Novel functions of nuclear transport factors : stress-response mechanism of cell nucleus

2011 Fiscal Year Final Research Report

• Project/Area Number: 21247032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: YONEDA Yoshihiro 大阪大学, 生命機能研究科, 教授 (80191667)
• Co-Investigator(Renkei-kenkyūsha): OKA Masahiro 大阪大学, 生命機能研究科, 助教 (40432504)
• Project Period (FY): 2009 – 2011
• Keywords: 核輸送 / ストレス応答 / importinα / 細胞核 / 遺伝子発現 / 細胞死

Research Abstract

Various cellular stresses induce the nuclear accumulation of importinα. However, it is unknown whether accumulated importinαperforms roles in the nucleus. In this study, we found that nuclear-retained importinα2binds with DNase I-sensitive nuclear component(s) and exhibits selective up-regulation of mRNA encoding Serine/ threonine kinase35(STK35). Chromatin immunoprecipitation and promoter analysis demonstrated that importinα2binds to the promoter region of STK35and accelerates its transcription. Furthermore, constitutive overexpression of STK35proteins enhances caspase-independent cell death under oxidative stress conditions. These results reveal that nuclear-localized importinα2influences gene expression and contributes directly to cell fate outcomes including non-apoptotic cell death. This is the first evidence showing that importinαhas novel functions other than nuclear protein import in the nucleus.

Research Products

• [Journal Article] The interaction between importin-alpha and Nup153promotes importin-alpha/ beta-mediated nuclear import (2012)
  • Author(s): Ogawa, Y., Miyamoto, Y., Oka, M. and Yoneda, Y
  • Journal Title: Traffic Volume: (in press)
  • Peer Reviewed
• [Journal Article] Intrinsic and extrinsic negative regulators of nuclear protein transport processes (2012)
  • Author(s): Sekimoto, T. and Yoneda, Y
  • Journal Title: Genes Cells Volume: (in press)
  • Peer Reviewed
• [Journal Article] The small GTPase Ran and Ran-binding proteins (2012)
  • Author(s): Nagai, M. and Yoneda, Y
  • Journal Title: BioMol. Concepts Volume: (in press)
  • Peer Reviewed
• [Journal Article] Nuclear importinαand its physiological importance (2012)
  • Author(s): Miyamoto, Y., Loveland, K. L. and Yoneda, Y
  • Journal Title: Commun. Integ. Biol. Volume: 5 Pages: 220-222
  • URL: http://dx.doi.org/10.1038/emboj.2011.360
  • Peer Reviewed
• [Journal Article] The role of Rae1 in Nup98-fusion mediated leukemogenesis (2011)
  • Author(s): Oka, M. and Yoneda, Y
  • Journal Title: Cell Cycle Volume: 10 Pages: 12
  • URL: http://dx.doi.org/10.4161/cc.10.12.15759
  • Peer Reviewed
• [Journal Article] Nucleo-cytoplasmic transport of microRNAs and related small RNAs (2011)
  • Author(s): Katahira, J. and Yoneda, Y
  • Journal Title: Traffic Volume: 12 Pages: 1468-1474
  • DOI: doi:10.1111/j.1600-0854.2011.01211.x
  • Peer Reviewed
• [Journal Article] Nuclear retention of importinαcoordinates cell fate through changes in gene expression (2011)
  • Author(s): Yasuda, Y., Miyamoto, Y., Yamashiro, T., Asally, M., Masui, A., Loveland, K. L. and Yoneda, Y
  • Journal Title: EMBO J. Volume: 31 Pages: 83-94
  • DOI: doi:10.1038/emboj.2011.360
  • Peer Reviewed
• [Journal Article] Cell type-dependent gene regulation by Staufen2in conjunction with Upf1 (2011)
  • Author(s): Miki, T., Kurono, S., Kaneko, Y., Katahira, J. and Yoneda, Y
  • Journal Title: BMC Mol. Biol. Volume: 12 Pages: 48
  • DOI: Doi:10.1186/1471-2199-12-48
  • Peer Reviewed
• [Journal Article] Stimulation of CK2-dependent Grp94phosphorylation by the nuclear localization signal peptide (2011)
  • Author(s): Miyata, Y., Yoneda, Y. and Yahara, I
  • Journal Title: Mol. Cell. Biochem. Volume: 356 Pages: 191-200
  • DOI: DOI:10.1007/s11010-011-0944-9
  • Peer Reviewed
• [Journal Article] Cross talk between distinct nuclear import pathways enables efficient nuclear import of E47in conjunction with its partnertranscription factors (2011)
  • Author(s): Mehmood, R., Yasuhara, N., Fukumoto, M., Oe, S., Tachinbana, T and Yoneda, Y
  • Journal Title: Mol. Biol. Cell Volume: 22 Pages: 3715-3724
  • Peer Reviewed
• [Journal Article] Cell type-specific transcriptional regulation of the gene encoding importin-α (2011)
  • Author(s): Kamikawa, Y., Yasuhara, N. and Yoneda, Y
  • Journal Title: Exp. Cell Res. Volume: 317 Pages: 1970-1978
  • URL: http://dx.doi.org/10.1016/j.yexcr.2011.05.024
  • Peer Reviewed
• [Journal Article] Targeted disruption of one of the importinαfamily members leads to female functional incompetence in delivery (2011)
  • Author(s): Moriyama, T., Nagai, M., Oka, M., Ikawa, M., Okabe, M. and Yoneda, Y
  • Journal Title: FEBS J. Volume: 278 Pages: 1561-1572
  • Peer Reviewed
• [Journal Article] Generation and characterization of a monoclonal antibody against importinα7/ NPI-2 (2011)
  • Author(s): Mizuguchi, C., Moriyama, T. and Yoneda, Y
  • Journal Title: Hybridoma Volume: 30 Pages: 307-309
  • DOI: doi:10.1089/hyb.2011.0006
  • Peer Reviewed
• [Journal Article] Generation of rat monoclonal antibody specific for mouse importinα8 (2011)
  • Author(s): Moriyama, T., Mizuguchi, C. and Yoneda, Y
  • Journal Title: Hybridoma Volume: 30 Pages: 303-306
  • DOI: doi:10.1089/hyb.2011.0004
  • Peer Reviewed
• [Journal Article] Camoretti-Mercado, B. and Solway, J. Nuclear import of serum response factor in airway smooth mascle (2011)
  • Author(s): McConville, J. F., Fernandes, D. J., Churchill, J., Dewundara, S., Kogut, P., Shah, S., Fuchs, G., Kedainis, D., Bellam, S. K., Patel, N. M., McCauley, J., Dulin, N. O., Gupta, M. P., Adam, S., Yoneda, Y
  • Journal Title: Am J. Respir. Cell Mol. Biol. Volume: 45 Pages: 453-458
  • DOI: doi:10.1165/rcmb.2008-0393OC
  • Peer Reviewed
• [Journal Article] Importinαprotein acts as a negative regulator for Snail protein nuclear import (2011)
  • Author(s): Sekimoto, T., Miyamoto, Y., Arai, S. and Yoneda, Y
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 15126-15131
  • DOI: doi:10.1074/jbc.M110.213579
  • Peer Reviewed
• [Journal Article] Proteomic analysis of importinα-interacting proteins in adult mouse brain (2011)
  • Author(s): Fukumoto, M., Sekimoto, T. and Yoneda, Y
  • Journal Title: Cell Struct. Funct. Volume: 36 Pages: 57-67
  • DOI: doi:10.1247/csf.10026
  • Peer Reviewed
• [Journal Article] Mice lacking Ran Binding Protein 1 are viable and show male infertility (2011)
  • Author(s): Nagai, M., Moriyama, T., Mehmood, R., Tokuhiro, K., Ikawa, M., Okabe, M., Tanaka, H. and Yoneda, Y
  • Journal Title: FEBS Lett. Volume: 585 Pages: 791-796
  • URL: http://dx.doi.org/10.1016/j.febslet.2011.02.002
  • Peer Reviewed
• [Journal Article] Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importinβ (2011)
  • Author(s): Ohara, R., Hata, K., Yasuhara, N., Mehmood, R., Yoneda, Y., Nakagawa, M. and Yamashita, T
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 405 Pages: 697-702
  • DOI: 10.1016/j.bbrc.2011.01.108
  • Peer Reviewed
• [Journal Article] NUP358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure during skeletal myogensis (2011)
  • Author(s): Asally, M., Yasuda, Y., Oka, M., Otsuka, S., Yoshimura, S. H., Takeyasu, K. and Yoneda, Y
  • Journal Title: FEBS J. Volume: 278 Pages: 610-621
  • DOI: doi:10.1111/j.1742-4658.2010.07982.x
  • Peer Reviewed
• [Journal Article] Specific monoclonal antibody against the nuclear pore complex protein, Nup96 (2010)
  • Author(s): Mizuguchi, C., Oka, M., Moriyama, T., Tachibana, T. and Yoneda, Y
  • Journal Title: Hybridoma Volume: 29 Pages: 551-553
  • DOI: doi:10.1089/hyb.2011.0006
  • Peer Reviewed
• [Journal Article] Cytoplasmic polyadenylation element-like sequences are involved in dendritic targeting of BDNF mRNA in hippocampal neurons (2010)
  • Author(s): Oe, S. and Yoneda, Y
  • Journal Title: FEBS Lett. Volume: 584 Pages: 3424-3430
  • URL: http://dx.doi.org/10.1016/j.febslet.2010.06.040
  • Peer Reviewed
• [Journal Article] Effective culture conditions for the induction of pluripotent stem cells (2010)
  • Author(s): Okada, M., Oka, M. and Yoneda, Y
  • Journal Title: Biochim. Biophys. Acta-General Subjects Volume: 1800 Pages: 956-963
  • URL: http://dx.doi.org/10.1016/j.bbagen.2010.04.004
  • Peer Reviewed
• [Journal Article] Identification of importinαl as a novel constituent of RNA stress granules (2010)
  • Author(s): Fujimura, K., Suzuki, T., Yasuda, Y., Murata, M., Katahira, J. and Yoneda, Y
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research Volume: 1803 Pages: 865-871
  • URL: http://dx.doi.org/10.1016/j.bbamcr.2010.03.020
  • Peer Reviewed
• [Journal Article] The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export (2010)
  • Author(s): Oka, M., Asally, M., Yasuda, Y., Tachibana, T. and Yoneda, Y
  • Journal Title: Mol. Biol. Cell Volume: 21 Pages: 1885-1896
  • DOI: doi:10.1091/mbc.E09-12-1041
  • Peer Reviewed
• [Journal Article] Two isoforms of Npap60(Nup50) differentially regulate nuclear protein import (2010)
  • Author(s): Ogawa, Y., Miyamoto, Y., Asally, M., Oka, M., Yasuda, Y. and Yoneda, Y
  • Journal Title: Mol. Biol. Cell Volume: 21 Pages: 630-638
  • DOI: doi:10.1091/mbc.E09-05-0374
  • Peer Reviewed
• [Journal Article] Roles of the TREX complex in nuclear export of mRNA (2009)
  • Author(s): Katahira, J. and Yoneda, Y
  • Journal Title: RNA Biol. Volume: 6 Pages: 149-152
  • URL: http://dx.doi.org/10.1016/j.bbagrm.2011.12.001
  • Peer Reviewed
• [Journal Article] The role of the nuclear transport system in cell differentiation (2009)
  • Author(s): Yasuhara, N., Oka, M. and Yoneda, Y
  • Journal Title: Semi. Cell Dev. Biol. Volume: 20 Pages: 590-599
  • URL: http://dx.doi.org/10.1016/j.semcdb.2009.05.003
  • Peer Reviewed
• [Journal Article] A high-resolution structure of the pre-microRNA nuclear export machinery (2009)
  • Author(s): Okada, C., Yamashita, E., Lee, S. J., Shibata, S., Katahira, J., Nakagawa, A., Yoneda, Y. and Tsukihara, T
  • Journal Title: Science Volume: 326 Pages: 1275-1279
  • Peer Reviewed
• [Journal Article] Microscopic dissection of the process of stress granule assembly (2009)
  • Author(s): Fujimura, K., Katahira, J., Kano, F., Yoneda, Y. and Murata, M
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research Volume: 1793 Pages: 1728-1737
  • Peer Reviewed
• [Journal Article] Synergistic nuclear import of NeuroD1 and its partner transcription factor, E47, via heterodimerization (2009)
  • Author(s): Mehmood, R., Yasuhara, N., Oe, S., Nagai, M. and Yoneda, Y
  • Journal Title: Exp. Cell Res. Volume: 315 Pages: 1639-1652
  • URL: http://dx.doi.org/10.1016/j.yexcr.2009.02.025
  • Peer Reviewed
• [Journal Article] Selective localization of PCBP2 to cytoplasmic processing bodies (2009)
  • Author(s): Fujimura, K., Katahira, J., Kano, F., Yoneda, Y. and Murata, M
  • Journal Title: Biochim. Biophys. Acta Volume: 1793 Pages: 878-887
  • URL: http://dx.doi.org/10.1016/j.bbamcr.2009.02.002
  • Peer Reviewed
• [Journal Article] Adaptor Aly and co-adaptor Thoc5function in the Tap-p15-mediated nuclear export of HSP70mRNA (2009)
  • Author(s): Katahira, J., Inoue, H., Hurt, E. and Yoneda, Y
  • Journal Title: EMBO J. Volume: 28 Pages: 556-567
  • DOI: doi:10.1038/emboj.2009.5
  • Peer Reviewed
• [Journal Article] mDia2shuttles between the nucleus and the cytoplasm through the Importin-α/β-and CRM1-mediated nuclear transport mechanism (2009)
  • Author(s): Miki, T., Okawa, K., Sekimoto, T., Yoneda, Y., Watanabe, S., Ishizaki, T. and Narumiya, S
  • Journal Title: J. Biol. Chem. Volume: 284 Pages: 99-104
  • DOI: doi:10.1074/jbc.M806191200
  • Peer Reviewed
• [Journal Article] Infrared laser-mediated gene induction in targeted single cells in vivo (2009)
  • Author(s): Kamei, Y., Suzuki, M., Watanabe, K., Fujimoto, K., Kawasaki, T., Deguchi, T., Yoneda, Y., Todo, T., Takagi, S., Funatsu, T. and Yuba, S
  • Journal Title: Nature Methods Volume: 6 Pages: 79-81
  • Peer Reviewed
• [Presentation] The nucleocytoplasmic transport system regulates the stem cell differentiation (2011)
  • Author(s): Yasuhara, N., Yamagishi, R., Arai, Y., Yanagida, T., Kaneko, H. and Yoneda, Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20111213-16
• [Presentation] Role of nucleocytoplasmic shuttling of estrogen receptor (2011)
  • Author(s): Moriyama, T., Oka, M. and Yoneda, Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20111213-16
• [Presentation] The mechanism to determine a nuclear transport pathway by NLS sequence (2011)
  • Author(s): Kimoto, C., Yasuhara, N. and Yoneda, Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 20111213-16
• [Presentation] Importin alpha mediates biased translocation through nuclear pores in conjunction with Nup153 (2011)
  • Author(s): Ogawa, Y., Miyamoto, Y., Oka, M. and Yoneda, Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20111213-16
• [Presentation] The involvement of small GTPase Ran in cellular aging (2011)
  • Author(s): 永井理博、米田悦啓
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20111213-16
• [Presentation] Nuclear transport machineries and cell function (2011)
  • Author(s): Yoneda, Y.
  • Organizer: 6^<th> Asian-Pacific Organization for Cell Biology Congress
  • Place of Presentation: Manila(Philippines)
  • Year and Date: 20110225-28
• [Presentation] 核-細胞質間分子輸送から高次生命機能を見る (2011)
  • Author(s): 米田悦啓
  • Organizer: 第19回臨床医科学フォーラム
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2011-09-17
• [Presentation] 低分子量GTPase Ranが関与する細胞老化制御 (2010)
  • Author(s): 永井理博、安田善也、米田悦啓
  • Organizer: BMB2010
  • Place of Presentation: Kobe
  • Year and Date: 20101207-10
• [Presentation] Targeted disruption of one of the importinτfamily members leads to female functional incompetence in delivery (2010)
  • Author(s): Moriyama, T., Nagai, M., Oka, M. and Yoneda, Y.
  • Organizer: BMB2010
  • Place of Presentation: Kobe
  • Year and Date: 20101207-10
• [Presentation] Nuclear import of E47and its partner transcription factors involves across talk between distinct nuclear import pathways (2010)
  • Author(s): Mehmood, R., Yashuhara, N., and Yoneda, Y.
  • Organizer: BMB2010
  • Place of Presentation: Kobe
  • Year and Date: 20101207-10
• [Presentation] Nucleo-cytoplasmic shuttling of Oct-4 (2010)
  • Author(s): 岡正啓、盛山哲嗣、浅利宗弘、米田悦啓
  • Organizer: BMB2010
  • Place of Presentation: Kobe
  • Year and Date: 20101207-10
• [Presentation] pre-microRNA核外輸送複合体のX線結晶構造解析 (2010)
  • Author(s): 山下栄樹、慈幸千真理、李守宰、柴田理志、片平じゅん、中川敦史、米田悦啓、月原冨武
  • Organizer: BMB2010
  • Place of Presentation: Kobe
  • Year and Date: 20101207-10
• [Presentation] Nuclear protein import machinery and cell differentiation (2010)
  • Author(s): Yoneda, Y.
  • Organizer: BMB2010
  • Place of Presentation: Kobe
  • Year and Date: 20101207-10
• [Presentation] Nuclear-localized importin alpha regulates gene expression in mammalian spermatogenesis (2010)
  • Author(s): Miyamoto, Y., Yasuda, Y., Yamashiro, T., Nagai, M., Masui, A., Yoneda, Y., Jans, D. A. and Loveland, K.
  • Organizer: OzBio2010. 12^<th> IUBMB and 21^<st> FAOBMB
  • Place of Presentation: Melbourne(Australia)
  • Year and Date: 20100926-1001
• [Presentation] Nucleocytoplasmic transport and cell differentiation (2010)
  • Author(s): Yoneda, Y.
  • Organizer: OzBio2010. 12^<th> IUBMB and 21^<st> FAOBMB
  • Place of Presentation: Melbourne(Australia)
  • Year and Date: 20100926-1001
• [Presentation] 核-細胞質間蛋白質輸送から見た高次生命機能 (2010)
  • Author(s): 米田悦啓
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 20100922-24
• [Presentation] Targeted disruption of the nuclear import adaptor, importinα5, leads to female genital hypoplasia (2010)
  • Author(s): Moriyama, T., Nagai, M., Oka, M. and Yoneda, Y
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 20100519-21
• [Presentation] Transcriptional regulation of importin alpha1 in mouse embryonic stem cells (2010)
  • Author(s): Kamikawa, Y., Yasuhara, N. and Yoneda, Y.
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 20100519-21
• [Presentation] The modulation in glycosylation state of nucleoporins in cell differentiation (2010)
  • Author(s): Mizuguchi, C., Yasuhara, N., Asally, M. and Yoneda, Y
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 20100519-21
• [Presentation] Differential nuclear import is a novel regulatory mechanism for maintaining pluripotency of ES cells (2010)
  • Author(s): Yasuhara, N., Yamagishi, R., Arai, Y., Yanagida, T, Kaneko, H. and Yoneda, Y.
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 20100519-21
• [Presentation] 核-細胞質間蛋白質輸送受容体よるES細胞の分化制御機構 (2010)
  • Author(s): 安原徳子、新井由之、Rashid Mehmood、上川泰直、小川泰、柳田敏雄、米田悦啓
  • Organizer: 第27回染色体ワークショップ
  • Place of Presentation: 御殿場
  • Year and Date: 20100120-22
• [Presentation] 分化過程におけるKLFファミリータンパク質の核-細胞質間局在の解析 (2009)
  • Author(s): 金子杏美、安原徳子、米田悦啓
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] The modulation in glycosylation state of nucleoporins in cell differentiation (2009)
  • Author(s): 水口千彰、安原徳子、浅利宗弘、米田悦啓
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] Nuclear protein import machineries and cell differentiation (2009)
  • Author(s): 米田悦啓
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] Molecular dissection of the pathways underlying the nuclear import of NeuroD1, E47 and of their heterodimer (2009)
  • Author(s): Rashid Mehmood、安原徳子、米田悦啓
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-24
• [Presentation] A mobile nucleoporin, Nup98, plays an important role in a Crm1-dependent nuclear export (2009)
  • Author(s): 岡正啓、浅利宗弘、安田善也、立花太郎、米田悦啓
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-24
• [Presentation] A novel regulatory system to maintain ES cells through the nuclear-cytoplasmic transport (2009)
  • Author(s): 安原徳子、Rashid Mehmood、上川泰直、米田悦啓
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-24
• [Presentation] Nuclear protein transport and cell function(Plenary talk) (2009)
  • Author(s): Yoneda, Y.
  • Organizer: 7^<th> Annual Scientific Meeting of ARC Centre of Excellence in Biotechnology and Development
  • Place of Presentation: Kalorama, Victoria(Australia)
  • Year and Date: 20090907-08
• [Presentation] Interaction between importin alpha and Nup153 accelerates nuclear import (2009)
  • Author(s): Ogawa, Y., Miyamoto, Y., Oka, M. and Yoneda, Y.
  • Organizer: 2009 International Meeting on Nuclear Trafficking
  • Place of Presentation: Banff(Canada)
  • Year and Date: 20090824-29
• [Presentation] A novel regulatory mechanism by importin alpha and cell fate determination (2009)
  • Author(s): Yoneda, Y.
  • Organizer: 2009 International Meeting on Nuclear Trafficking
  • Place of Presentation: Banff(Canada)
  • Year and Date: 20090824-29
• [Presentation] Adaptor Aly and Co-Adaptor Thoc5Function in the Tap-p15-Mediated Nuclear Export of HSP70mRNA (2009)
  • Author(s): 片平じゅん、井上仁美、Ed Hurt、米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090602-04
• [Presentation] ペントースリン酸経路代謝酵素Transaldolaseの核内移行の解析 (2009)
  • Author(s): 福本昌宏、辻村賢二、岡正啓、米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090602-04
• [Presentation] 細胞分化におけるimportin alpha1の転写制御 (2009)
  • Author(s): 上川泰直、安原徳子、米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090602-04
• [Presentation] importin.とNup153の相互作用は核内輸送を促進する (2009)
  • Author(s): 小川泰、宮本洋一、岡正啓、米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090602-04
• [Presentation] Selective Localization of PCBP2to Cytoplamic Processing Bodies (2009)
  • Author(s): 藤村健、片平じゅん、加納ふみ、米田悦啓、村田昌之
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090602-04
• [Presentation] importin.サブタイプ間の相補性 (2009)
  • Author(s): 安東沙貴子、安原徳子、上川泰直、當間憲一、米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090602-04
• [Presentation] RanBP1欠損マウスが示す雄性不妊と成長遅滞 (2009)
  • Author(s): 永井理博、盛山哲嗣、Rashid Mehmood、徳弘圭三、伊川正人、岡部勝、田中宏光、米田悦啓
  • Organizer: 第61回日本細胞生物大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090602-04
• [Presentation] 核膜孔を介した物質輸送による核機能制御 (2009)
  • Author(s): 米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090602-04
• [Presentation] 神経細胞における核内移行情報伝達分子の検索とその分子機能解析 (2009)
  • Author(s): 福本昌宏、関元敏博、米田悦啓
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本
  • Year and Date: 20090520-22
• [Presentation] 核輸送因子による転写因子の選択的輸送機構に関する構造生物学的考察 (2009)
  • Author(s): 山岸良介、安原徳子、米田悦啓、金子寛生
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本
  • Year and Date: 20090520-22
• [Presentation] 核輸送因子Importin.の転写調節機能と精子細胞形成 (2009)
  • Author(s): 宮本洋一、安田善也、山城朋子、永井理博、米田悦啓、David A. Jans、Kate Loveland
  • Organizer: 日本分子生物学会、第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 20090511-12
• [Presentation] 核-細胞質間蛋白質輸送制御と高次生命機能 (2009)
  • Author(s): 米田悦啓
  • Organizer: 日本分子生物学会、第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 20090511-12
• [Remarks]
  • URL: http://www.anat3.med.osaka-u.ac.jp/