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2011 Fiscal Year Final Research Report

Mechanism of adrenocortical differentiation by exchange and conversion of enhancers

Research Project

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Project/Area Number 21249018
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKyushu University

Principal Investigator

MOROHASHI Ken-ichirou  九州大学, 大学院・医学研究院, 主幹教授 (30183114)

Project Period (FY) 2009 – 2011
Keywords副腎皮質 / エンハンサー / クロマチン構造
Research Abstract

Ad4BP/SF-1, a member of nuclear receptor superfamily, plays pivotal role in differentiation and function of the adrenal cortex through regulating various target genes. It has been hypothesized that active enhancers should be exchanged or selected through conversion of chromatin structure when Ad4BP/SF-1 functions during the tissue development. In the present study, we performed ChIP-sequence analyses of theDNA recovered with antibodies for Ad4BP/SF-1 and modified histones. As the consequence, it was found that Ad4BP/SF-1 regulates glycolytic gene expression through binding to enhancers localized in and around the genes.

  • Research Products

    (27 results)

All 2012 2011 2010 2009 Other

All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (13 results) Remarks (1 results)

  • [Journal Article] WNT family genes and their modulation in the ovary-independent and persistent vaginal epithelial cell proliferation and keratinization induced by neonatal diethylstilbesterol exposure in mice2012

    • Author(s)
      T. Nakamura, S. Miyagawa, Y. Katsu, H. Watanabe, T. Mizutani, T. Sato, K. Morohashi, T. Takeuchi, T. Iguchi.
    • Journal Title

      Toxicology

      Volume: 296 Pages: 13-19

    • Peer Reviewed
  • [Journal Article] Cbx2, a polycomb group gene, is required for Sry gene expression in mice2012

    • Author(s)
      Y. Katoh-Fukui, K. Miyabayashi, T. Komatsu, A. Owaki, T. Baba, Y. Shima, T. Kidokoro, Y. Kanai, A. Schedl, D. Wilhelm, P. Koopman, Y. Okuno, K. Morohashi.
    • Journal Title

      Endocrinology

      Volume: 153 Pages: 913-924

    • Peer Reviewed
  • [Journal Article] Identification of enhancer specific for fetal Leydig cells in Ad4BP/SF-1 gene2012

    • Author(s)
      Y. Shima, K. Miyabayashi, T. Baba, H. Otake, S. Oka, M. Zubair, K. Morohashi.
    • Journal Title

      Endocrinology

      Volume: 153 Pages: 417-425

    • Peer Reviewed
  • [Journal Article] SF-1 expression during adrenal development and tumorigenesis. Gardiner JR2012

    • Author(s)
      Y Shima, K. Morohashi, A. Swain
    • Journal Title

      Mol. Cell. Endocrinol

      Volume: 351 Pages: 12-18

    • Peer Reviewed
  • [Journal Article] DNA methylation of intronic enhancers directs tissue-specific expression of Steroidogenic Factor 1/Adrenal 4 Binding Protein2011

    • Author(s)
      E. A. Hoivik, T. E. Bjanesoy, O. Mai, S. Okamoto, Y. Minokoshi, Y. Shima, K. Morohashi, U. Boehm, and M. Bakke
    • Journal Title

      Endocrinology

      Volume: 152 Pages: 2100-2112

    • Peer Reviewed
  • [Journal Article] The fetal and adult adrenal cortex2011

    • Author(s)
      K. Morohashi and M. Zubair.
    • Journal Title

      Mol. Cell. Endocrinol.

      Volume: 336 1 Pages: 193-197

    • Peer Reviewed
  • [Journal Article] Abnormal epithelial cell polarity and migration of Emx2 KO embryonic gonads induced by ectopic EGFR expression2010

    • Author(s)
      M. Kusaka, Y. Katoh-Fukui, H. Ogawa, K. Miyabayashi, T. Baba, N. Sugiyama, Y. Sugimoto, Y. Okuno, R. Kodama, A. Iizuka-Kogo, T. Senda, S. Aizawa, K. Morohashi.
    • Journal Title

      Endocrinol

      Volume: 151 Pages: 5893-5904

    • Peer Reviewed
  • [Journal Article] Production and characterization of monoclonal antibodies to germ cells2010

    • Author(s)
      C. Yokoyama, Y. Katoh-Fukui, K. Morohashi, D. Konno, M. Azauma, and T. Tachibana.
    • Journal Title

      Hybridoma

      Volume: 29 Pages: 53-57

    • Peer Reviewed
  • [Journal Article] Transcriptional suppression by transient recruitment of ARIP4 to sumoylated nuclear receptor Ad4BP/SF-12009

    • Author(s)
      H. Ogawa, T. Komatsu, Y. Hiraoka, and K. Morohashi.
    • Journal Title

      Mol. Biol. Cell

      Volume: 20 Pages: 4235-4245

    • Peer Reviewed
  • [Journal Article] Transgenic expression of Ad4BP/SF-1 in fetal adrenal progenitor cells leads to ectopic adrenal formation2009

    • Author(s)
      M. Zubair, S. Oka, K. L. Parker, and K. Morohashi
    • Journal Title

      Mol Endocrinol

      Volume: 23 Pages: 1657-1667

    • Peer Reviewed
  • [Journal Article] Generation of rat monoclonal antibodies specific for Ad4BP/SF-12009

    • Author(s)
      C. Yokoyama, T. Komatsu, H. Ogawa, K. Morohashi, M. Azuma, and T. Tachibana
    • Journal Title

      Hybridoma

      Volume: 28 Pages: 113-119

  • [Journal Article] A critical time window of Sry action in gonadal sex determination2009

    • Author(s)
      R. Hiramatsu, S. Matoba, M. Fujisawa, M. Kanai-Azuma, N. Tsunekawa, M. Kurohmaru, K. Morohashi, D. Wilhelm, P. Koopman, Y. Kanai.
    • Journal Title

      Development

      Volume: 136 Pages: 129-138

    • Peer Reviewed
  • [Journal Article] Steroid hormones and the development of reproductive organs

    • Author(s)
      K. Morohashi, M. Tanaka
    • Journal Title

      Sexual Development. in press. Review

    • Peer Reviewed
  • [Presentation] 胎仔型ライディッヒ細胞の生理学的機能の解明2011

    • Author(s)
      嶋雄一、諸橋憲一郎
    • Organizer
      第84回日本内分泌学会
    • Place of Presentation
      神戸
    • Year and Date
      20110421-23
  • [Presentation] 生殖腺におけるステロイドホルモン産生2011

    • Author(s)
      諸橋憲一郎
    • Organizer
      第6回日本生殖内分泌学会
    • Place of Presentation
      東京
    • Year and Date
      2011-11-19
  • [Presentation] Transcriptomic analysis of fetal Leydig cells2010

    • Author(s)
      Y. Shima, T. Baba, K. Miyabayashi, H. Otake, Y. Kurihara, K. Morohashi
    • Organizer
      第33回日本分子生物学会、第83回日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-10
  • [Presentation] The Keith Parker Memorial Lecture. Ad4BP/SF-1 as the key factor for steroidogenic cell development2010

    • Author(s)
      K. Morohashi
    • Organizer
      14th Adrenal Cortex Conference
    • Place of Presentation
      San Diego, USA
    • Year and Date
      20100916-18
  • [Presentation] ステロイド産生細胞の発生2010

    • Author(s)
      諸橋憲一郎
    • Organizer
      第28回内分泌代謝サマーセミナー
    • Place of Presentation
      長崎
    • Year and Date
      20100708-10
  • [Presentation] Implication of Ad4BP/SF-1 into Adrenal Development2010

    • Author(s)
      K. Morohashi, Y. Shima, K. Miyabayashi, H. Otake, T. Baba, M. Zubair
    • Organizer
      14th International Congress of Endocrinology, ICE2010
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20100326-30
  • [Presentation] 生殖腺の性差構築2010

    • Author(s)
      諸橋憲一郎
    • Organizer
      第4回日本性差医学・医療学会
    • Place of Presentation
      下関
    • Year and Date
      20100205-06
  • [Presentation] Tissue-specific Enhancers of Ad4BP/SF-12010

    • Author(s)
      K. Morohashi
    • Organizer
      Gene Official Satellite Symposia of ICE2010
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2010-03-30
  • [Presentation] 生殖腺の発生と性分化のメカニズム2009

    • Author(s)
      諸橋憲一郎、馬場崇、宮林香奈子、大竹博之、嶋雄一
    • Organizer
      第54回日本生殖医学会
    • Place of Presentation
      金沢
    • Year and Date
      20091122-23
  • [Presentation] Molecular mechanisms for the functions and structures of tissue specific enhancers2009

    • Author(s)
      Y. Shima, T. Baba, K. Miyabayashi, M. Zubair, K. Morohashi
    • Organizer
      Fifth international Symposium on the biology of vertebrate sex determination
    • Place of Presentation
      Kona, Hawaii, USA
    • Year and Date
      20090420-24
  • [Presentation] Genetic Program of Gonad Differenti2009

    • Author(s)
      諸橋憲一郎
    • Organizer
      第73回日本循環器学会
    • Place of Presentation
      大阪
    • Year and Date
      20090320-22
  • [Presentation] Aug 15-19, Andover, Andover, New Hampshire

    • Author(s)
      Keynote Lecture, Molecular and Cellular Mechanisms of Testis Differentiation. K. Morohashi
    • Organizer
      Gordon Research Conference, 2010 Reproductive Tract Biology
    • Place of Presentation
      USA
  • [Presentation] Milwaukee

    • Author(s)
      Lineage of Testicular Leydig cells and Adrenal Cortex. K. Morohashi, Z. Mohamad, T. Baba, K. Miyabayashi, H. Otake, Y. Shima
    • Organizer
      43 rd Annual Meeting of the Society for the Study of Reproduction July 28-Aug
    • Place of Presentation
      USA, Invited Speaker
  • [Remarks]

    • URL

      http://www.med.kyushu-u.ac.jp/seisaseibutu/

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Published: 2013-07-31  

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