Molecular studies on the self-incompatibility systems in the Brassicaceae and the Solanaceae

2011 Fiscal Year Final Research Report

• Project/Area Number: 21248014
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: TAKAYAMA Seiji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70273836)
• Co-Investigator(Renkei-kenkyūsha): HIRANO Yoshinori 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50452529) ; MAESHIMA Masayoshi 名古屋大学, 生命農学研究科, 教授 (80181577) ; NAGAI Takeharu 大阪大学, 産業科学研究所, 教授 (20311350) ; NAKAZONO Mikio 名古屋大学, 生命農学研究科, 教授 (70282697) ; KURATA Nori 国立遺伝学研究所, 系統生物研究センター, 教授 (90178088)
• Project Period (FY): 2009 – 2011
• Keywords: 情報伝達 / 植物生殖 / 自家不和合性 / アブラナ科 / ナス科

Research Abstract

This study aimed to reveal the molecular mechanisms of self-incompatibility in the Brassicaceae and the Solanaceae. In the Brassicaceae, we have analyzed the downstream signaling cascades after the perception of the pollen determinant(SP11 ligand) by the pistil determinant(SRK receptor kinase), and identified some candidate molecules and some mutants related to the cascades. In the Solanaceae, we have revealed that the pollen determinant comprises multiple types of F-box proteins(SLFs), and proposed the "collaborative non-self recognition model" suggesting that SLFs collectively recognize and detoxify entire suite of non-self S-RNases.

Research Products

• [Journal Article] Self/non-self discrimination in angiosperm self-incompatibility (2012)
  • Author(s): Iwano, M., and Takayama, S.
  • Journal Title: Curr. Opin. Plant Biol Volume: 15 Pages: 78-83
  • Peer Reviewed
• [Journal Article] Epigenetic regulation of monoallelic gene expression (2012)
  • Author(s): Shiba, H., and Takayama, S.
  • Journal Title: Develop. Growth Differ Volume: 54 Pages: 120-128
  • Peer Reviewed
• [Journal Article] Monoallelic gene expression and its mechanisms (2011)
  • Author(s): Tarutani, Y., and Takayama, S.
  • Journal Title: Curr. Opin. Plant Biol Volume: 14 Pages: 608-613
  • Peer Reviewed
• [Journal Article] Collaborative non-self recognition system in S-RNase-based self-incompatibility (2010)
  • Author(s): Kubo, K., Entani, T., Takara, A., Wang, N., Fields, A. M., Hua, Z., Toyoda, M., Kawashima, S., Ando, T., Isogai, A., Kao, T.-h., and Takayama, S.
  • Journal Title: Science Volume: 330 Pages: 796-799
  • Peer Reviewed
• [Journal Article] Trans-acting small RNA determines dominance relationships in Brassica self-incompatibility (2010)
  • Author(s): Tarutani, Y., Shiba, H., Iwano, M., Kakizaki, T., Suzuki, G., Watanabe, M., Isogai, A., and Takayama, S.
  • Journal Title: Nature Volume: 466 Pages: 983-986
  • Peer Reviewed
• [Journal Article] Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene (2010)
  • Author(s): Tsuchimatsu, T., Suwabe, K., Shimizu-Inatsugi, R., Isokawa, S., Pavlidis, P., Stadler, T., Suzuki, G., Takayama, S., Watanabe, M., and Shimizu, K. K.
  • Journal Title: Nature Volume: 464 Pages: 1342-1346
  • Peer Reviewed
• [Journal Article] Novel self-compatible lines of Brassica rapa L. isolated from the Japanese bulk-populations (2010)
  • Author(s): Isokawa, S., Osaka, M., Shirasawa, A., Kikuta, R., Komatsu, S., Horisaki, A., Niikura, S., Takada, Y., Shiba, H., Isogai, A., Takayama, S., Suzuki, G., Suwabe, K., and Watanabe, M.
  • Journal Title: Genes Genet. Syst Volume: 85 Pages: 87-96
  • Peer Reviewed
• [Journal Article] Fine-tuning of the cytoplasmic Ca^<2+> concentration is essential for pollen tube growth (2009)
  • Author(s): Iwano, M., Entani, T., Shiba, H., Kakita, M., Nagai, T., Miyawaki, A., Shoji, T., Kubo, K., Isogai, A., and Takayama, S.
  • Journal Title: Plant Physiol Volume: 150 Pages: 1-13
  • Peer Reviewed
• [Presentation] Noncoding RNA and epigenetics in self, non-self recognition in fertilization (2012)
  • Author(s): Tarutani, Y., Miura, E., Shiba, H., and Takayama, S.
  • Organizer: Keystone Symposia on Molecular and Cellular Biology "Nuclear Events in Plant Gene Expression and Signaling"
  • Place of Presentation: Taos, USA
  • Year and Date: 2012-03-10
• [Presentation] Molecular mechanisms of self-incompatibility in Brassicaceae (2012)
  • Author(s): Iwano, M., Ito, K., and Takayama, S.
  • Organizer: 22nd International Congress of Sexual Plant Reproduction
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2012-02-14
• [Presentation] Epigenetic regulation of dominance relationships in Brassica self-incompatibility alleles (2011)
  • Author(s): Shiba, H., Tarutani, Y., Miura, E., Watanabe, M., Isogai, A., and Takayama, S.
  • Organizer: Workshop in The 34th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-13
• [Presentation] Collaborative non-self recognition system in S-RNase-based self-incompatibility (2011)
  • Author(s): Kubo, K., Entani, T., and Takayama, S.
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Joint Conference(SOL & ICuGI 2011)
  • Place of Presentation: Kobe
  • Year and Date: 2011-11-30
• [Presentation] Self/non-self discrimination in plant self-incompatibility (2011)
  • Author(s): Takayama, S.
  • Organizer: NAIST Global COE International Symposium "Achievements and Future"
  • Place of Presentation: Nara
  • Year and Date: 2011-11-08
• [Presentation] Regulation of dominance relationships in Brassica self-incompatibility alleles (2010)
  • Author(s): Tarutani, Y., Shiba, H., Iwano, M., Kakizaki, T., Suzuki, G., Watanabe, M., Isogai, A., and Takayama, S.
  • Organizer: Cold Spring Harbor Asia Conference
  • Place of Presentation: Suzhou, China
  • Year and Date: 2010-10-27
• [Presentation] Molecular mechanisms of self-incompatibility in the Brassicaceae (2010)
  • Author(s): Takayama, S.
  • Organizer: XXI International Congress on Sexual Plant Reproduction(21st ICSPR)
  • Place of Presentation: Bristol, UK
  • Year and Date: 2010-08-05
• [Presentation] Analysis of S-locus F-box genes in Petunia (2010)
  • Author(s): Kubo, K., Entani, T., Takara, A., Isogai, A., Kao, T.-h., and Takayama, S.
  • Organizer: XXI International Congress on Sexual Plant Reproduction(21st ICSPR)
  • Place of Presentation: Bristol, UK
  • Year and Date: 2010-08-05
• [Presentation] Genome-wide analyses of allele-specific expression and DNA methylation in Arabidopsis thaliana (2010)
  • Author(s): Shiba, H., Kayabe, T., Yano, K., Yazaki, J., Chen, H., Suzuki, Y., Fujita, M., Kurata, N., Isogai, A., Ecker, J. R., and Takayama, S.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama
  • Year and Date: 2010-06-08
• [Presentation] Self-incompatibility signaling in the Brassicaceae (2010)
  • Author(s): Takayama, S.
  • Organizer: International Symposium of Cell-Cell Communication in Plant Reproduction-from pollination to fertilization-
  • Place of Presentation: Nara
  • Year and Date: 2010-03-11
• [Presentation] Regulation of dominance relationships between self-incompatibility alleles in Brassica via de novo DNA methylation (2010)
  • Author(s): Tarutani, Y., Shiba, H., Iwano, M., Kakizaki, T., Suzuki, G., Watanabe, M., Isogai, A., and Takayama, S.
  • Organizer: International Symposium of Cell-Cell Communication in Plant Reproduction-from pollination to fertilization-
  • Place of Presentation: Nara
  • Year and Date: 2010-03-11
• [Presentation] Analysis of the self-incompatibility mechanisms in Petunia (2010)
  • Author(s): Kubo, K., Entani, T., Kawashima, S., Watanabe, H., Ando, T., Kao, T.-h., Isogai, A., and Takayama, S.
  • Organizer: International Symposium of Cell-Cell Communication in Plant Reproduction-from pollination to fertilization-
  • Place of Presentation: Nara
  • Year and Date: 2010-03-11
• [Presentation] Evolution of breeding systems in Arabidopsis relatives (2010)
  • Author(s): Tsuchimatsu, T., Suwabe, K., Shimizu-Inatsugi, R., Isokawa, S., Pavlidis, P., Stadler, T., Suzuki, G., Takayama, S., Watanabe, M., and Shimizu, K. K.
  • Organizer: International Symposium of Cell-Cell Communication in Plant Reproduction-from pollination to fertilization-
  • Place of Presentation: Nara
  • Year and Date: 2010-03-11
• [Presentation] Unilateral incompatibility in Brassica rapa leading to a novel pollen-stigma recognition mechanism (2010)
  • Author(s): Takada, Y., Suzuki, G., Shiba, H., Takayama, S., Isogai, A., and Watanabe, M.
  • Organizer: International Symposium of Cell-Cell Communication in Plant Reproduction-from pollination to fertilization-
  • Place of Presentation: Nara
  • Year and Date: 2010-03-11
• [Presentation] Novel self-compatible lines of Brassica rapa L. isolated from the Japanese bulk-populations (2010)
  • Author(s): Osaka, M., Isokawa, S., Shirasawa, A., Kikuta, R., Komatsu, S., Niikura, S., Takada, Y., Shiba, H., Isogai, A., Takayama, S., Suzuki, G., Suwabe, K., and Watanabe, M.
  • Organizer: International Symposium of Cell-Cell Communication in Plant Reproduction-from pollination to fertilization-
  • Place of Presentation: Nara
  • Year and Date: 2010-03-11
• [Presentation] Self-incompatibility signaling in the Brassicaceae (2009)
  • Author(s): Takayama, S.
  • Organizer: 9th International Plant Molecular Biology Congress(IPMB)
  • Place of Presentation: St. Louis, USA
  • Year and Date: 2009-10-28
• [Presentation] Fine tuning of the cytoplasmic calcium concentration is essential for pollen tube growth (2009)
  • Author(s): Iwano, M., Isogai, A., and Takayama, S.
  • Organizer: 9th International Plant Molecular Biology Congress(IPMB)
  • Place of Presentation: St. Louis, USA
  • Year and Date: 2009-10-28
• [Presentation] Regulation of dominance relationships between self-incompatibility alleles via de novo DNA methylation (2009)
  • Author(s): Tarutani, Y., Shiba, H., Iwano, M., Kakizaki, T., Watanabe, M., Isogai, A., and Takayama, S.
  • Organizer: 9th International Plant Molecular Biology Congress(IPMB)
  • Place of Presentation: St. Louis, USA
  • Year and Date: 2009-10-28
• [Presentation] Three dimensional analysis of the vacuolar structure in papilla cells during selfand cross-pollination in Brassicaceae (2009)
  • Author(s): Iwano, M., Matoba, K., Hasegawa, T., Takaoka, A., Isogai, A., and Takayama, S.
  • Organizer: 6th International Symposium on Electron Microscopy in Medicine and Biology 2009
  • Place of Presentation: Kobe
  • Year and Date: 2009-07-17
• [Presentation] Three-dimensional analysis of intracellular organelles in a papilla cell of Brassica rapa (2009)
  • Author(s): Nagai, R., Iwano, M., Kajimura, N., Hasegawa, T., Takaoka, A., Isogai, A., and Takayama, S.
  • Organizer: 6th International Symposium on Electron Microscopy in Medicine and Biology 2009
  • Place of Presentation: Kobe
  • Year and Date: 2009-07-17
• [Presentation] Genetic characterization of self-compatible mutants in Brassica rapa (2009)
  • Author(s): Suwabe, K., Isokawa, S., Shirasawa, A., Takada, Y., Suzuki, G., Isogai, A., Takayama S., and Watanabe, M.
  • Organizer: Plant Biology 2009
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2009-07-17
• [Presentation] Unilateral incompatibility in Brassica rapa lead to a novel pollen-stigma recognition mechanism (2009)
  • Author(s): Takada, Y., Suzuki, G., Shiba, H., Takayama, S., Isogai, A., and Watanabe, M.
  • Organizer: Plant Biology 2009
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2009-07-17
• [Book] Self-incompatibility systems in flowering plants In'' Plant Developmental Biology-Biotechnological Perspectives : Volume 1"(Edited by Pua, E. C., and Davey, M. R.) (2009)
  • Author(s): Nakayama, P., Isogai, A., and Takayama, S.
  • Total Pages: 459-485
  • Publisher: Springer, Heidelberg, Germany
• [Remarks] ホームページアドレス
  • URL: http://bsw3.naist.jp/takayama/index.html