Identification and functional analysis of the genes controlling the relationship between lepidopteran insects and their host plants

2011 Fiscal Year Final Research Report

• Project/Area Number: 21248006
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: The University of Tokyo
• Principal Investigator: SHIMADA Toru 東京大学, 大学院・農学生命科学研究科, 教授 (20202111)
• Co-Investigator(Kenkyū-buntansha): DAIMON Takaaki 農業生物資源研究所, 昆虫制御研究ユニット, 主任研究員 (70451846)
• Project Period (FY): 2009 – 2011
• Keywords: 植食性昆虫 / カイコ / クワ / 糖類似アルカロイド

Research Abstract

The lepidopteran insects have specific relationships with their host plants. The mulberry contains toxic sugar-mimic alkaloids s, and the insects fed on mulberry must overcome their toxicity. We compared the function of the midut sucrases, which may play a crucial role in the tolerance to the alkaloids, between mulberry feeders such as Bombyx mori and Rondotia menciana and closely-related non-mulberry feeders such as Trilocha varians. As the result, we found that mRNAs for alkaloid-resistant sucrases are more highly expressed in mulberry feeders than in non-mulberry ones. We also found a gene encoding an enzyme that plays an important role in the metabolism of quercetin, a component in mulberry.

Research Products

• [Journal Article] Female sex pheromone and male behavioral responses of the bombycid moth Trilocha varians : comparison with those of the domes-ticated silkmoth Bombyx mori. (2012)
  • Author(s): Surivilai, D. Lirdwitayaprasit, T. and Fukuyo, Y.
  • Journal Title: Naturwissenschaften Volume: Vol.99、No.3 Pages: 207-215
  • Peer Reviewed
• [Journal Article] Molecular phylogeny, laboratory rearing, and karyotype of the bombycid moth, Trilocha varians(Lepidoptera : Bombycidae : Bombycinae) (2012)
  • Author(s): Takaaki Daimon, Masaya Yago, Yu-Feng Hsu, Tsuguru Fujii, Yumiko Nakajima, Ryuhei Kokusho, Hiroaki Abe, Susumu Katsuma, and Toru Shimada
  • Journal Title: Journal of Insect Science Volume: Vol.12、No.49
  • URL: http://insectscience.org/12.49/
  • Peer Reviewed
• [Journal Article] Interspecies linkage analy-sis of mo, a Bombyx mori locus associated with mosaicism and gynandromorphism. (2011)
  • Author(s): Tsuguru Fujii, Hiroaki Abe, Kimiko Yamamoto, Susumu Katsuma, and Toru Shi-mada
  • Journal Title: Genetica Volume: Vol.139、No.10 Pages: 1323-1329
  • Peer Reviewed
• [Journal Article] Sex-linked transcription factor involved in a shift of sex pheromone preference in the silkmoth, Bombyx mori (2011)
  • Author(s): Tsuguru Fujii, Takeshi Fujii, Shigehiro Namiki, Hiroaki Abe, Takeshi Sakurai, Akio Ohnuma, Ryohei Kanzaki, Susumu Katsuma, Yukio Ishikawa, and Toru Shimada
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: Vol.108、No.44 Pages: 18038-18043
  • Peer Reviewed
• [Journal Article] Molecular defect of isovaleryl-CoA dehydrogenate in the skunk mutant of silkworm, Bombyx mori (2010)
  • Author(s): Kei Urano, Takaaki Daimon, Yutaka Banno, Kazuei Mita, Tohru Terada, Kentaro Shimizu, Susumu Katsuma, and Toru Shimada
  • Journal Title: FEBS Journal Volume: Vol.277、No.21 Pages: 4452-4463
  • Peer Reviewed
• [Journal Article] The silkworm Green b locus encodes a quercetin 5-0-glucosyltransferase that produces green cocoons with UV-shielding properties (2010)
  • Author(s): Takaaki Daimon, Chikara Hirayama, Masatoshi Kanai, Yoshinao Ruike, Yan Meng, Eiichi Kosegawa, Masatoshi Nakamura, Gozoh Tsujimoto, Susumu Katsuma, and Toru Shimada
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: Vol.107、No.25 Pages: 11471-11476
  • Peer Reviewed
• [Presentation] カイコガ科のクワへの適応におけるクワ乳液成分に対する抵抗性獲得の重要性 (2012)
  • Author(s): 加藤裕子・木内隆史・王華兵・大門高明・勝間進・嶋田透
  • Organizer: 日本蚕糸学会第82回大会
  • Place of Presentation: 九州大学箱崎キャンパス文系地区(福岡県福岡市東区箱崎)
  • Year and Date: 20120318-19
• [Presentation] 第11染色体を部分的に欠失したカイコの食性異常 (2012)
  • Author(s): 藤井告・久保真紀・阿部広明・大沼昭夫・桑崎誠剛・山本公子・川本宗孝・勝間進・嶋田透
  • Organizer: 日本蚕糸学会第82回大会
  • Place of Presentation: 九州大学箱崎キャンパス文系地区(福岡県福岡市東区箱崎)
  • Year and Date: 20120318-19
• [Presentation] Transcriptional regulation ofβ-fructofuranosidase gene in the silkworm, Bombyx mori (2012)
  • Author(s): 王華兵・木内隆史・國生龍平・大門高明・勝間進・嶋田透
  • Organizer: 日本蚕糸学会第82回大会
  • Place of Presentation: 九州大学箱崎キャンパス文系地区(福岡県福岡市東区箱崎)
  • Year and Date: 20120318-19
• [Presentation] カイコとその近縁種における寄主植物選択機構の進化 (2012)
  • Author(s): 木内隆史・王華兵・加藤裕子・藤井告・大門高明・門田幸二・鈴木穣・菅野純夫・勝間進・嶋田透
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 近畿大学農学部・奈良キャンパス(奈良県奈良市)
  • Year and Date: 2012-03-28
• [Presentation] カイコの変異体を用いた嗅覚・味覚の分子遺伝学的研究 (2012)
  • Author(s): 嶋田透、藤井告、藤井毅、並木重宏、阿部広明、櫻井健志、大沼昭夫、神崎亮平、勝間進、石川幸男
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 奈良県新公会堂(奈良県奈良市)
  • Year and Date: 2012-03-27
• [Presentation] Genetic and genomic studies on the food habit of the silkworm (2012)
  • Author(s): Toru Shimada, Takaaki Daimon, Tsuguru Fujii, Yan Meng, Huabing Wang, Hiroaki Abe, Akio Onuma, Takashi Kiuchi, and Susumu Katsuma
  • Organizer: IITG-DBT Biotech Hub Invited Lecture Series
  • Place of Presentation: Seminar Hall, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati Assam, India
  • Year and Date: 2012-02-28
• [Presentation] Genetic and genomic studies on the food habits of the silkworm (2012)
  • Author(s): Toru Shimada, Takaaki Daimon, Tsuguru Fujii, Yan Meng, Huabing Wang, Hiroaki Abe, Akio Onuma, Takashi Kiuchi, and Susumu Katsuma
  • Organizer: Special Lecture for the Silver Jubilee of the Department of Biotechnology(DBT), Ministry of Science & Technology, Indian Government
  • Place of Presentation: CDFD Lecture Hall, 2nd Floor, Lab Building, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad, India
  • Year and Date: 2012-02-24
• [Presentation] Genomic studies on the relationship between the silkworm and its food plant, mulberry (2012)
  • Author(s): Toru Shimada, Takaaki Daimon, Tsuguru Fujii, Yan Meng, Huabing Wang, Hiroaki Abe, Akio Onuma, Takashi Kiuchi, and Susumu Katsuma
  • Organizer: Genome Research : Current Challenges and Future Directions
  • Place of Presentation: KOKUYO HALL(東京都港区港南)
  • Year and Date: 2012-01-21
• [Presentation] 鱗翅目昆虫におけるβ-フルクトフラノシダーゼ遺伝子の転写調節 (2011)
  • Author(s): 王華兵・木内隆史・國生龍平・大門高明・勝間進・嶋田透
  • Organizer: 平成23年度日本蚕糸学会第66回東北支部・第62回関東支部・第77回関西支部・第67回九州支部合同大会-蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 岩手大学農学部(岩手県盛岡市)
  • Year and Date: 20111104-06
• [Presentation] 各種クワ科植物に対するカイコとイチジクカサンの摂食性 (2011)
  • Author(s): 加藤裕子・王華兵・木内隆史・大門高明・矢後勝也・中島裕美子・前川秀彰・Yu-Feng Hsu・藤井告・勝間進・嶋田透
  • Organizer: 平成23年度日本蚕糸学会第66回東北支部・第62回関東支部・第77回関西支部・第67回九州支部合同大会-蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 岩手大学農学部(岩手県盛岡市)
  • Year and Date: 20111104-06
• [Presentation] Genetic studies on the relationship between the silkworm, Bombyx mori, and its host plant, mulberry (2011)
  • Author(s): Toru Shimada, Takaaki Daimon, Tsuguru Fujii, Yan Meng, Yuko Kato, Huabing Wang, Hiroaki Abe, Akio Onuma, Takashi Kiuchi, and Susumu Katsuma
  • Organizer: The 2nd International Symposium on Bombyx mori Functional Genomics and Modern Silk Road
  • Place of Presentation: 中国・西南大学・蚕学与系統生物学研究所
  • Year and Date: 20111022-23
• [Presentation] ゲノム情報を用いたカイコの食性進化メカニズムの解明 (2011)
  • Author(s): 嶋田透・孟艶・吉永武史・國生龍平・王華兵・加藤裕子・木内隆史・藤井告・勝間進・大門高明
  • Organizer: 第84回日本生化学会大会シンポジウム「ゲノム時代の生化学」
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] カイコガ科におけるβ-フルクトフラノシダーゼ遺伝子の進化 (2010)
  • Author(s): 國生龍平・大門高明・平山力・矢後勝也・Yu-Feng Hsu・中島裕美子・勝間進・嶋田透
  • Organizer: 日本蚕糸学会第80回記念大会
  • Place of Presentation: 信州大学繊維学部
  • Year and Date: 20100403-04
• [Presentation] カイコの笹繭b(Gb)の原因遺伝子の同定 (2010)
  • Author(s): 大門高明・平山力・勝間進・嶋田透
  • Organizer: 日本蚕糸学会第80回記念大会
  • Place of Presentation: 信州大学繊維学部
  • Year and Date: 20100403-04
• [Presentation] Enzymatic adaptation of mulberry specialists to toxic sugar-mimic alkaloids in mulberry latex (2009)
  • Author(s): Takaaki Daimon, Yan Meng, Susumu Katsuma, and Toru Shimad
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20091209-12
• [Presentation] カイコはなぜクワを食うかーゲノムからのヒント (2009)
  • Author(s): 嶋田透
  • Organizer: 日本蚕糸学会関東支部第60回学術講演会公開シンポジウム「カイコゲノム情報の今後の展開」
  • Place of Presentation: 宇都宮大学大学会館多目的ホール
  • Year and Date: 20091106-07
• [Presentation] カイコガ科におけるβ-フルクトフラノシダーゼ遺伝子の進化 (2009)
  • Author(s): 國生龍平・大門高明・矢後勝也・Yu-Feng Hsu・中島裕美子・勝間進・嶋田透
  • Organizer: 日本蚕糸学会関東支部第60回学術講演会
  • Place of Presentation: 宇都宮大学大学会館多目的ホール
  • Year and Date: 20091106-07
• [Presentation] カイコ食性異常遺伝子Npのポジショナルクローニングの試み (2009)
  • Author(s): 久保真紀、藤井告、大沼昭夫、勝間進、嶋田透
  • Organizer: 日本蚕糸学会関東支部第60回学術講演会
  • Place of Presentation: 宇都宮大学大学会館多目的ホール
  • Year and Date: 20091106-07
• [Presentation] How did Bombyx adapt to mulberry?-A hypothesis in comparative genomics (2009)
  • Author(s): Toru Shimada, Takaaki Daimon, Tsuguru Fujii, Yan Meng, Takeshi Yoshinaga1, Akio Ohnuma, Chikara Hirayama, Susumu Katsuma, Hiroaki Abe, and Kazuei Mita
  • Organizer: Inter-national Symposium on Bombyx mori Functional Genomics and Modern Silk Road
  • Place of Presentation: 海宇温泉大酒店、中国・重慶市北碚区双元大道198号
  • Year and Date: 20091021-23
• [Presentation] Genetic mechanisms controlling the food habit of Bombyx mori, a monophagous insect depending on mulberry (2009)
  • Author(s): Toru Shimada, Takaaki Daimon, Tsuguru Fujii, Yan Meng, Takeshi Yoshinaga, Akio Ohnuma, Chikara Hirayama, Susumu Katsuma, Hiroaki Abe, and Kazuei Mita
  • Organizer: THE 6TH Ais-Pacific Congress of Entomology(APCE 2009)-Symposium 6(Genetics, Genomics, Transgenics & Evolutionary Biology)
  • Place of Presentation: 九華山庄(中国・北京市昌平区小湯山)
  • Year and Date: 20091018-22
• [Presentation] カイコのspli変異体に認められる食性と性フェロモン応答性の異常 (2009)
  • Author(s): 藤井告・藤井毅・桑崎誠剛・山本公子・阿部広明・大沼昭夫・勝間進・三田和英・嶋田透
  • Organizer: 昆虫ポストゲノム研究会
  • Place of Presentation: 北海道大学遠遊学舎/農学部総合研究棟1F
  • Year and Date: 20090909-11
• [Presentation] Enzymatic adaptation of mulberry specialists to toxic sugar-mimic alkaloids in mulberry latex (2009)
  • Author(s): Takaaki Daimon, Yan Meng, Takeshi Yoshinaga, Susumu Katsuma, and Toru Shimada
  • Organizer: The 8th International Workshop on Molecular Biology and Genetics o the Lepidoptera
  • Place of Presentation: Orthodox Academy in Kolympari, Crete, Greece
  • Year and Date: 20090823-29
• [Presentation] acj6 mutants of the silkworm Bombyx mori cannot discriminate mulberry from beet leaves (2009)
  • Author(s): Tsuguru Fujii, Takeshi Fujii, Hiroaki Abe, Akio Ohnuma, Susumu Katsuma, Toru Shimada
  • Organizer: The 8th International Workshop on Molecular Biology and Genetics o the Lepidoptera
  • Place of Presentation: Orthodox Academy in Kolympari, Crete, Greece
  • Year and Date: 20090823-29