place of Presentation
      Gwangju, Korea
    • Year and Date
      2013-03-12
  • [Presentation] イオン液体に溶解したセルロースのNo-D NMR 法を用いた解析(P60)2012

    • Author(s)
      國村治仁、黒田浩介、深谷幸信、大野弘幸
    • Organizer
      第3回イオン液体討論会
    • Place of Presentation
      沖縄
    • Year and Date
      2012-12-08
  • [Presentation] Water-containing novel solvent for extracting cellulose from wood biomass without heating (O-02)2012

    • Author(s)
      M. Abe, Y. Fukaya, and H. Ohno
    • Organizer
      The 3rd International Cellulose Conference
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2012-10-10
  • [Presentation] Electrochemical Oxidation of Glucose by Nitroxide Radicals or Gold Nanoparticles in Ionic Liquids (3661)2012

    • Author(s)
      A. Konno, M. Abe, and H. Ohno
    • Organizer
      Pacific Rim Meeting on Electrochemical and Solid State Science 2012
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2012-10-07
  • [Presentation] Temperature sensitive phase transition of ionic liquid/water mixtures for bioprocesses2012

    • Author(s)
      H. Ohno
    • Organizer
      15th International Biotechnology Symposium
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2012-09-20
    • Invited
  • [Presentation] Biomass treatment with polar ionic liquids2012

    • Author(s)
      H. Ohno
    • Organizer
      3rd Asian-Pacific Conference on Ionic Liquids and Green Processes (APCIL'12)
    • Place of Presentation
      Beijing, China
    • Year and Date
      2012-09-17
  • [Presentation] Toward the marriage between ionic liquid based biorefinery and electrochemistry (KL-7)2012

    • Author(s)
      H. Ohno
    • Organizer
      EUCHEM 2012, (Molten salts and ionic liquids XXIV conference)
    • Place of Presentation
      Celtic Manor, Wales, England
    • Year and Date
      2012-08-08
  • [Presentation] Reversible and dynamic phase change of ionic liquid/water mixtures2012

    • Author(s)
      H. Ohno
    • Organizer
      5th Australian Symposium on Ionic Liquids
    • Place of Presentation
      Monash University, Australia
    • Year and Date
      2012-05-03
  • [Presentation] Design and Application of ionic liquids to show temperature-sensitive LCST-type phase transition after mixing with water (BBB3.7)2012

    • Author(s)
      H. Ohno and Y. Kohno
    • Organizer
      Materials Research Society, 2012 Spring meeting
    • Place of Presentation
      San Francisco, CA, USA
    • Year and Date
      2012-04-11
    • Invited
  • [Presentation] Insect-mountable biofuel cell with self-circulation system2012

    • Author(s)
      K. Shoji, Y. Akiyama, M. Suzuki, T. Hoshino, N. Nakamura, H. Ohno, and K. Morishima
    • Organizer
      Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference
    • Place of Presentation
      USA
    • Year and Date
      2012-01-29
  • [Presentation] 持続性社会に貢献するイオン液体(1Sp04)2011

    • Author(s)
      大野弘幸
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-26
    • Invited
  • [Presentation] イオン液体を用いた次世代バイオファイナリー(S2)2011

    • Author(s)
      大野弘幸
    • Organizer
      第18回セルロース学会
    • Place of Presentation
      信州大学、長野
    • Year and Date
      2011-07-14
    • Invited
  • [Presentation] Electrochemical oxidation of glucose in ionic liquids with gold nano-particle modified electrode (244)2011

    • Author(s)
      M. Abe, Y. Fukaya, H, Ohno
    • Organizer
      4th International Congress on Ionic Liquids
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2011-06-17
  • [Presentation] Temperature sensitive phase separation of ionic liquid/water mixture for extraction of proteins (LMP 69)2010

    • Author(s)
      Y. Kohno and H. Ohno
    • Organizer
      EUCHEM 2010 Conference on Molten Salts and Ionic Liquids
    • Place of Presentation
      Bamberg, Germany
    • Year and Date
      20100314-19
  • [Presentation] Polar ionic liquids for a sustainable world (#92-13)2010

    • Author(s)
      H. Ohno
    • Organizer
      International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
    • Place of Presentation
      Convention Center, Honolulu, Hawaii.
    • Year and Date
      2010-12-15
  • [Presentation] LCST 挙動を示すイオン液体/水混合系を用いたタンパク質の抽出(2Pa079)2010

    • Author(s)
      税田祥平、河野雄樹、大野弘幸
    • Organizer
      第59回高分子討論会
    • Place of Presentation
      北海道大学
    • Year and Date
      2010-09-16
  • [Presentation] 水和イオン液体中におけるセロビオースの酵素酸化反応の電気化学的追跡(2H6-47)2010

    • Author(s)
      藤田恭子、中村暢文、五十嵐圭日子、鮫島正浩、大野弘幸
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学本部キャンパス
    • Year and Date
      2010-03-27
  • [Presentation] Functional design of ionic liquids for biomass energy conversion2009

    • Author(s)
      H. Ohno
    • Organizer
      Regional Conference on Ionic Liquids
    • Place of Presentation
      Kuala Lumpur, Malaysia
    • Year and Date
      2009-11-24
    • Invited
  • [Presentation] Playing Games with Ionic Liquids and Polymer Materials (Oral 13)2009

    • Author(s)
      H. Ohno
    • Organizer
      3rd International Congress on Ionic Liquids (COIL3)
    • Place of Presentation
      Cairns Australia
    • Year and Date
      2009-06-02
  • [Remarks]

    • URL

      http://www.tuat.ac.jp/~ohno/

URL: 

Published: 2015-06-25  

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