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2013 Fiscal Year Final Research Report

Mechanisms and physiological roles of electro-chemical Coupling mediated by voltage-sensor domain proteins

Research Project

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Project/Area Number 21229003
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionOsaka University

Principal Investigator

YASUSHI Okamura  大阪大学, 医学(系)研究科(研究院), 教授 (80201987)

Co-Investigator(Kenkyū-buntansha) SAKIMURA Kenji  新潟大学, 脳研究所, 教授 (40162325)
YOSHIDA Manabu  東京大学, 大学院理学系研究科, 准教授 (60301785)
NAKAGAWA Atsushi  大阪大学, たんぱく質研究所, 教授 (20188890)
FUJIWARA Yuichiro  大阪大学, 大学院医学系研究科, 准教授 (20532980)
TSUTSUI Hidekazu  大阪大学, 大学院医学系研究科, 特任講師 (30392038)
OUKOUCHI Yoshifumi  大阪大学, 大学院医学系研究科, 助教 (90435818)
Co-Investigator(Renkei-kenkyūsha) MIYAWAKI Atsushi  理化学研究所, 細胞機能探索技術開発チーム, シニア・リーダー (80251445)
Project Period (FY) 2009-05-11 – 2014-03-31
Keywords生体膜 / チャネル / トランスポーター / 能動輸送
Research Abstract

Molecular mechanisms of voltage sensor domain proteins, including voltage-sensing phosphatase and voltage-gated proton channels, were studied by X-ray crystallography, electrophysiology, fluorescence imaging or fluorometry. We also exploited advantages of molecular mechanisms to develop efficient voltage-reporter protein and applied them in zebrafish heart and mouse auditory cortex and were convinced that these probes serve as efficient reporter. Knock out mice were establsihed for VSP, VSOP/Hv1 and VSOP2 and physiological functions of these voltage-signaling molecules have been revealed.

  • Research Products

    (31 results)

All 2014 2013 2012 2011 2010 2009 Other

All Journal Article (19 results) Presentation (6 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Rapid evaluation of a protein-based voltage probe using a field-induced membrane potential change2014

    • Author(s)
      Tsutsui H, Jinno Y, Tomita A & Okamura Y
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1838(7) Pages: 1730-7

    • DOI

      10.1016/j.bbamem.2014.03.002

  • [Journal Article] X-ray crystal structure of voltage-gated proton channel2014

    • Author(s)
      Takeshita K, Sakata S, Yamashita E, Fujiwara Y, Kawanabe A, Kurokawa T, Okochi Y, Matsuda M, Narita H, Okamura Y & Nakagawa A
    • Journal Title

      Nat. Strucl. & Mol. Biol.

      Volume: (4) Pages: 352-7

    • DOI

      10.1038/nsmb.2783

  • [Journal Article] PLC-mediated PI(4,5)P2 hydrolysis regulates activation and inactivation of TRPC6/7 channels2014

    • Author(s)
      Itsuki K, Imai Y, Hase H, Okamura Y, Inoue R & Mori M
    • Journal Title

      J. Gen. Physiol

      Volume: 143(2) Pages: 183-201

  • [Journal Article] Phosphatase activity of the voltage-sensing phosphatase, VSP, shows graded dependence on the extent of activation of the voltage sensor2014

    • Author(s)
      Sakata S & Okamura Y
    • Journal Title

      J. Physiol.

      Volume: 592(Pt 5) Pages: 899-914

    • DOI

      10.1113/jphysiol.2013.263640

  • [Journal Article] Mapping of sites facing aqueous environment of voltage-gated proton channel at resting state: A study with PEGylation protection2013

    • Author(s)
      Kurokawa T & Okamura Y
    • Journal Title

      Biochem. Biophys. Acta

      Volume: 1838(1 Pt B) Pages: 382-7

    • DOI

      10.1016/j.bbamem.2013.10.001

  • [Journal Article] Potential role of voltage-sensing phosphatases in regulation of cell structure through the production of PI(3,4)P22013

    • Author(s)
      Yamaguchi S, Kurokawa T, Taira I, Aoki N, Sakata S, Okamura Y & Homma KJ
    • Journal Title

      J. Cell Physiol.

      Volume: 229(4) Pages: 422-33

    • DOI

      10.1002/jcp.24463

  • [Journal Article] Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase2013

    • Author(s)
      Tsutsui H, Jinno Y, Tomita A, Niino Y, Yamada Y, Mikoshiba K, Miyawaki A & Okamura Y
    • Journal Title

      J. Physiol. , Epub 2013 Jul 8

      Volume: 591(Pt 18) Pages: 4427-37

    • DOI

      10.1113/jphysiol.2013.257048

  • [Journal Article] Optically detected structural change in the N-terminal region of the voltage-sensor domain2013

    • Author(s)
      Tsutsui H, Jinno Y, Tomita A & Okamura Y
    • Journal Title

      Biophys. J.

      Volume: 105(1) Pages: 108-15

  • [Journal Article] Structural characteristics of the redox-sensing coiled coil in the voltage-gated H+ channel2013

    • Author(s)
      Fujiwara Y, Takeshita K, Nakagawa A & Okamura Y
    • Journal Title

      J. Biol. Chem.

      Volume: 288(25) Pages: 17968-75

  • [Journal Article] The cytoplasmic coiled-coil mediates cooperative gating temperature sensitivity in the voltage-gated H+ channel Hv12012

    • Author(s)
      Fujiwara Y, Kurokawa T, Takeshita K, Kobayashi M, Okochi Y, Nakagawa A & Okamura Y
    • Journal Title

      Nat. Communi.

      Volume: 3 Pages: 816

    • DOI

      10.1038/ncomms1823

  • [Journal Article] Autoimmune disorder phenotypes in HVCN1 gene deficient mice2012

    • Author(s)
      Sasaki M, Tojo A, Okochi Y, Miyawaki N, Kamimura D, Yamaguchi A, Murakami M & Okamura Y
    • Journal Title

      Biochem. J.

      Volume: 450(2) Pages: 295-301

  • [Journal Article] Gating of the Designed Trimeric/Tetrameric Voltage-Gated H+ Channel2012

    • Author(s)
      Fujiwara Y, Kurokawa T, Takeshita K, Nakagawa A, Larsson HP & Okamura Y
    • Journal Title

      J. Physiol.

      Volume: 591(Pt 3) Pages: 627-40

  • [Journal Article] 3' phosphatase activity toward PI(3,4)P2 by voltage-sensing phosphatase2012

    • Author(s)
      Kurokawa T, Takasuga S, Sakata S, Yamaguchi S, Horie S, Homma KJ, Sasaki T & Okamura Y
    • Journal Title

      VSP. Proc. Natl. Acad. Sci. U.S.A.

      Volume: 109(25) Pages: 10089-94

    • DOI

      10.1073/pnas.1203799109

  • [Journal Article] Crystal structure of the cytoplasmic PTEN-like region of Ci-VSP provides insight into substrate specificity and redox regulation of the phosphoinositide phosphatase activity2011

    • Author(s)
      Matsuda M, Takeshita K, Kurokawa T, Sakata S, Suzuki M, Yamashita E, Okamura Y & Nakagawa A
    • Journal Title

      J. Biol. Chem.

      Volume: 286(26) Pages: 23368-77

  • [Journal Article] Coupling of the phosphatase activity of Ci-VSP to its voltage sensor activity over the entire range of voltage sensitivity2011

    • Author(s)
      Sakata S, Hossain MI & Okamura Y
    • Journal Title

      J. Physiol.

      Volume: 589(11) Pages: 2687-705

  • [Journal Article] Voltage-sensing phosphatase: its molecular relationship with PTEN2011

    • Author(s)
      Okamura Y & Dixon JE
    • Journal Title

      Physiology

      Volume: 26(1) Pages: 6-13

  • [Journal Article] Visualizing voltage dynamics in zebrafish heart2010

    • Author(s)
      Tsutsui H, Higashijima S, Miyawaki A & Okamura Y
    • Journal Title

      J. Physiol.

      Volume: 588(12) Pages: 2017-21

  • [Journal Article] VSOP/Hv1 proton channels sustain calcium entry, neutrophil migration, and superoxide production by limiting cell depolarization and acidification2010

    • Author(s)
      El Chemaly A, Okochi Y, Sasaki M, Arnaudeau S, Okamura Y & Demaurex N
    • Journal Title

      J. Exp. Med.

      Volume: 207(1) Pages: 129-39

  • [Journal Article] Functionality of the voltage-gated proton channel truncated in S42010

    • Author(s)
      Sakata S, Kurokawa T, Norholm MH, Takagi M, Okochi Y, von Heijne G & Okamura Y
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A.

      Volume: 107(5) Pages: 2313-8

    • DOI

      10.1073/pnas.0911868107

  • [Presentation] 電位依存性プロトンチャネルVSOP1/H1の動作原理2014

    • Author(s)
      岡村康司 藤原祐一郎 川鍋陽 坂田宗平 黒川竜紀
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島県鹿児島市
    • Year and Date
      2014-03-18
  • [Presentation] Voltage-sensor domain proteins: phosphonositide signal, proton permeation and molecular tools2014

    • Author(s)
      Okamura Y
    • Organizer
      Biophysical Society 58th Annual Meetingシンポジウム
    • Place of Presentation
      米国サンフランシスコ
    • Year and Date
      2014-02-17
  • [Presentation] Voltage-sensing phosphatase -it's structure and portability2013

    • Author(s)
      Sakata S, Okamura Y, Tsutsui H
    • Organizer
      The 1st Awaji International Workshop on Electron Spin Science & Technology: Biological and Materials Sciences Oriented Applications
    • Place of Presentation
      兵庫県淡路市
    • Year and Date
      2013-06-17
  • [Presentation] How excited to talk with PI?: Lesson from voltage-sensing phosphatase.2013

    • Author(s)
      Okamura Y
    • Organizer
      日本生理学会第90回大会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-29
  • [Presentation] Secret lives of voltage-sensing phosphatases,"Bioelectrochemistry"2012

    • Author(s)
      Okamura Y
    • Organizer
      ゴードンカンファレンス
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      2012-07-03
  • [Presentation] Gating Mechanisms of Voltage-Gated Proton Channels2012

    • Author(s)
      Okamura Y
    • Organizer
      ゴードンカンファレンス
    • Place of Presentation
      米国ベンチュラ
    • Year and Date
      2012-02-20
  • [Book] Voltage-gated proton channels.Comprehensive Biophysics ed. Montal M.2013

    • Author(s)
      Okamura Y
    • Publisher
      Elsevier
  • [Book] 生物学辞典第五版2013

    • Author(s)
      岡村康司, 分担執筆及び分担編集
    • Publisher
      岩波書店
  • [Book] Voltage-gated ion channels and novel sensing proteins. Handbook of Neurochemistry and Molecular Neurobiology: Neural Signaling Mechanisms: ed. Mikoshiba K.2009

    • Author(s)
      Okamura Y
    • Publisher
      Springer
  • [Remarks]

    • URL

      http://resou.osaka-u.ac.jp/ja/research/2014/20140303_1

  • [Patent(Industrial Property Rights)] 電位依存性プロトンチャネルポリペプチドおよびその利用2011

    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      特許第4887484号
    • Acquisition Date
      2011-12-22
  • [Patent(Industrial Property Rights)] 新規イオンチャネル様ポリペプチドおよびその利用2011

    • Inventor(s)
      佐藤矩行・岡村康司・岩崎広英・村田喜理
    • Industrial Property Rights Holder
      佐藤矩行・岡村康司・岩崎広英・村田喜理
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      特許第4802331号
    • Acquisition Date
      2011-08-19

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Published: 2015-07-16   Modified: 2019-02-08  

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