Systematic research of stable isotopic fractionation and equilibrate effects during metabolisms of extremophiles

2011 Fiscal Year Final Research Report

• Project/Area Number: 21244077
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Geology
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: TAKAI Ken 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, プログラムディレクター (80359166)
• Co-Investigator(Kenkyū-buntansha): UENO Yu-ichiro 東京工業大学, 大学院・理工学研究科, 准教授 (90422542)
• Project Period (FY): 2009 – 2011
• Keywords: 地球史 / 微生物活動 / 水素代謝 / 硫黄代謝 / 質量非依存分別

Research Abstract

We completed the experiments for effect of H2-consuming and. producing metabolisms on stable hydrogen isotopic equilibrium, effect of S0-reducing metabolisms on multiple sulfur isotopic equilibrium and fractionation and effect of sulfur-disproportionating metabolisms on multiple sulfur isotopic fractionation. All the data have provided very new insights into understanding the systematics of stable isotopic fractionation and equilibrate effects during metabolisms of extremophiles. Several research papers are now under preparation.

Research Products

• [Journal Article] Seasonal change in microbial sulfur cycling in monomictic Lake Fukami-ike, Japan. (2012)
  • Author(s): Nakagawa, M., Ueno, Y., Hattori, S., Umemura, M., Yagi, A., Takai, K., Koba, K., Sasaki, Y., Makabe, A., and Yoshida, N.
  • Journal Title: Limnol. Oceanography Volume: (in press)
  • Peer Reviewed
• [Journal Article] Sulfur metabolisms in epsilon-and gamma-Proteobacteria in deep-sea hydrothermal fields. (2011)
  • Author(s): Yamamoto, M., and Takai, K.
  • Journal Title: Front. Microbiol. Volume: 2 Pages: 192
  • DOI: doi:10.3389/fmicb.2011.00192
  • Peer Reviewed
• [Journal Article] Isotopic variation of molecular hydrogen in 20o-375oC hydrothermal fluids as detected by a new analytical method (2010)
  • Author(s): Kawagucci, S., Toki, T., Ishibashi, J., Takai, K., Ito, M., Oomori, T., and Gamo, T.
  • Journal Title: J. Geophys. Res. Volume: 115 Pages: G03021
  • Peer Reviewed
• [Journal Article] Molecular characterization of inorganic sulfur-compounds metabolisms in a deep-sea epsilon-proteobacterium Sulfurovum sp. (2010)
  • Author(s): Yamamoto, M., Nakagawa, S., Shimamura, S., Takai, K., and Horikoshi, K.
  • Journal Title: NBC37-1.Environ. Volume: 12 Pages: 1144-1153
  • Peer Reviewed
• [Presentation] Hydrogen isotope systematics of H2-H2O-CH4 during hydrogenotrophic methanogenesis (2011)
  • Author(s): Kobayashi, M., Kawagucci, S., Hattori, S., Yamada, K., Ueno, Y., Takai, K., Yoshida, N.
  • Organizer: AGU Fall Meeting 2011
  • Place of Presentation: Moscone Center, San Francisco, California
  • Year and Date: 2011-12-10
• [Presentation] メタンの4つの水素はすべて水に由来するのか:H2-H2O-CH4の水素同位体システマティクスから考える水素資化メタン生成代謝におけるメタンの水素同位体比決定機構 (2011)
  • Author(s): 川口慎介,小林真理子,服部祥平,山田桂太,上野雄一郎,高井研,吉田尚弘
  • Organizer: 日本微生物生態学会第27回大会
  • Place of Presentation: 京都大学,京都
  • Year and Date: 2011-10-08
• [Presentation] Novel sulfur disproportionation of a deep-sea vent thermophile (2011)
  • Author(s): Takai, K.
  • Organizer: International Union of Microbiology Societies Congress 2011
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan
  • Year and Date: 2011-09-08
• [Presentation] H2-H2O-CH4の水素同位体システマティクスから考える微生物由来メタンの水素同位体比決定機構の解明 (2011)
  • Author(s): 小林真理子,川口慎介,服部祥平,山田桂太,上野雄一郎,高井研,吉田尚弘
  • Organizer: 2011年度日本地球化学会
  • Place of Presentation: 北海道大学,札幌
  • Year and Date: 2011-09-06
• [Presentation] Novel sulfur disproportionation of a deep-sea vent thermophile (2010)
  • Author(s): Hasegawa, H., Yamamoto, M., Nunoura, T., Kawagucci, S., Ueno, Y., Takai, K.
  • Organizer: Goldschmidt 2010
  • Place of Presentation: Knoxville Convention Center, Tennessee
  • Year and Date: 2010-06-08
• [Presentation] 化学合成生態系におけるH2消費を安定同位体比を用いて評価する (2010)
  • Author(s): 川口慎介,高井研
  • Organizer: 地球惑星科学連合2010年大会
  • Place of Presentation: 幕張メッセ,千葉
  • Year and Date: 2010-05-24