2011 Fiscal Year Final Research Report
Identification of RBC receptors against malaria parasite molecules in the merozoite apical organelle
| Project/Area Number |
21249028
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Ehime University |
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Principal Investigator |
TSUBOI Takafumi 愛媛大学, 無細胞生命科学工学研究センター, 教授 (00188616)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEO Satoru 杏林大学, 医学部, 准教授 (40302666)
SAWASAKI Tatsuya 愛媛大学, 無細胞生命科学工学研究センター, 准教授 (50314969)
OTSUKI Hitoshi 鳥取大学, 医学部, 講師 (80403806)
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| Project Period (FY) |
2009 – 2011
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| Keywords | マラリア / レセプター / コムギ胚芽無細胞タンパク質合成系 / ゲノム |
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| Research Abstract |
In order to identify novel molecules localized in the apical organelles of Plasmodium merozoite, we have expressed 193 putative merozoite specific molecules and produced antibodies against each recombinant protein. Among them, 29 were localized at the apical end of the merozoite suggesting novel apical organellar proteins. We then characterized the RBC binding activity and the RBC receptor phenotype against one of the novel micronemal molecules, GAMA. Furthermore, we have constructed erythrocyte protein array including 500 of molecules, and identified an erythrocyte membrane protein which serves as a receptor against erythrocyte-binding rhoptry protein.
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