Molecular cloning and characterization of genes responsible for the diapause-initiation and the diapause-termination induced by cold in Bombyx mori eggs

2011 Fiscal Year Final Research Report

• Project/Area Number: 21248007
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Nagoya University
• Principal Investigator: YAGINUMA Toshinobu 名古屋大学, 生命農学研究科, 教授 (60135332)
• Co-Investigator(Kenkyū-buntansha): NIIMI Teruyuki 名古屋大学, 大学院・生命農学研究科, 准教授 (00293712) ; IKEDA Motoko 名古屋大学, 大学院・生命農学研究科, 准教授 (20262892) ; KADONO Keiko (独)農業生物資源研究所, 主任研究員 (40355722) ; SHIOMI Kunihiro 信州大学, 繊維学部, 准教授 (70324241)
• Project Period (FY): 2009 – 2011
• Keywords: カイコ / 胚休眠 / 休眠開始遺伝子 / 休眠覚醒 / 低温誘導性遺伝子 / 低温シグナル受容・伝達

Research Abstract

We cloned pnd-1 and-2 genes that have been known as genes initiating Bombyx mori embryonic diapause, using both procedures of positional cloning and RNA interference. We also cloned a strong candidate gene connected the diapause hormone signal to the expression of pnd genes, using DNA microarray analysis. We isolated a gene for heat shock transcription factor(HSF) as a factor transducing the 5℃signal. HSF was shown to bind to heat shock elements at 5'-upstreams of heat shock protein 70a and Samui genes to confer their transcription activities.

Research Products

• [Journal Article] Trehalase-2 protein contributes to trehalase activity enhanced by diapause hormone in developing ovaries of the silkworm (2011)
  • Author(s): Kamei, Y., Hasegawa, Y., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: Bombyx mori. J. Insect Physiol Volume: 57(5) Pages: 608-613
  • Peer Reviewed
• [Journal Article] Cloning of cDNAs encoding sorbitol dehydrogenase-2a and b, enzymatic characterization and up-regulated expression of the genes in Bombyx mori diapause eggs exposed to 5℃ (2011)
  • Author(s): Rubio, R. O., Suzuki, A., Mitsumasu, K., Homma, T., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: Insect Biochem. Mol. Biol Volume: 41(6) Pages: 378-387
  • Peer Reviewed
• [Journal Article] Heat shock factor binds to heat shock elements at 5'-upstreams of heat shock protein 70a and Samui genes to confer their transcription activities in Bombyx mori diapause eggs exposed to 5℃ (2011)
  • Author(s): Kihara, F., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: Insect Biochem. Mol. Biol Volume: 41(11) Pages: 843-851
  • Peer Reviewed
• [Journal Article] Expression of heat shock protein 70a mRNA in Bombyx mori diapause eggs (2010)
  • Author(s): Moribe, Y., Oka, K., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: J. Insect Physiol Volume: 56 Pages: 1246-1252
  • Peer Reviewed
• [Journal Article] Disappearance of chorion proteins in Bombyx mori eggs treated with HCl solution to prevent diapause (2010)
  • Author(s): Tsurumaru, S., Kawamori, A., Mitsumasu, K., Niimi, T., Imai, K., Yamashita, O. and Yaginuma, T
  • Journal Title: J. Insect Physiol Volume: 56 Pages: 1721-1727
  • Peer Reviewed
• [Journal Article] Comparative pharmacology of two D1-like dopamine receptors cloned from the silkworm Bombyx mori (2009)
  • Author(s): Ohta, H., Tsuchihara, K., Mitsumasu, K., Yaginuma, T., Ozoe, Y. and Asaoka, K
  • Journal Title: Insect Biochem. Mol. Biol Volume: 39 Pages: 342-347
  • Peer Reviewed
• [Journal Article] Novel gene encoding precursor protein consisting of possible several neuropeptides expressed in brain and frontal ganglion of the silkworm (2009)
  • Author(s): Mitsumasu, K., Tanaka, Y., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: Bombyx mori. Peptides Volume: 30 Pages: 1233-1240
  • Peer Reviewed
• [Journal Article] Glycerol kinase activity and glycerol kinase-3 gene are up-regulated by acclimation to 5℃in diapause eggs of the silkworm (2009)
  • Author(s): Kihara, F., Itoh, K., Iwasaka, M., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: Bombyx mori. Insect Biochem. Mol. Biol Volume: 39 Pages: 763-769
  • Peer Reviewed
• [Presentation] Bombyx mori embryonic diapause : transducers of diapause hormone signal for diapause initiation and 5℃signal for diapause termination (2011)
  • Author(s): Yaginuma, T., Suzuki, A., Kihara, F., Kamei, Y., Niimi, T., Yamashita, O
  • Organizer: The Sixth International Symposium on Molecular Insect Science
  • Place of Presentation: アムステルダム
  • Year and Date: 20111002-05
• [Presentation] 温暖化と昆虫-特に休眠の観点から- (2010)
  • Author(s): 柳沼利信
  • Organizer: 名古屋大学大学院生命農学研究科附属フィールド科学教育研究センター東郷フィールド講演会
  • Place of Presentation: 東郷町
  • Year and Date: 2010-06-26
• [Presentation] 形質と環境-カイコ胚休眠の観点から- (2009)
  • Author(s): 柳沼利信
  • Organizer: 日大理学部自然科学研究所シンポジウム「動物の不思議を科学する」
  • Place of Presentation: 日本大学
  • Year and Date: 2009-06-20
• [Book] 休眠ホルモンによるカイコガ胚休眠の調節脱皮・変態の生物学-昆虫と甲殻類のホルモン作用の謎を追う-(園部治之・長澤寛道編) (2011)
  • Author(s): 柳沼利信,新美輝幸,塩見邦博
  • Total Pages: 263-285
  • Publisher: 東海大学出版会
• [Book] 低温誘導性遺伝子Samui.昆虫の低温耐性-その仕組みと調べ方-(積木久明・田中一裕・後藤三千代編) (2010)
  • Author(s): 柳沼利信
  • Total Pages: 225-233
  • Publisher: 岡山大学出版会
• [Book] カイコ胚休眠の生化学・分子生物学虫たちが語る生物学の未来(衣笠会編) (2009)
  • Author(s): 柳沼利信
  • Total Pages: 146-153
  • Publisher: (財団法人)衣笠会
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/.yousan/