2013 Fiscal Year Final Research Report
Regulatory Mechanisms of Meiosis in Fission Yeast
| Project/Area Number |
21227007
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (S)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Kazusa DNA Research Institute (2012-2013)
The University of Tokyo (2009-2011) |
|---|
Principal Investigator |
YAMAMOTO Masayuki 公益財団法人かずさＤＮＡ研究所, その他部局等, 特別客員研究員 (40114706)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YAMASHITA Akira かずさDNA研究所, ヒトゲノム研究部, 室長 (30312276)
SATO Masamitsu 早稲田大学, 先進理工学部, 准教授 (50447356)
|
|---|
| Project Period (FY) |
2009-05-11 – 2014-03-31
|
| Keywords | 遺伝学 / 発生・分化 / 減数分裂 / 情報伝達 / RNA / 微小管 |
|---|
| Research Abstract |
We investigated the regulatory mechanisms of meiosis in fission yeast. Certain mRNAs required for meiosis are transcribed but undergo degradation called "selective elimination" in vegetatively growing cells. We analyzed this mechanism and identified a number of molecules involved. A pivotal factor Mmi1 protein recognized and bound to specific hexanucleotide motifs on the target mRNAs. meiRNA, which inactivates Mmi1 to promote meiosis, carried numerous copies of the hexanucleotide motifs and lured Mmi1. Meanwhile, we found that phosphorylation of Ser-2 residues on the C-terminal domain (CTD) of RNA polymerase II was essential to transcribe a specific gene required for the initiation of meiosis, and that the stress-responsive MAP kinase pathway was involved in this regulation.
|
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Mmi1 RNA surveillance machinery directs RNAi complex RITS to selective meiotic genes in fission yeast2012
Author(s)
E. Hiriart, A. Vavasseur, L. Touat- Todeschini, A. Yamashita, B. Gilquin, E. Lambert, J. Perot, Y. Shichino, N. Nazaret, C. Boyault, J. Lachuer, D. Perazza, M. Yamamoto and A. Verdel
-
Journal Title
EMBO J
Volume: 31
Pages: 2296-2308
Peer Reviewed
-
-
[Journal Article] Transient structure associated with the spindle pole body directs meiotic microtubule reorganization in S. pombe2012
Author(s)
C. Funaya, S. Samarasinghe, S. Pruggnaller, M. Ohta, Y. Connolly, J. Muller, H. Murakami, A. Grallert, M. Yamamoto, D. Smith, C. Antony and K. Tanaka
-
Journal Title
Curr. Biol
Volume: 22
Pages: 562-574
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
