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2013 Fiscal Year Final Research Report

Direct visualization of the in vivo distribution of chemical components in plant cells by TOF-SIMS

Research Project

  • PDF
Project/Area Number 21228004
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Wood science
Research InstitutionNagoya University

Principal Investigator

FUKUSHIMA Kazuhiko  名古屋大学, 生命農学研究科, 教授 (80222256)

Co-Investigator(Kenkyū-buntansha) IMAI Takanori  名古屋大学, 大学院生命農学研究科, 准教授 (20252281)
MATSUSHITA Yasuyuki  名古屋大学, 大学院生命農学研究科, 准教授 (60335015)
Co-Investigator(Renkei-kenkyūsha) KURODA Katsushi  独立行政法人森林総合研究所, 木材特性領域, 主任研究員 (90399379)
AOKI Dan  名古屋大学, 大学院生命農学研究科, 助教 (80595702)
Project Period (FY) 2009-05-11 – 2014-03-31
Keywords組織構造・材形成 / リグニン / 抽出成分・微量成分 / 保存・木質文化
Research Abstract

A complex analytical system (cryo-TOF-SIMS/SEM) was developed to achieve a series of experiment (surface preparation, cryo-TOF-SIMS, and cryo-SEM) for quick-frozen samples. A repetition of the surface preparation and measurements make three-dimensional analysis possible. The method provides a microscopic visualization of 3D distribution for water-soluble components in living plants.

  • Research Products

    (47 results)

All 2014 2013 2012 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 14 results) Presentation (23 results) (of which Invited: 10 results) Book (3 results) Remarks (4 results)

  • [Journal Article] Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part I2014

    • Author(s)
      P. Zheng, D. Aoki, M. Yoshida, Y. Matsushita, T. Imai, K. Fukushima
    • Journal Title

      Microscopic investigation by POM, UV microscopy, and TOF-SIMS. Holzforschung

    • DOI

      10.1515/hf-2013-0231

    • Peer Reviewed
  • [Journal Article] Aluminum localization in the cell walls of the mature xylem of maple tree detected by elemental imaging using time-of-flight secondary ion mass spectrometry (TOF-SIMS)2014

    • Author(s)
      K. Saito, Y. Watanabe, Y. Matsushita, T. Imai, T. Koike, Y, Sano, R. Funada, K. Fukazawa, K. Fukushima
    • Journal Title

      Holzforschung

      Volume: 68 Pages: 85-92

    • Peer Reviewed
  • [Journal Article] Adsorption behavior of poly(dimethyl-diallylammonium chloride) on pulp fiber studied by cryo time-of-flight secondary ion mass spectrometry and cryo-scanning electron microscopy2014

    • Author(s)
      T. Masumi, Y. Matsushita, D. Aoki, R. Takama, K. Saito, K. Kuroda, K. Fukushima
    • Journal Title

      Appl. Surf. Sci.

      Volume: 289 Pages: 155-159

    • Peer Reviewed
  • [Journal Article] The accumulation pattern of ferruginol in the heartwood-forming Cryptomeria japonica xylem as determined by time-of-flight secondary ion mass spectrometry and quantity analysis2014

    • Author(s)
      K. Kuroda, T. Fujiwara, K. Hashida, T. Imai, M. Kushi, K. Saito, K. Fukushima
    • Journal Title

      Annals of Botany

      Volume: 113 Pages: 1029-1036

    • Peer Reviewed
  • [Journal Article] Topochemical analysis of organic/inorganic components in frozen samples by cryo-TOF-SIMS/SEM system2013

    • Author(s)
      D. Aoki, K. Fukushima
    • Journal Title

      Cellulose Commun.

      Volume: 20 Pages: 188-192

  • [Journal Article] Distribution of Inorganic Elements in Heartwood and Sapwood of Hinoki Cypres Detected by TOF-SIMS2013

    • Author(s)
      K. Saito, T. Mitsutani, Y. Matsushita, T. Imai, K. Fukushima
    • Journal Title

      Mokuzai Gakkaishi

      Volume: 59 Pages: 353-360

    • Peer Reviewed
  • [Journal Article] Development of the Cryo-TOF-SIMS/SEM System and Application for Topochemical Analysis of Plant Biomolecules2013

    • Author(s)
      D. Aoki, K. Kuroda, Y. Hanaya, K. Saito, R. Takama, Y. Matsushita, K. Fukushima
    • Journal Title

      Mokuzai Gakkaishi

      Volume: 59 Pages: 367-374

    • Peer Reviewed
  • [Journal Article] The Cryo-TOF-SIMS/SEM System for analysis of the chemical distribution in freeze-fixed Cryptomeria japonica wood2013

    • Author(s)
      K. Kuroda, T. Fujiwara, T. Imai, R. Takama, K. Sato, Y. Matsushita, K. Fukushima
    • Journal Title

      Surface and Interface Analysis

      Volume: 45 Pages: 215-219

    • Peer Reviewed
  • [Journal Article] Evaluation of white radish sprouts growth influenced by magnetic fields using TOF-SIMS and MCR2013

    • Author(s)
      S. Aoyagi, K. Kuroda, R. Takama, K. Fukushima, I. Kayano, S. Mochizuki, A. Yano
    • Journal Title

      Surface and Interface Analysis

      Volume: 45(1) Pages: 264-267

    • Peer Reviewed
  • [Journal Article] Fragmentation mechanism of the phenylcoumaran-type lignin model compound by ToF-SIMS2013

    • Author(s)
      Y. Matsushita, K. Ioka , K. Saito , R. Takama , D. Aoki, K. Fukushima
    • Journal Title

      Holzforschung

      Volume: 67 Pages: 365-370

    • Peer Reviewed
  • [Journal Article] Molecular Imaging of Chemical Compounds in Plant Tissue by Time-of-flight Secondary Ion mass Spectrometry2013

    • Author(s)
      K. Saito, K. Fukushima
    • Journal Title

      生物物理学会誌

      Volume: 53 Pages: 24-27

  • [Journal Article] Distribution of extracts including 4,8-dihydroxy-5-methoxy-2naphthaldehyde in Diospyros kaki analyzed by gas chromatography-mass spectrometry and time-of-flight secondary ion mass spectrometry2012

    • Author(s)
      Y. Matsushita, I-C. Jang, T. Imai, R. Takama, K. Saito, T. Masumi, S-C. Lee, K. Fukushima
    • Journal Title

      Holzforschung

      Volume: 66 Pages: 705-709

    • Peer Reviewed
  • [Journal Article] Direct mapping of morphological distribution of syringyl and guaiacyl lignin in the xylem of maple by time-of-flight secondary ion mass spectrometry2012

    • Author(s)
      K. Saito, Y. Watanabe, M. Shirakawa, Y. Matsushita, T. Imai, T. Koike, Y. Sano, R. Funada, K. Fukazawa, K. Fukushima
    • Journal Title

      The Plant Journal

      Volume: 69(3) Pages: 542-552

    • Peer Reviewed
  • [Journal Article] Distribution analysis of triglyceride having repair effect on damaged human hair by TOF-SIMS2012

    • Author(s)
      T. Kojima, S. Tsuji, M. Niwa, K. Saito, Y. Matsushita, K. Fukushima
    • Journal Title

      International Journal of Polymer Analysis and characterization

      Volume: 17(1) Pages: 21-28

    • Peer Reviewed
  • [Journal Article] Application of TOF-SIMS to the direct determination of syringyl to guaiacyl (S/G) ratio of lignin2011

    • Author(s)
      K. Saito, T. Kishimoto, Y. Matsushita, T. Imai, K. Fukushima
    • Journal Title

      Surface and Interface Analysis

      Volume: 43(1-2) Pages: 281-284

    • Peer Reviewed
  • [Journal Article] Imaging analysis of cosmetic ingredients interacted with human hair using TOF-SIMS2011

    • Author(s)
      T. Kojima, H. Kitano, M. Niwa, K, Saito, Y. Matsushita, K. Fukushima
    • Journal Title

      Surface and Interface Analysis

      Volume: 43(1-2) Pages: 562-565

    • Peer Reviewed
  • [Journal Article] 木質バイオマスのTOF-SIMSによる解析2009

    • Author(s)
      齋藤香織 , 福島和彦
    • Journal Title

      Cellulose Communications

      Volume: 16(4) Pages: 161-165

  • [Presentation] Distribution of lignin in ray parenchyma cells from the sapwood to heartwood of Pinus densiflora2014

    • Author(s)
      P. Zheng, D. Aoki, Y. Matsushita, S. Yagami, K. Fukushima
    • Organizer
      第64回日本木材学会大会
    • Place of Presentation
      松山
    • Year and Date
      20140313-15
  • [Presentation] パルプ洗浄工程における洗浄剤の効果2014

    • Author(s)
      浅井龍太郎, 青木弾, 石黒正雄, 松下泰幸, 八神祐絵 , 福島和彦
    • Organizer
      第64回日本木材学会大会
    • Place of Presentation
      松山
    • Year and Date
      20140313-15
  • [Presentation] Chemical mapping of tree biomolecules by the cryo-TOF-SIMS/SEM system2013

    • Author(s)
      Y. Hanaya, D. Aoki, R. Takama, Y. Matsushita, K. Kuroda, K. Fukushima
    • Organizer
      Annual Meeting of International Academy of Wood Science
    • Place of Presentation
      Nanjing, China
    • Year and Date
      20131017-21
  • [Presentation] Current status and future plans of the preliminary examination of the Category 3 particles of the Hayabusa-returned samples2013

    • Author(s)
      M. Uesugi, H. Naraoka, F. Kitajima, M. Ito, H. Yabuta, Y. Takano, H. Mita, K. Fukushima, D. Aoki, I. Ohnishi, Y. Kebukawa, T. Yada, Y. Karouji, Y. Ishibashi, T. Okada, M. Abe
    • Organizer
      Hayabusa 2013: Symposium of solar system materials
    • Place of Presentation
      Sagamihara, Japan
    • Year and Date
      20131016-18
  • [Presentation] Distribution of lignin in ray cells from the sapwood to heartwood of Pinus densiflora Sieb. et Zucc2013

    • Author(s)
      P. Zheng, D. Aoki, M. Yoshida, Y. Matsushita, S. Yagami, T. Imai, K. Fukushima
    • Organizer
      第63回日本木材学会大会
    • Place of Presentation
      盛岡
    • Year and Date
      20130327-29
  • [Presentation] Direct mapping of morphological distribution of syringyl and guaiacyl lignin in the xylem of maple by TOF-SIMS and development of cryo-TOF-SIMS/SEM system2013

    • Author(s)
      K. Fukushima, K. Saito, D. Aoki, Y. Matsushita, K. Kuroda
    • Organizer
      Carbo Summit 2013
    • Place of Presentation
      Zauchensee
    • Year and Date
      20130317-21
  • [Presentation] CryoTOF-SIMS/SEMシステムによる植物生体成分の分子マッピングの試み2012

    • Author(s)
      白河学、黒田克史、渡辺陽子、渡辺誠、小池孝良、齋藤香織、松下泰幸、今井貴規、高間瑠佳、福島和彦
    • Organizer
      第62回日本木材学会大会
    • Place of Presentation
      北海道
    • Year and Date
      20120315-17
  • [Presentation] TOF-SIMSを用いた抄紙薬品の吸着挙動解析 -アルケニルコハク酸(ASA)の吸着挙動について-2012

    • Author(s)
      真清高志,松下泰幸,今井貴規,福島和彦
    • Organizer
      第62回日本木材学会大会
    • Place of Presentation
      北海道
    • Year and Date
      20120315-17
  • [Presentation] Application of TOF-SIMS to the direct determination of plant components and introduction of Cryo TOF-SIMS/SEM system2012

    • Author(s)
      K. Fukushima, K. Saito and K. Kuroda
    • Organizer
      The 12th International Symposium on Biomimetic Materials Processing
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      20120124-27
    • Invited
  • [Presentation] TOF-SIMS:樹木抽出成分生合成・分布の可視化2012

    • Author(s)
      今井貴規
    • Organizer
      日本木材学会組織と材質研究会2012秋季シンポジウム、木材の形成機構解明に向けた新規イメージング技術、 pp.5-8
    • Place of Presentation
      東京農工大学府中キャンパス
    • Year and Date
      2012-09-26
    • Invited
  • [Presentation] Analysis of lignin structure using ToF-SIMS2012

    • Author(s)
      K. Ioka, Y. Matsushita, T. Imai, K. Saito, K. Fukushima
    • Organizer
      Twelfth International Symposium on Biomimetic Materials Processing
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2012-01-24
  • [Presentation] Direct determination of lignin using ToF-SIMS2011

    • Author(s)
      K. Fukushima, K. Saito
    • Organizer
      The 11th International Symposium on Biomimetic Materials Processing (BMMP-11)
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      20110125-28
    • Invited
  • [Presentation] Application of TOF-SIMS to the direct determination of plant components and introduction of Cryo TOF-SIMS/SEM complex apparatus2011

    • Author(s)
      K. Fukushima, K. Saito, K. Kuroda
    • Organizer
      The International Symposium on SIMS and Related Techniques Based on Ion-Solid Interactions (SISS-13)
    • Place of Presentation
      Aichi, Japan
    • Year and Date
      2011-06-23
    • Invited
  • [Presentation] TOF-SIMSを用いた広葉樹におけるリグニン S/G比分布の解析2010

    • Author(s)
      齋藤香織,渡邊陽子,松下泰幸,今井貴規,船田良,深澤和三,福島和彦
    • Organizer
      第60回日本木材学会
    • Place of Presentation
      宮崎
    • Year and Date
      20100317-19
  • [Presentation] TOF-SIMSの心材形成研究への導入2010

    • Author(s)
      福島和彦、齋藤香織
    • Organizer
      日本木材学会組織と材質研究会2010秋のシンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2010-09-14
    • Invited
  • [Presentation] Cryo-SEMとToF-SIMSでみた心材形成2010

    • Author(s)
      黒田克史
    • Organizer
      日本木材学会組織と材質研究会2010秋のシンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2010-09-14
    • Invited
  • [Presentation] ToF-SIMS の生体試料への適用2010

    • Author(s)
      福島和彦
    • Organizer
      アルバックファイ技術講演会
    • Place of Presentation
      京都
    • Year and Date
      2010-06-04
    • Invited
  • [Presentation] ToF-SIMS の生体試料への適用2010

    • Author(s)
      福島和彦
    • Organizer
      アルバックファイ技術講演会
    • Place of Presentation
      東京
    • Year and Date
      2010-06-02
    • Invited
  • [Presentation] 木材科学の可能性を広げる基礎科学の重要性2010

    • Author(s)
      福島和彦
    • Organizer
      一般社団法人日本木材学会設立記念シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-05-15
    • Invited
  • [Presentation] Imaging analysis of cosmetic ingredients interacted with human hair using TOF-SIMS2009

    • Author(s)
      T. Kojima, H. Kitano, M. Niwa, K. Saito, Y. Matsushita, K. Fukushima
    • Organizer
      SIMS XVII
    • Place of Presentation
      Tronto
    • Year and Date
      20090914-18
  • [Presentation] Application of TOF-SIMS to the direct determination of syringyl to guaiacyl (S/G) ratio of lignin2009

    • Author(s)
      K. Saito, T. Kishimoto, Y. Matsushita, T. Imai, K. Fukushima
    • Organizer
      SIMS XVII
    • Place of Presentation
      Tronto
    • Year and Date
      20090914-18
  • [Presentation] TOF-SIMSによるヒノキ心材成分の分子マッピング2009

    • Author(s)
      齋藤香織,吉田正人,今井貴規,松下泰幸,福島和彦
    • Organizer
      第59回日本木材学会
    • Place of Presentation
      松本
    • Year and Date
      20090315-17
  • [Presentation] The chemical analysis of plant cell wall using ToF-SIMS2009

    • Author(s)
      K. Fukushima
    • Organizer
      The 3rd Wood Science & Biotechnology Symposium
    • Place of Presentation
      Chonnam National University
    • Year and Date
      2009-11-20
    • Invited
  • [Book] 第2章5. TOF-SIMSを用いたリグニンの構造解析と分布の可視化 , In:"リグニン利用の最新動向"2013

    • Author(s)
      福島和彦
    • Total Pages
      40-47
    • Publisher
      シーエムシー出版
  • [Book] 木質の形成バイオマス科学への招待第2版2011

    • Author(s)
      福島和彦ら編著
    • Total Pages
      307-315, 317-320, 351-353, 550-552
    • Publisher
      海青社
  • [Book] グリーンバイオケミストリーの最前線2010

    • Author(s)
      福島和彦
    • Total Pages
      51-61
    • Publisher
      シーエムシー出版
  • [Remarks] 名古屋大学大学院生命農学研究科森林化学研究分野

    • URL

      http://forestchem.sakura.ne.jp/

  • [Remarks] 名古屋大学

    • URL

      http://www.nagoya-u.ac.jp/about-nu/public-relations/researchinfo/upload_images/20120202_agr.pdf

  • [Remarks] (環境展望台)(独)国立環境研究所

    • URL

      http://tenbou.nies.go.jp/news/jnews/detail.php?i=7536

  • [Remarks] マイナビニュース

    • URL

      http://news.mynavi.jp/news/2012/02/03/010/

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Published: 2015-07-16  

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