Analysis on the signaling network involved in the host-switching mechanism of phytoplasmas

2013 Fiscal Year Final Research Report

• Project/Area Number: 21248004
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: The University of Tokyo
• Principal Investigator: NAMBA Shigetou 東京大学, 農学生命科学研究科, 教授 (50189221)
• Project Period (FY): 2009-04-01 – 2014-03-31
• Keywords: 感染・増殖

Research Abstract

We investigated the molecular mechanism of phytoplasmal host switching to establish the basis of developing techniques to control the phytoplasma infection. Comparative analyses along with promoter analyses using phytoplasma isolate OY-M and its non-insect-transmissible mutant OY-NIM revealed that ORF3 located in the plasmid of phytoplasmas are involved in insect transmissibility. We developed phytoplasma DNA chip for the first time and performed microarray analysis to study host-specific regulation of phytoplasmal gene expressions. This analysis showed that phytoplasmas adapt to two diverse hosts, plants and insects, by dramatical alterations of their gene expression in response to the host.

Research Products

• [Journal Article] Functional characterization and gene expression profiling of superoxide dismutase from plant pathogenic phytoplasma (2012)
  • Author(s): Miura C., Sugawara K., Neriya Y., Minato N., Keima T., Himeno M., Maejima K., Komatsu K., Yamaji Y., Oshima K., Namba S
  • Journal Title: Gene Volume: 510 Pages: 107-112
• [Journal Article] Identification of three MAPKKKs forming a linear signaling pathway leading to programmed cell death in Nicotiana benthamiana (2012)
  • Author(s): Hashimoto M., Komatsu K., Maejima K., Okano Y., Shiraishi T., Ishikawa K., Takinami Y., Yamaji Y., Namba S
  • Journal Title: BMC Plant Biol Volume: 12 Pages: 103
• [Journal Article] Rapid and reliable detection of phytoplasma by loop-mediated isothermal amplification targeting a housekeeping gene (2012)
  • Author(s): Sugawara K., Himeno M., Keima T., Kitazawa Y., Maejima K., Oshima K., Namba S
  • Journal Title: J. Gen.Plant Pathol Volume: 78 Pages: 389-397
• [Journal Article] Identification and characterization of two novel genomic RNA segments of fig mosaic virus, RNA5 and RNA6 (2012)
  • Author(s): Ishikawa K., Maejima K., Komatsu K., Kitazawa Y., Hashimoto M., Takata D., Yamaji Y., Namba S
  • Journal Title: J.Gen.Virol Volume: 93 Pages: 1612-1619
• [Journal Article] Infection of capilloviruses requires subgenomic RNAs whose transcription is controlled by promoter-like sequences conserved among flexiviruses (2012)
  • Author(s): Komatsu K., Hirata H., Fukagawa T., Yamaji Y., Okano Y., Ishikawa K., Adachi T., Maejima K., Hashimoto M., Namba S
  • Journal Title: Virus Res Volume: 167 Pages: 8-15
• [Journal Article] Lectin-mediated resistance impairs plant virus infection at the cellular level (2012)
  • Author(s): Yamaji, Y., Maejima, K., Komatsu, K., Shiraish, T., Okano, Y., Himeno, M., Sugawara, K., Neriya, Y., Minato, N., Miura, C., Hashimoto, M., Namba, S
  • Journal Title: Plant Cell Volume: 24 Pages: 778-793
• [Journal Article] First report of fig mosaic virus infecting common fig (Ficus carica) in Japan (2012)
  • Author(s): Ishikawa, K., Maejima, K., Nagashima, S., Sawamura, N., Takinami, Y., Komatsu, K., Hashimoto, M., Yamaji, Y., Namba, S
  • Journal Title: J. Gen. Plant Pathol Volume: 78 Pages: 136-139
• [Journal Article] Dramatic transcriptional changes in an intracellular parasite enable host switching between plant and insect (2011)
  • Author(s): Oshima K., Ishii Y., Kakizawa S., Sugawara K., Neriya Y., Himeno M., Minato N., Miura C., Shiraishi T., Yamaji Y., Namba S
  • Journal Title: PLoS ONE Volume: 6 Pages: e23242
• [Journal Article] Cloning, expression analysis, and sequence diversity of genes encoding two different immunodominant membrane proteins in poinsettia branch-inducing phytoplasma (PoiBI) (2011)
  • Author(s): Neriya Y., Sugawara K., Maejima K., Hashimoto M., Komatsu K., Minato N., Miura C., Kakizawa S., Yamaji Y., Oshima K., Namba S
  • Journal Title: FEMS Microbiol. Lett Volume: 324 Pages: 38-47
• [Journal Article] A dual strategy for the suppression of host antiviral silencing, two distinct suppressors for viral replication and viral movement encoded by potato virus M (2011)
  • Author(s): Senshu H., Yamaji Y., Minato N., Shiraishi T., Maejima K., Hashimoto M., Miura C., Neriya Y., Namba S
  • Journal Title: J. Virol Volume: 85 Pages: 10269-10278
• [Journal Article] Unique morphological changes in plant pathogenic phytoplasma-infected petunia flowers are related to transcriptional regulation of floral homeotic genes in an organ-specific manner (2011)
  • Author(s): Himeno, M., Neriya, Y., Minato, N., Miura, C., Sugawara, K., Ishii, Y., Yamaji, Y., Kakizawa, S., Oshima, K., Namba, S
  • Journal Title: Plant J Volume: 67 Pages: 971-979
• [Journal Article] New detection systems of bacteria using highly selective media designed by SMART : Selective Medium-Design Algorithm Restricted by Two constraints (2011)
  • Author(s): Kawanishi, T., Shiraishi, T., Okano, Y., Sugawara, K., Hashimoto, M., Maejima, K., Komatsu, K., Kakizawa, S., Yamaji, Y., Hamamoto, H., Oshima, K., Namba, S
  • Journal Title: PLoS ONE Volume: 6 Pages: e16512
• [Journal Article] First report of Helleborus net necrosis virus isolated from hellebores with black death syndrome in Japan (2011)
  • Author(s): Shiraishi T., Hoshi H., Eimori K., Kawanishi T., Komatsu K., Hashimoto M., Maejima K., Yamaji Y., Namba S
  • Journal Title: J. Gen. Plant Pathol Volume: 77 Pages: 269-272
• [Journal Article] A necrosis-inducing elicitor domain encoded by both symptomatic and asymptomatic Plantago asiatica mosaic virus isolates, whose expression is modulated by virus replication (2011)
  • Author(s): Komatsu, K., Hashimoto, M., Maejima, K., Shiraishi, T., Neriya, Y., Miura, C., Minato, N., Okano, Y., Sugawara, K., Yamaji, Y., Namba, S
  • Journal Title: Mol. Plant-Microbe Interact Volume: 24 Pages: 408-420
• [Journal Article] Molecular epidemiology of plum pox virus in Japan (2011)
  • Author(s): Maejima, K., Himeno, M., Komatsu, K., Takinami, Y., Hashimoto, M., Takahashi, S., Yamaji, Y., Oshima, K., Namba, S
  • Journal Title: Phytopathology Volume: 101 Pages: 567-574
• [Journal Article] First report of Choanephora rot of ice plant (Mesembryanthemum crystallinum) caused by Choanephora cucurbitarum in Japan (2010)
  • Author(s): Kagiwada S., Kayano Y., Hoshi H., Kawanishi T., Oshima K., Hamamoto H., Horie H., Namba S
  • Journal Title: J. Gen. Plant Pathol Volume: 76 Pages: 345-347
• [Journal Article] Inhibitory effect on the tobacco mosaic virus infection by a plant RING finger protein (2010)
  • Author(s): Yamaji, Y., Hamada, K., Yoshinuma, T., Sakurai, K., Yoshii, A., Shimizu, T., Hashimoto, M., Suzuki, M., Namba, S., Hibi, T
  • Journal Title: Virus Res Volume: 152 Pages: 50-57
• [Journal Article] Significantly low level of small RNA accumulation derived from an encapsidated mycovirus with dsRNA genome (2010)
  • Author(s): Himeno, M., Maejima, K., Komatsu, K,. Ozeki, J., Hashimoto, M., Kagiwada, S., Yamaji, Y., Namba, S
  • Journal Title: Virology Volume: 396 Pages: 69-75
• [Journal Article] Viral-induced systemic necrosis in plants involves both programmed cell death and the inhibition of viral multiplication, which are regulated by independent pathways (2010)
  • Author(s): Komatsu, K., Hashimoto, M., Ozeki, J., Yamaji, Y., Maejima, K., Senshu, H., Himeno, M., Okano, Y., Kagiwada, S., Namba, S
  • Journal Title: Mol.Plant-Microbe Interact Volume: 23 Pages: 283-293
• [Journal Article] Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection (2010)
  • Author(s): Yamaji, Y., Sakurai, K., Hamada, K., Komatsu, K., Ozeki, J., Yoshida, A., Yoshii, A., Shimizu, T., Namba, S., Hibi, T
  • Journal Title: Arch.Virol Volume: 155 Pages: 263-268
• [Journal Article] Genetic heterogeneity found in the replicase gene of poinsettia mosaic virus isolates (2010)
  • Author(s): Okano, Y., Maejima, K., Shiraishi, T., Hashimoto, M., Senshu, H., Ozeki, J., Takahashi, S., Komatsu, K., Yamaji, Y., Namba, S
  • Journal Title: Arch. Virol Volume: 155 Pages: 1367-1370
• [Journal Article] Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication (2010)
  • Author(s): Hirata, H., Yamaji, Y., Komatsu, K., Kagiwada, S., Oshima, K., Okano, Y., Takahashi, S., Ugaki, M., Namba, S
  • Journal Title: Virus Res Volume: 152 Pages: 1-9
• [Journal Article] First report of plum pox virus infecting Japanese apricot (Prunus mume Sieb. et Zucc.) in Japan (2010)
  • Author(s): Maejima, K., Hoshi, H., Hashimoto, M., Himeno, M., Kawanishi, T., Komatsu, K., Yamaji, Y., Hamamoto, H., Namba, S
  • Journal Title: J. Gen. Plant Pathol Volume: 76 Pages: 229-231
• [Journal Article] Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBp1) from various potexviruses during infection (2009)
  • Author(s): Senshu, H., Ozeki, J., Komatsu, K., Hashimoto, M., Hatada, K., Aoyama, M., Kagiwada, S., Yamaji, Y., Namba, S
  • Journal Title: J. Gen. Virol Volume: 90 Pages: 1014-1024
• [Journal Article] Cloning of immunodominant membrane protein genes of phytoplasmas and their in planta expression (2009)
  • Author(s): Kakizawa, S., Oshima, K., Ishii, Y., Hoshi, A., Maejima, K., Jung, H.Y., Yamaji, Y., Namba, S
  • Journal Title: FEMS Microbiol. Lett Volume: 293 Pages: 92-101
• [Journal Article] A unique virulence factor for proliferation and dwarfism in plants identified from a phytopathogenic bacterium (2009)
  • Author(s): Hoshi, A., Oshima, K., Kakizawa, S., Ishii, Y., Ozeki, J., Hashimoto, M., Komatsu, K., Kagiwada, S., Yamaji, Y., Namba, S
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A Volume: 106 Pages: 6416-6421
• [Journal Article] In the non-insect-transmissible line of Onion Yellows phytoplasma (OY-NIM), the plasmid encoded, transmembrane protein ORF3 lacks the major promoter region (2009)
  • Author(s): Ishii, Y., Kakizawa, S., Hoshi, A., Maejima, K., Kagiwada, S., Yamaji, Y., Oshima, K., Namba, S
  • Journal Title: Microbiology-(UK) Volume: 155 Pages: 2058-2067
• [Journal Article] The N-terminal region of the Plantago asiatica mosaic virus coat protein is required for cell-to-cell movement, but is dispensable for virion assembly (2009)
  • Author(s): Ozeki, J., Hashimoto, M., Komatsu, K., Maejima, K., Himeno, M., Senshu, H., Kawanishi, T., Kagiwada, S., Yamaji, Y., Namba, S
  • Journal Title: Mol. Plant-Microbe Interact Volume: 22 Pages: 677-685
• [Journal Article] Identification of a novel tobacco DnaJ-like protein that interacts with the movement protein of tobacco mosaic virus (2009)
  • Author(s): Shimizu, T., Yoshii, A., Sakurai, K., Hamada, K., Yamaji, Y., Suzuki, M., Namba, S., Hibi, T
  • Journal Title: Arch. Virol Volume: 154 Pages: 959-967
• [Journal Article] Process of reductive evolution during 10 years in plasmids of a non-insect-transmissible phytoplasma (2009)
  • Author(s): Ishii, Y., Oshima, K., Kakizawa, S., Hoshi, A., Maejima, K., Kagiwada, S., Yamaji, Y., Namba, S
  • Journal Title: Gene Volume: 446 Pages: 51-57
• [Journal Article] Complete nucleotide sequence and genome organization of butterbur mosaic virus (2009)
  • Author(s): Hashimoto, M., Komatsu, K., Maejima, K., Yamaji, Y., Okano, Y., Shiraishi, T., Takahashi, S., Kagiwada, S., Namba, S
  • Journal Title: Arch. Virol Volume: 154 Pages: 1955-1958
• [Presentation] 葉化症状を示すアジサイより検出されたCandidatus Phytoplasma asteris HP系統の分子遺伝学的解析 (2013)
  • Author(s): 桂馬拓也・高橋厚・滝波祐輔・岡野夕香里・前島健作・大島研郎・難波成任
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜大学
  • Year and Date: 20130327-29
• [Presentation] 植物病原細菌ファイトプラズマのペプチド性エフェクターTENGUの機能解析 (2012)
  • Author(s): 菅原杏子・湊菜未・小松健・大島研郎・難波成任
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 20120915-17
• [Presentation] 葉化症状を引き起こすファイトプラズマのエフェクターについて (2012)
  • Author(s): 前島健作・岩井涼・藤田尚子・姫野未紗子・山次康幸・大島研郎・難波成任
  • Organizer: 平成24年度日本植物病理学会関東部会
  • Place of Presentation: 法政大学
  • Year and Date: 20120913-14
• [Presentation] Molecular biological studies on phytoplasmal pathogenicity (2012)
  • Author(s): Namba S
  • Organizer: XV International congress of molecular plant-microbe interactions
  • Place of Presentation: 京都国際会館
  • Year and Date: 20120729-0802
• [Presentation] Detection of two strains of 'Candidatus phytoplasma asteris' from peach and apricot using loopmediated isothermal amplification (2012)
  • Author(s): Miura C., Keima T.,Watanabe T., Nijo T., Maejima K., Oshima K. and Namba S
  • Organizer: 22nd 'International conference on virus and other graft transmissible diseases of fruit crops' ICVF
  • Place of Presentation: Rome, Italy
  • Year and Date: 20120603-08
• [Presentation] ファイトプラズマ感染が花の形態形成遺伝子の発現へ与える影響 (2012)
  • Author(s): 姫野未紗子・湊菜未・煉谷裕太朗・三浦千 裕・石川一也・桂馬拓也・前島健作・大島研郎・難波成任
  • Organizer: 日本マイコプラズマ学会第39回学術集会
  • Place of Presentation: いわて県民情報交流センター
  • Year and Date: 20120524-25
• [Presentation] ファイトプラズマのポストゲノム生物学-宿主スイッチングにおける網羅的遺伝子解析- (2012)
  • Author(s): 大島研郎・三浦千裕・湊菜未・煉谷裕太朗・石川一也・北沢優悟・二條貴通・渡邊翼・前島健作・難波成任
  • Organizer: 日本マイコプラズマ学会第39回学術集会
  • Place of Presentation: いわて県民情報交流センター
  • Year and Date: 20120524-25
• [Presentation] ファイトプラズマ感染ペチュニアにおける花のホメオティック遺伝子の発現変動解析 (2012)
  • Author(s): 姫野未紗子・菅原杏子・岩井涼・煉谷裕太朗・小松健・大島研郎・難波成任
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20120328-30
• [Presentation] ファイトプラズマ感染ペチュニアにおける花芽分裂組織遺伝子の発現変動 (2012)
  • Author(s): 岩井涼・姫野未紗子・菅原杏子・煉谷裕太朗・小松健・大島研郎・難波成任
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20120328-30
• [Presentation] 機械刺激受容チャネル阻害によるファイトプラズマ増殖抑制の可能性 (2012)
  • Author(s): 湊菜未・三浦千裕・桂馬拓也・北沢優悟・前島健作・大島研郎・難波成任
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20120328-30
• [Presentation] LAMP法を用いたファイトプラズマ病診断キットの開発 (2012)
  • Author(s): 菅原杏子・滝波祐輔・前島健作・足立達司・白石拓也・橋本将典・難波成任
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20120328-30
• [Presentation] Poinsettia branch-inducing phytoplasmaPoiBI の主要抗原膜タンパク質の発現解析 (2012)
  • Author(s): 煉谷裕太朗・岩井涼・滝波祐輔・桂馬拓也・前島健作・大島研郎・難波成任
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20120328-30
• [Presentation] 植物-昆虫ホストスイッチングに伴うファイトプラズマ遺伝子の網羅的発現変動解析 (2012)
  • Author(s): 三浦千裕・湊菜未・北沢優悟・前島健作・大島研郎・難波成任
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20120328-30
• [Presentation] ファイトプラズマの網羅的遺伝子発現解析系の確立 (2012)
  • Author(s): 大島研郎・三浦千裕・湊菜未・北沢優悟・姫野未紗子・前島健作・難波成任
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20120328-30
• [Presentation] New Developments in Molecular Biological Research on Plant-Pathogenic Mollicutes : ATiny Phytoplasma with a Reduced Genome tricks a Giant Plant Industry (2011)
  • Author(s): Namba S
  • Organizer: The 5th Meeting of the Asian Organization for Mycoplasmology
  • Place of Presentation: 長崎県医師会館
  • Year and Date: 20111019-21
  • Invited
• [Presentation] 葉化症状を示すアジサイより初めて検出されたファイトプラズマ種とその検出キットの開発について (2011)
  • Author(s): 滝波祐輔・高橋厚・石川一也・足立達司・三浦千裕・煉谷裕太朗・前島健作・難波成任
  • Organizer: 平成23年度日本植物病理学会関東部会
  • Place of Presentation: 文部科学省研究交流センター
  • Year and Date: 20110915-16
• [Presentation] Expression changes of floral meristem identity genes in phytoplasma-infected petunia (2011)
  • Author(s): Sugawara K., Himeno M., Oshima K., Namba S
  • Organizer: The second meeting of the International PhytoplasmologistWorking Group
  • Place of Presentation: Neustadt an derWeinstrasse, Germany
  • Year and Date: 20110912-15
• [Presentation] Molecular analysis of genes encoding two different immunodominant membrane proteins in poinsettia branch-inducing phytoplasma (PoiBI) (2011)
  • Author(s): Neriya Y., Maejima K., Yamaji Y., Oshima K., Namba S
  • Organizer: The second meeting of the International PhytoplasmologistWorking Group
  • Place of Presentation: Neustadt an der Weinstrasse, Germany
  • Year and Date: 20110912-15
• [Presentation] Molecular mechamisms underlying the pathogenicity of phytoplasma (2011)
  • Author(s): Oshima K., Sugawara K., Himeno M., Adachi T., Ishikawa K., Takinami Y., Namba S
  • Organizer: XIII, International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 20110906-10
  • Invited
• [Presentation] New Developments in Molecular Biological Research on Plant-Pathogenic Mollicutes : Atiny Phytoplasma with a Reduced Genome tricks a Giant Plant Industry (2010)
  • Author(s): Namba, S
  • Organizer: 18th International Congress of the International Organization for Mycoplasmology
  • Place of Presentation: Chianciano Terme, Italy
  • Year and Date: 20100711-16
• [Book] 植物病理学 (2010)
  • Author(s): 真山滋志, 難波成任
  • Total Pages: 328
  • Publisher: 文永堂出版
• [Patent(Industrial Property Rights)] 植物の分枝形成促進, 矮化, 不稔化, および花芽複数形成促進方法 (2010)
  • Inventor(s): 難波成任, 大島研郎
  • Industrial Property Rights Holder: 国立大学法人 東京大学
  • Industrial Property Number: 特許公開2010-43024
  • Acquisition Date: 2010-02-25