2011 Fiscal Year Final Research Report
Molecular cloning and characterization of genes responsible for the diapause-initiation and the diapause-termination induced by cold in Bombyx mori eggs
| Project/Area Number |
21248007
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NIIMI Teruyuki 名古屋大学, 大学院・生命農学研究科, 准教授 (00293712)
IKEDA Motoko 名古屋大学, 大学院・生命農学研究科, 准教授 (20262892)
KADONO Keiko (独)農業生物資源研究所, 主任研究員 (40355722)
SHIOMI Kunihiro 信州大学, 繊維学部, 准教授 (70324241)
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| Project Period (FY) |
2009 – 2011
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| Keywords | カイコ / 胚休眠 / 休眠開始遺伝子 / 休眠覚醒 / 低温誘導性遺伝子 / 低温シグナル受容・伝達 |
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| Research Abstract |
We cloned pnd-1 and-2 genes that have been known as genes initiating Bombyx mori embryonic diapause, using both procedures of positional cloning and RNA interference. We also cloned a strong candidate gene connected the diapause hormone signal to the expression of pnd genes, using DNA microarray analysis. We isolated a gene for heat shock transcription factor(HSF) as a factor transducing the 5℃signal. HSF was shown to bind to heat shock elements at 5'-upstreams of heat shock protein 70a and Samui genes to confer their transcription activities.
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[Journal Article] Cloning of cDNAs encoding sorbitol dehydrogenase-2a and b, enzymatic characterization and up-regulated expression of the genes in Bombyx mori diapause eggs exposed to 5℃2011
Author(s)
Rubio, R. O., Suzuki, A., Mitsumasu, K., Homma, T., Niimi, T., Yamashita, O. and Yaginuma, T
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Journal Title
Insect Biochem. Mol. Biol
Volume: 41(6)
Pages: 378-387
Peer Reviewed
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[Journal Article] Disappearance of chorion proteins in Bombyx mori eggs treated with HCl solution to prevent diapause2010
Author(s)
Tsurumaru, S., Kawamori, A., Mitsumasu, K., Niimi, T., Imai, K., Yamashita, O. and Yaginuma, T
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Journal Title
J. Insect Physiol
Volume: 56
Pages: 1721-1727
Peer Reviewed
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[Journal Article] Comparative pharmacology of two D1-like dopamine receptors cloned from the silkworm Bombyx mori2009
Author(s)
Ohta, H., Tsuchihara, K., Mitsumasu, K., Yaginuma, T., Ozoe, Y. and Asaoka, K
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Journal Title
Insect Biochem. Mol. Biol
Volume: 39
Pages: 342-347
Peer Reviewed
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[Journal Article] Glycerol kinase activity and glycerol kinase-3 gene are up-regulated by acclimation to 5℃in diapause eggs of the silkworm2009
Author(s)
Kihara, F., Itoh, K., Iwasaka, M., Niimi, T., Yamashita, O. and Yaginuma, T
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Journal Title
Bombyx mori. Insect Biochem. Mol. Biol
Volume: 39
Pages: 763-769
Peer Reviewed
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[Presentation] Bombyx mori embryonic diapause : transducers of diapause hormone signal for diapause initiation and 5℃signal for diapause termination2011
Author(s)
Yaginuma, T., Suzuki, A., Kihara, F., Kamei, Y., Niimi, T., Yamashita, O
Organizer
The Sixth International Symposium on Molecular Insect Science
Place of Presentation
アムステルダム
Year and Date
20111002-05
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