2012 Fiscal Year Final Research Report
Molecular basis of the action of Hfq-binding small RNA inEscherichia coli.
| Project/Area Number |
21247025
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
AIBA Hiroji 鈴鹿医療科学大学, 薬学部, 教授 (20025662)
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| Project Period (FY) |
2009 – 2012
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| Keywords | 小分子 RNA / Hfq / サイレンシング / 転写後制御 / 大腸菌 |
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| Research Abstract |
Molecular basis of the function of Hfq-binding sRNA has been studied using SgrS as a model. The major findings are as follows. (1) The 14 nt region within SgrS is the minimum region required for base-pairing and translational repression of ptsG mRNA. (2) The region between 711 and 750 of RNase E is sufficient for the functional interaction with Hfq to support the rapid degradation of ptsG mRNA. (3) The polyU tail of rho-independent terminator is essential for Hfq action. (4) The functional Hfq-binding module of bacterial sRNAs consists of a double or single hairpin preceded by a U-rich sequence and followed by a 3’ poly(U) tail. (5) The synthetic Hfq-binding small RNAs to knock down desired mRNAs have been successfully designed.
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