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2012 Fiscal Year Final Research Report

Structural elucidation of nucleus and chromosome using non-linear optical microscopy

Research Project

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Project/Area Number 21248040
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionOsaka University

Principal Investigator

FUKUI Kiichi  大阪大学, 工学研究科, 教授 (00311770)

Co-Investigator(Kenkyū-buntansha) OZEKI Yasuyuki  大阪大学, 大学院・工学研究科, 助教 (60437374)
ITO Kazuyoshi  大阪大学, 大学院・工学研究科, 教授 (80113520)
UCHIYAMA Susumu  大阪大学, 大学院・工学研究科, 准教授 (90335381)
Project Period (FY) 2009 – 2012
Keywords非線形光学顕微鏡 / 染色体観察 / 低侵襲無染色 3 次元観察
Research Abstract

Non-labeling and 3D visualization of nuclear and chromosomal structures were achieved by using newly developed non-linear optical microscopic techniques, such as Stimulated Raman Scattering(SRS) microscopy and Stimulated Parametric Emission(SPE) microscopy.

  • Research Products

    (20 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (7 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Visualization of Oil Body Distribution in Jatropha curcas L. by Four-Wave Mixing Microscopy2013

    • Author(s)
      Ishii M., Uchiyama S., Ozeki Y., Kajiyama S., Itoh, K. and Fukui K.
    • Journal Title

      Jpn. J. Appl. Phys.

      Volume: Vol 52 Pages: 062403-062407

    • Peer Reviewed
  • [Journal Article] Sensitivity enhancement of fiber-laser-based stimulated Raman scattering microscopy by collinear balanced detection technique2012

    • Author(s)
      Nose K., Ozeki Y., Kishi T., Sumimura K., Nishizawa N., Fukui K., Kanematsu Y., and Itoh K.
    • Journal Title

      Optics Express

      Volume: Vol. 20 Pages: 13958-13965

    • Peer Reviewed
  • [Journal Article] High-speed molecular spectral imaging of tissue with stimulated Raman scattering2012

    • Author(s)
      Ozeki Y., Umemura W., Otsuka Y., Satoh S., Hashimoto H., Sumimura K., Nishizawa N., Fukui K. and Itoh K.
    • Journal Title

      Nature Photonics

      Volume: Vol.6 Pages: 845-851

    • Peer Reviewed
  • [Journal Article] Stimulated Raman hyperspectral imaging based on spectral filtering of broadband laser pulses2012

    • Author(s)
      Ozeki Y., Umemura W., Sumimura K., Nishizawa N., Fukui K., and Itoh K.
    • Journal Title

      Optics Letters

      Volume: Vol. 37 Pages: 431-433

    • Peer Reviewed
  • [Journal Article] Subharmonic Synchronization of Picosecond Yb Fiber Laser to Picosecond Ti:Sapphire Laser for Stimulated Raman Scattering Microscopy2012

    • Author(s)
      Umemura W., Fujita K., Ozeki Y., Goto K., Sumimura K., Nishizawa N., Fukui K., Itoh K.
    • Journal Title

      Jpn. J. Appl. Phys.

      Volume: Vol. 51 Pages: 022702-022705

    • Peer Reviewed
  • [Journal Article] The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA2010

    • Author(s)
      Hayashihara K., Uchiyama S., Shimamoto S., Kobayashi S., Tomschik M., Wakamatsu H., No D., Sugahara H., Hori N., Noda M., Ohkubo T., Zlatanova J., Matsunaga S. and Fukui K.
    • Journal Title

      J. Biol. Chem.

      Volume: Vol. 285 Pages: 6498-6507

    • Peer Reviewed
  • [Journal Article] Stimulated Raman scattering microscopy for live cell imaging with high contrast and high sensitivity2010

    • Author(s)
      Ozeki Y. and Itoh K.
    • Journal Title

      Laser Phys.

      Volume: Vol. 20 Pages: 1114-1118

    • Peer Reviewed
  • [Journal Article] Dual-Band Stimulated Parametric Emission Microscopy2010

    • Author(s)
      Yamagiwa M., Omura G., Ozeki Y., Ishii M., H. M. Dang, Kajiyama S., Suzuki T., Fukui K., and Itoh K.
    • Journal Title

      Jpn. J. Appl. Phys.

      Volume: Vol. 49 Pages: 16603-16606

    • Peer Reviewed
  • [Journal Article] Three-Dimensional Unstained Live-Cell Imaging Using Stimulated Parametric Emission Microscopy2010

    • Author(s)
      H. M. Dang, Kawasumi T., Omura G., Umano T., Kajiyama S., Ozeki Y., Itoh K., and Fukui K.
    • Journal Title

      Jpn. J. Appl. Phys.

      Volume: Vol.48 Pages: 97003-97007

    • Peer Reviewed
  • [Journal Article] Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes2009

    • Author(s)
      H. M. Dang, Omura G., Umano T., Yamagiwa M., Kajiyama S., Ozeki Y., Itoh K., Fukui K.
    • Journal Title

      J. Biomed. Opt.

      Volume: Vol.14 Pages: 040506-040508

    • Peer Reviewed
  • [Presentation] Label-free observation of tissues by high-speed stimulated Raman spectral microscopy and independent component analysis2013

    • Author(s)
      Ozeki Y., Otsuka Y., Sato S., Hashimoto H., Umemura W., Sumimura K., Nishizawa N., Fukui K., and Itoh K.
    • Organizer
      Photonics West 2013
    • Place of Presentation
      San Francisco
    • Year and Date
      2013-02-03
  • [Presentation] 誘導ラマン散乱を用いた無染色生体顕微鏡2012

    • Author(s)
      小関泰之
    • Organizer
      第50回 光波センシング技術研究会
    • Place of Presentation
      東京理科大学
    • Year and Date
      2012-12-05
  • [Presentation] High-Speed Molecular Spectral Imaging by Stimulated Raman Scattering Microscopy Using Wavelength-Tunable Pulses2012

    • Author(s)
      Wataru Umemura, Yasuyuki Ozeki, Kenta Fujita, Kazuhiko Sumimura, Norihiko Nishizawa, Kiichi Fukui, Kazuyoshi Itoh
    • Organizer
      CLEO 2012
    • Place of Presentation
      San Jose
    • Year and Date
      2012-05-09
  • [Presentation] 誘導ラマン散乱による生体イメージングの実証とその高感度化2011

    • Author(s)
      小関泰之
    • Organizer
      春季 第58回 応用物理学関係連合講演会
    • Place of Presentation
      神奈川工科大学
    • Year and Date
      2011-03-24
  • [Presentation] 誘導ラマン散乱顕微鏡による高速分光イメージング2011

    • Author(s)
      梅村航,小関泰之,藤田健太,住村和彦,西澤典彦,福井希一,伊東一良
    • Organizer
      第59回応用物理学関係連合講演会
    • Place of Presentation
      早稲田大学
    • Year and Date
      2011-03-15
  • [Presentation] 高調波同期した 2 波長光源による誘導ラマン散乱顕微鏡の高感度化2010

    • Author(s)
      小関泰之,北川雄真,梅村航,住村和彦,西澤典彦,梶山慎一郎,福井希一,伊東一良
    • Organizer
      第5回超高速光エレクトロニクス研究会
    • Place of Presentation
      慶応大学日吉キャンパス
    • Year and Date
      2010-08-20
  • [Presentation] Stimulated Raman scattering microscopy for live-cell imaging with high contrast and high sensitivity2009

    • Author(s)
      Ozeki Y. and Itoh K.
    • Organizer
      18th International Laser Physics Workshop (LPHYS'09)
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2009-07-16
  • [Remarks]

    • URL

      http://www.bio.eng.osaka-u.ac.jp/cl/top.php

  • [Remarks] 日刊工業新聞、2009年6月8日「SRS 顕微鏡を開発 生きた細胞 3次元観察」

  • [Patent(Industrial Property Rights)] 光学顕微鏡、および光学計測2010

    • Inventor(s)
      小関 泰之,伊東 一良,嶽 文宏,福井 希一,梶山 慎一郎,北川 雄真,西澤 典彦,住村 和彦
    • Industrial Property Rights Holder
      キヤノン株式会社
    • Industrial Property Number
      PCT/JP2010/059327
    • Filing Date
      2010-06-02
    • Overseas

URL: 

Published: 2014-08-29  

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