2013 Fiscal Year Final Research Report
Chromatin regulation by lncRNA
| Project/Area Number |
21241046
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OHTA Kunihiro 東京大学, 総合文化研究科, 教授 (90211789)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Takatomi 東京大学, 大学院・総合文化研究科, 助教 (30451850)
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| Co-Investigator(Renkei-kenkyūsha) |
KUGOU Kazuto 東京大学, 大学院・総合文化研究科, 特任助教 (60554425)
HIROTA Kouji 首都大学, 東京理工学研究科, 教授 (00342840)
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| Project Period (FY) |
2009-04-01 – 2014-03-31
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| Keywords | ノンコーディングRNA / クロマチン / ヒストン修飾 / 転写制御 / ゲノム発現 |
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| Research Abstract |
We previously identified a lncRNA involved in chromatin and gene regulation of fission yeast fbp1 gene. This lncRNA (mlonRNA, metabolic stress induced lncRNA) is transcribed from the far upstream of fbp1 gene overlapping with the coding segment. In response to glucose starvation, cascade transcription of mlonRNAs occur to cause stepwise chromatin remodeling in the fbp1 promoter, followed by massive induction of mRNA.
We here revealed histone modification is locally modulated by mlonRNA transcription and some chromatin modifying proteins. Chromatin binding of Atf1, which is necessary for mlonRNA transcription, is enhanced by mlonRNA transcription, thereby forming a positive feedback loop. We also discovered that mlonRNA is transported into cytoplasms to bind polysomes. Since mlonRNA harbors many uORFs, mlonRNA does not have little coding potential. RNA Seq analysis revealed 42 mlonRNA-like lncRNAs as well as antisense lncRNAs. We also detected mlonRNA-like lncRNA in mice cultured cells.
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Research Products
(45 results)
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[Journal Article] The role of stress-activated Sty1 and Gcn2 kinases and proto-oncoprotein homologue Int6/eIF3e in responses to endogenous oxidative stress during histidine starvation2010
Author(s)
Nemoto N., Udagawa T., Ohira T., Jiang L., Hirota K., Wilkinson C., Bahler J., Jones N., Ohta K., Wek R., Asano K
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Journal Title
J. Mol. Biol
Volume: 404
Pages: 183-201
Peer Reviewed
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[Journal Article] Rec8 guides canonical Spo11 distribution along yeast meiotic chromosomes2009
Author(s)
Kugou K., Yamada S., Itoh S., Fukuda T., Sasanuma H., Mori S., Katou Y., Itoh T., Matsumoto K., Shibata T., Shirahige K., and Ohta K
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Journal Title
Mol. Biol. Cell
Volume: 20
Pages: 3064-3076
Peer Reviewed
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